BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc14b17
(617 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 35 6e-04
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 35 7e-04
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 31 0.009
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 25 0.45
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 24 1.0
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 24 1.0
DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 23 3.2
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 22 4.2
AY569702-1|AAS86655.1| 400|Apis mellifera feminizer protein. 22 4.2
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 22 5.5
AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor pr... 22 5.5
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 22 5.5
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 21 7.3
AY331183-1|AAP94623.1| 953|Apis mellifera NMDA-type glutamate r... 21 7.3
DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 21 9.6
AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 21 9.6
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 35.1 bits (77), Expect = 6e-04
Identities = 34/118 (28%), Positives = 55/118 (46%)
Frame = +3
Query: 138 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKS 317
M K++ N+ VI HVD GKST T L+ K G I R E +F + E + + K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGI-DKRTIE-KF-EKEAQEMGKG-SFKY 56
Query: 318 TAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 491
+ + E + + I + ++ K + + +ID+PGH DF + D A+
Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETSK-YYVTIIDAPGHRDFIKNMITGTSQADCAV 113
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 34.7 bits (76), Expect = 7e-04
Identities = 34/118 (28%), Positives = 55/118 (46%)
Frame = +3
Query: 138 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKS 317
M K++ N+ VI HVD GKST T L+ K G I R E +F + E + + K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGI-DKRTIE-KF-EKEAQEMGKG-SFKY 56
Query: 318 TAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 491
+ + E + + I + ++ K + + +ID+PGH DF + D A+
Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETAK-YYVTIIDAPGHRDFIKNMITGTSQADCAV 113
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 31.1 bits (67), Expect = 0.009
Identities = 17/73 (23%), Positives = 32/73 (43%)
Frame = +3
Query: 396 EKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAERIKPI 575
E G + +D+PGH F S +TD + QT + A ++ I
Sbjct: 190 ESGERVTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKEQTLQSIEMAKDAKVPII 249
Query: 576 LFMNKMDRALLEL 614
+ +NK+D+ +++
Sbjct: 250 VAINKIDKPNIDI 262
Score = 29.5 bits (63), Expect = 0.027
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = +3
Query: 165 MSVIAHVDHGKSTLTDSL 218
++++ HVDHGK+TL D+L
Sbjct: 148 VTIMGHVDHGKTTLLDAL 165
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 25.4 bits (53), Expect = 0.45
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3
Query: 162 NMSVIAHVDHGKSTLTDSL 218
N+ I HV HGKST+ ++
Sbjct: 44 NIGTIGHVAHGKSTIVKAI 62
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 24.2 bits (50), Expect = 1.0
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +3
Query: 405 FLINLIDSPGHVDFSSEVTAALRVTDGAL 491
+ + +ID+PGH DF + D A+
Sbjct: 12 YYVTIIDAPGHRDFIKNMITGTSQADCAV 40
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 24.2 bits (50), Expect = 1.0
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +3
Query: 405 FLINLIDSPGHVDFSSEVTAALRVTDGAL 491
+ + +ID+PGH DF + D A+
Sbjct: 28 YYVTIIDAPGHRDFIKNMITGTSQADCAV 56
>DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine
receptor beta1subunit protein.
Length = 520
Score = 22.6 bits (46), Expect = 3.2
Identities = 8/21 (38%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Frame = +1
Query: 28 PFSKRTPLLAYGSW-FNRTKI 87
PF ++T ++ +GSW FN ++
Sbjct: 159 PFDQQTCIMKFGSWTFNGDQV 179
Score = 22.2 bits (45), Expect = 4.2
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = -1
Query: 143 VHHPTDLVYREIHH 102
+HHP + R++HH
Sbjct: 400 LHHPNCKINRKVHH 413
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 22.2 bits (45), Expect = 4.2
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +1
Query: 28 PFSKRTPLLAYGSW 69
PF ++T +L +GSW
Sbjct: 161 PFDEQTCVLKFGSW 174
>AY569702-1|AAS86655.1| 400|Apis mellifera feminizer protein.
Length = 400
Score = 22.2 bits (45), Expect = 4.2
Identities = 13/44 (29%), Positives = 18/44 (40%)
Frame = -1
Query: 545 TQYCFSLYTHTRHTVNNHKGSISDTECSCYFRREINVSR*VNQV 414
T C S Y +RH N + + CS RE R +Q+
Sbjct: 227 TSSCHSRYEDSRHEDRNSYRNDGERSCSRDRSREYKKDRRYDQL 270
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 21.8 bits (44), Expect = 5.5
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 324 ISMFFELEEKDLVFITNPDQREKSEKGFLI 413
++MF+ D+ I+N +Q + S GF I
Sbjct: 527 LNMFYNNFNSDIKSISNNEQVKVSALGFFI 556
>AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor
protein.
Length = 501
Score = 21.8 bits (44), Expect = 5.5
Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = -2
Query: 508 TQSTTTRAP-SVTRSAAVTSEEKSTC 434
+ STTT +P + T+S V + STC
Sbjct: 324 SSSTTTTSPMTSTKSTIVRNHLNSTC 349
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 21.8 bits (44), Expect = 5.5
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 324 ISMFFELEEKDLVFITNPDQREKSEKGFLI 413
++MF+ D+ I+N +Q + S GF I
Sbjct: 527 LNMFYNNFNSDIKSISNNEQVKVSALGFFI 556
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 21.4 bits (43), Expect = 7.3
Identities = 6/14 (42%), Positives = 11/14 (78%)
Frame = +1
Query: 28 PFSKRTPLLAYGSW 69
PF ++T ++ +GSW
Sbjct: 157 PFDEQTCVMKFGSW 170
>AY331183-1|AAP94623.1| 953|Apis mellifera NMDA-type glutamate
receptor 1 protein.
Length = 953
Score = 21.4 bits (43), Expect = 7.3
Identities = 6/13 (46%), Positives = 10/13 (76%)
Frame = +3
Query: 300 CITIKSTAISMFF 338
C T+K +A+ M+F
Sbjct: 688 CATVKGSAVDMYF 700
>DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine
receptor alpha1subunit protein.
Length = 601
Score = 21.0 bits (42), Expect = 9.6
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +1
Query: 28 PFSKRTPLLAYGSW 69
PF ++T + +GSW
Sbjct: 153 PFDEQTCFMKFGSW 166
>AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic
acetylcholine receptorApisa2 subunit protein.
Length = 541
Score = 21.0 bits (42), Expect = 9.6
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +1
Query: 28 PFSKRTPLLAYGSW 69
PF ++T + +GSW
Sbjct: 153 PFDQQTCFMKFGSW 166
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 168,836
Number of Sequences: 438
Number of extensions: 3657
Number of successful extensions: 26
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18337950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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