BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmov11p12 (706 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 96 2e-20 At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 92 3e-19 At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 92 4e-19 At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 91 9e-19 At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 88 5e-18 At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 88 6e-18 At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 86 2e-17 At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 63 2e-10 At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 61 6e-10 At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 54 7e-08 At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 52 5e-07 At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 33 0.18 At2g32350.1 68415.m03954 ubiquitin family protein contains INTER... 29 2.3 At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04... 28 5.2 At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 27 9.2 At3g44820.1 68416.m04829 phototropic-responsive NPH3 family prot... 27 9.2 At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 27 9.2 >At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cytochrome B5 SP:P49098 from [Nicotiana tabacum] Length = 134 Score = 96.3 bits (229), Expect = 2e-20 Identities = 41/78 (52%), Positives = 54/78 (69%) Frame = +1 Query: 208 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 387 +K TL EV +H WI+I+ VY+VTKFLE+HPGG D LL GKD T FEDVGH Sbjct: 5 AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64 Query: 388 SDDARELLKKYKIGTLPP 441 S+ ARE++++Y +G + P Sbjct: 65 SESAREMMEQYYVGEIDP 82 >At1g26340.1 68414.m03212 cytochrome b5, putative similar to cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis thaliana] Length = 135 Score = 92.3 bits (219), Expect = 3e-19 Identities = 39/77 (50%), Positives = 53/77 (68%) Frame = +1 Query: 205 MSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVG 384 ++K +++E H + W++I VYDV+ +++EHPGG D LL VAGKD T FED G Sbjct: 4 LTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAG 63 Query: 385 HSDDARELLKKYKIGTL 435 HS DAREL++KY IG L Sbjct: 64 HSKDARELMEKYFIGEL 80 >At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana] Length = 134 Score = 91.9 bits (218), Expect = 4e-19 Identities = 40/75 (53%), Positives = 51/75 (68%) Frame = +1 Query: 211 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 390 K L+ +EV KH K W+II VYDVT F+++HPGG + LL GKD T FEDVGHS Sbjct: 6 KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65 Query: 391 DDARELLKKYKIGTL 435 D AR+++ KY IG + Sbjct: 66 DTARDMMDKYFIGEI 80 >At2g46650.1 68415.m05820 cytochrome b5, putative similar to cytochome b5 GI:2695711 from [Olea europaea] Length = 132 Score = 90.6 bits (215), Expect = 9e-19 Identities = 39/75 (52%), Positives = 53/75 (70%) Frame = +1 Query: 205 MSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVG 384 M+ ++ +V KHK + WI+IH VYD++ F++EHPGG + LL V GKD + FEDV Sbjct: 1 MANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVN 60 Query: 385 HSDDARELLKKYKIG 429 HS DA+EL+KKY IG Sbjct: 61 HSKDAKELMKKYCIG 75 >At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5 [Arabidopsis thaliana] GI:4240122; strong similarity to Cytochrome B5 SP:P49098 from [Nicotiana tabacum] Length = 140 Score = 88.2 bits (209), Expect = 5e-18 Identities = 38/75 (50%), Positives = 49/75 (65%) Frame = +1 Query: 211 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 390 K TL EV +H K WI+I VYDVTKFL++HPGG + +L GKD T FEDVGHS Sbjct: 6 KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65 Query: 391 DDARELLKKYKIGTL 435 A+ +L +Y +G + Sbjct: 66 STAKAMLDEYYVGDI 80 >At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1) (NR1){Arabidopsis thaliana} Length = 917 Score = 87.8 bits (208), Expect = 6e-18 Identities = 39/76 (51%), Positives = 54/76 (71%) Frame = +1 Query: 208 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 387 SK ++ EV+KH S WII+H +YD T+FL++HPGG DS+L AG D T+ FE + H Sbjct: 545 SKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-H 603 Query: 388 SDDARELLKKYKIGTL 435 SD A++LL+ Y+IG L Sbjct: 604 SDKAKKLLEDYRIGEL 619 >At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1) (NR2) {Arabidopsis thaliana} Length = 917 Score = 86.2 bits (204), Expect = 2e-17 Identities = 38/76 (50%), Positives = 54/76 (71%) Frame = +1 Query: 208 SKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGH 387 +K ++ EVKKH S WII+H +YD T+FL +HPGG+DS+L AG D T+ FE + H Sbjct: 542 AKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-H 600 Query: 388 SDDARELLKKYKIGTL 435 SD A+++L+ Y+IG L Sbjct: 601 SDKAKKMLEDYRIGEL 616 >At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein