BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0006 (609 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 83 2e-16 At5g63960.1 68418.m08031 DNA-directed DNA polymerase delta catal... 36 0.028 At4g28130.1 68417.m04033 diacylglycerol kinase accessory domain-... 27 7.4 At1g77470.1 68414.m09021 replication factor C 36 kDA, putative s... 27 7.4 At2g33860.1 68415.m04157 auxin-responsive factor (ARF3) / ETTIN ... 27 9.7 >At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catalytic subunit, putative similar to SP|O48653 DNA polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza sativa}; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain Length = 1492 Score = 82.6 bits (195), Expect = 2e-16 Identities = 45/95 (47%), Positives = 60/95 (63%), Gaps = 8/95 (8%) Frame = +3 Query: 255 VALRLNSKNSRR-FKKGDIVPYIIC-EDGTANSAT-----QRAYHIEELKNS-EHLKVDF 410 VALR+ + + F D VPYIIC E G A+SA+ +RA H +E+K+ VD Sbjct: 1200 VALRMRQRGYKEGFNAKDTVPYIICYEQGNASSASSAGIAERARHPDEVKSEGSRWLVDI 1259 Query: 411 KYYLAHQLHPVISRICEPIEGMDPARVADCLGLIP 515 YYLA Q+HPV+SR+C I+G P R+A+CLGL P Sbjct: 1260 DYYLAQQIHPVVSRLCAEIQGTSPERLAECLGLDP 1294 Score = 71.3 bits (167), Expect = 5e-13 Identities = 35/82 (42%), Positives = 51/82 (62%) Frame = +1 Query: 10 RGLDIVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKDDLIGNKMPLSVLA 189 +G+D+VR DWS L+ E G LS+IL + +D +E+I N L K+K+++ ++ L Sbjct: 1118 KGVDMVRRDWSLLSKEIGDLCLSKILYGGSCEDVVEAIHNELMKIKEEMRNGQVALEKYV 1177 Query: 190 ITKQLTKNPNEYADKHTQSHVQ 255 ITK LTK P Y D +Q HVQ Sbjct: 1178 ITKTLTKPPAAYPDSKSQPHVQ 1199 >At5g63960.1 68418.m08031 DNA-directed DNA polymerase delta catalytic subunit, putative (POLD1) similar to DNA polymerase delta [Glycine max] GI:2895198, OsPol delta large subunit [Oryza sativa (japonica cultivar-group) GI:9188570; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain Length = 1081 Score = 35.5 bits (78), Expect = 0.028 Identities = 23/75 (30%), Positives = 41/75 (54%) Frame = +1 Query: 10 RGLDIVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKDDLIGNKMPLSVLA 189 +G++ VR D L L++IL ++ E+++ ++ DL+ N++ LS+L Sbjct: 822 KGIETVRRDNCLLVKNLVTESLNKILIDRDVPGAAENVKKTIS----DLLMNRIDLSLLV 877 Query: 190 ITKQLTKNPNEYADK 234 ITK LTK ++Y K Sbjct: 878 ITKGLTKTGDDYEVK 892 Score = 33.5 bits (73), Expect = 0.11 Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 1/57 (1%) Frame = +3 Query: 300 GDIVPYIICEDGTANSATQRAYH-IEELKNSEHLKVDFKYYLAHQLHPVISRICEPI 467 GD VPY+I + A +R+ I L+N+ + +D YYL +Q+ + RI EP+ Sbjct: 914 GDRVPYVIIKAAKGAKAYERSEDPIYVLQNN--IPIDPNYYLENQISKPLLRIFEPV 968 >At4g28130.1 68417.m04033 diacylglycerol kinase accessory domain-containing protein similar to diacylglycerol kinase [Lycopersicon esculentum] GI:10798892; contains Pfam profile PF00609: Diacylglycerol kinase accessory domain (presumed) Length = 466 Score = 27.5 bits (58), Expect = 7.4 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +1 Query: 85 LSEQTPDDRLESIQNHLNKLKDDLIGNKM 171 L +TPD L+ I +L +LKDD + +K+ Sbjct: 80 LEVETPDKVLQRIYLNLERLKDDSLASKI 108 >At1g77470.1 68414.m09021 replication factor C 36 kDA, putative similar to SWISS-PROT:P40937 activator 1 36 kDa subunit (Replication factor C 36 kDa subunit, A1 36 kDa subunit, RF-C 36 kDa subunit, RFC36) [Homo sapiens] Length = 369 Score = 27.5 bits (58), Expect = 7.4 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +1 Query: 22 IVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKL 144 +V LD + + +F L +++ + T R I NH+NK+ Sbjct: 141 LVLLDEADAMTKDAQFALRRVIEKYTKSTRFALIGNHVNKI 181 >At2g33860.1 68415.m04157 auxin-responsive factor (ARF3) / ETTIN protein (ETT) identical to ETTIN GB:AF007788 from [Arabidopsis thaliana] Length = 608 Score = 27.1 bits (57), Expect = 9.7 Identities = 13/27 (48%), Positives = 14/27 (51%) Frame = +2 Query: 419 SSTSATSCNIKNMRTNRRHGSSPRCRL 499 SS T+ N N R HG S RCRL Sbjct: 526 SSVLLTNSNSPNGRLEDHHGGSGRCRL 552 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,739,988 Number of Sequences: 28952 Number of extensions: 255700 Number of successful extensions: 698 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 680 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 697 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1216725696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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