contains InterPro accession IPR001199: Cytochrome b5 Length = 121 Score = 62.9 bits (146), Expect = 2e-10 Identities = 29/75 (38%), Positives = 44/75 (58%) Frame = +1 Query: 211 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHS 390 K+ + EV H WIII + VYD+T ++EEHPGG D++L+ AG D T F H+ Sbjct: 47 KSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHA 105 Query: 391 DDARELLKKYKIGTL 435 ++++ + IG L Sbjct: 106 TRVFDMIEDFYIGEL 120 >At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803 Length = 211 Score = 61.3 bits (142), Expect = 6e-10 Identities = 26/73 (35%), Positives = 43/73 (58%) Frame = +1 Query: 217 LTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAFEDVGHSDD 396 + +DEVKKH+ S+W ++ VY+++ ++ HPGG D L++ G+DGT F + Sbjct: 129 IPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVN 188 Query: 397 ARELLKKYKIGTL 435 LL+K +G L Sbjct: 189 VDILLEKCLVGVL 201 >At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative similar to delta-8 sphingolipid desaturase GI:3819708 from [Brassica napus] Length = 449 Score = 54.4 bits (125), Expect = 7e-08 Identities = 22/52 (42%), Positives = 35/52 (67%) Frame = +1 Query: 217 LTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 372 +T +++KKH +WI I VYDV+ +++ HPGG ++L +AG+D T AF Sbjct: 10 VTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61 >At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) identical to delta-8 sphingolipid desaturase GI:3819710 from [Arabidopsis thaliana]; contains Pfam profile PF00487: Fatty acid desaturase; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 449 Score = 51.6 bits (118), Expect = 5e-07 Identities = 21/54 (38%), Positives = 34/54 (62%) Frame = +1 Query: 211 KNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGKDGTQAF 372 K +T +++KKH +WI I VY+V+ +++ HPGG +L + G+D T AF Sbjct: 8 KYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61 >At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor family protein low similarity to extensin [Volvox carteri] GI:21992 Length = 312 Score = 33.1 bits (72), Expect = 0.18 Identities = 23/58 (39%), Positives = 29/58 (50%) Frame = -3 Query: 479 NFEQSVMILHLSPGGNVPILYFFNNSRASSLCPTSSKAWVPSFPATSNNESAPPGCSS 306 N ILHLS + IL F S +SSL P+SS + P +S+ SAPP S Sbjct: 2 NILSQTQILHLS----IAILLFITTS-SSSLSPSSSSPSLSPSPPSSSPSSAPPSSLS 54 >At2g32350.1 68415.m03954 ubiquitin family protein contains INTERPRO:IPR000626 ubiquitin domain Length = 233 Score = 29.5 bits (63), Expect = 2.3 Identities = 15/33 (45%), Positives = 22/33 (66%) Frame = +1 Query: 559 KRNTFYANPIDLKSDYQDVNEYGTILERFEILL 657 KR Y + I+L DY+++NEYG I E EI++ Sbjct: 112 KRMQLYYSGIELADDYRNLNEYG-ITEFSEIVV 143 >At4g09440.1 68417.m01554 hypothetical protein contains Pfam PF04510 : Family of unknown function (DUF577); common family comprised of At4g09440, At1g66000, At1g66060 Length = 177 Score = 28.3 bits (60), Expect = 5.2 Identities = 13/47 (27%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = +1 Query: 250 EKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVAGK--DGTQAFEDVG 384 + S W++ + T L E P A+S+ E+A K D + ++G Sbjct: 103 DNSCWVLAFTGAFCATIHLREFPSQAESVKEIANKMIDSVRELVEIG 149 >At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis thaliana]; identical to cDNA SMT3 protein GI:1707371 Length = 100 Score = 27.5 bits (58), Expect = 9.2 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +1 Query: 319 GGADSLLEVAGKDGTQAFEDVGHSDDARELLKKY 420 GGA L+V G+DG + F + S ++L+ Y Sbjct: 14 GGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAY 47 >At3g44820.1 68416.m04829 phototropic-responsive NPH3 family protein contains NPH3 family domain, Pfam:PF03000 Length = 661 Score = 27.5 bits (58), Expect = 9.2 Identities = 19/49 (38%), Positives = 24/49 (48%) Frame = +1 Query: 202 KMSKNLTLDEVKKHKDEKSVWIIIHNDVYDVTKFLEEHPGGADSLLEVA 348 K K + E K D++S+W T LEE PGGAD+ L VA Sbjct: 89 KCGKLARMYEDSKSTDKQSLW----------TTVLEEFPGGADNFLIVA 127 >At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50) identical to DNA repair-recombination protein GI:7110148 from [Arabidopsis thaliana] Length = 1316 Score = 27.5 bits (58), Expect = 9.2 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -2 Query: 525 DPYEQGSSQRHHCPFQFRTI-CDDFASFTWRQR 430 +P+E+ + Q H CP R+ D+ ASF +QR Sbjct: 683 EPFEKRARQEHSCPCCERSFTADEEASFIKKQR 715 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,827,979 Number of Sequences: 28952 Number of extensions: 272584 Number of successful extensions: 817 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 792 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 815 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1516419560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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