Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_C01
(911 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 182 8e-47
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 181 1e-46
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 181 1e-46
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 105 7e-24
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 102 8e-23
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 71 2e-13
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 48 1e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 3e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.024
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 34 0.032
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 31 0.23
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.40
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 30 0.52
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.69
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 28 2.1
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 28 2.1
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 28 2.1
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 2.8
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 27 3.7
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 26 6.5
SPAC1B3.16c |vht1||vitamin H transporter Vth1|Schizosaccharomyce... 26 8.5
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 182 bits (442), Expect = 8e-47
Identities = 84/94 (89%), Positives = 88/94 (93%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K FKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQAD
Sbjct: 51 KGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
CAVLI+ GTGEFEAGISK+GQTREHALLA+TLG
Sbjct: 111 CAVLIIGGGTGEFEAGISKDGQTREHALLAYTLG 144
Score = 78.2 bits (184), Expect = 2e-15
Identities = 38/54 (70%), Positives = 42/54 (77%)
Frame = +3
Query: 114 MGKVKDSH*HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
MGK K H + + +DSGKSTTTGH IYKCGGIDKR IEKFEKEA E+GKGS
Sbjct: 1 MGKEK-GHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 31.9 bits (69), Expect = 0.13
Identities = 23/46 (50%), Positives = 29/46 (63%), Gaps = 1/46 (2%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*G-NQEGSILIHLXKIGYNPXAV 685
VKQLIV VNKMD+T +S+ RF +E S I K+G+NP V
Sbjct: 145 VKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI--KKVGFNPKTV 186
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 181 bits (441), Expect = 1e-46
Identities = 83/94 (88%), Positives = 88/94 (93%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K FKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQAD
Sbjct: 51 KGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
CA+LI+ GTGEFEAGISK+GQTREHALLA+TLG
Sbjct: 111 CAILIIGGGTGEFEAGISKDGQTREHALLAYTLG 144
Score = 78.2 bits (184), Expect = 2e-15
Identities = 38/54 (70%), Positives = 42/54 (77%)
Frame = +3
Query: 114 MGKVKDSH*HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
MGK K H + + +DSGKSTTTGH IYKCGGIDKR IEKFEKEA E+GKGS
Sbjct: 1 MGKEK-GHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 31.9 bits (69), Expect = 0.13
Identities = 23/46 (50%), Positives = 29/46 (63%), Gaps = 1/46 (2%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*G-NQEGSILIHLXKIGYNPXAV 685
VKQLIV VNKMD+T +S+ RF +E S I K+G+NP V
Sbjct: 145 VKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI--KKVGFNPKTV 186
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 181 bits (441), Expect = 1e-46
Identities = 83/94 (88%), Positives = 88/94 (93%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K FKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQAD
Sbjct: 51 KGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQAD 110
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
CA+LI+ GTGEFEAGISK+GQTREHALLA+TLG
Sbjct: 111 CAILIIGGGTGEFEAGISKDGQTREHALLAYTLG 144
Score = 78.2 bits (184), Expect = 2e-15
Identities = 38/54 (70%), Positives = 42/54 (77%)
Frame = +3
Query: 114 MGKVKDSH*HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
MGK K H + + +DSGKSTTTGH IYKCGGIDKR IEKFEKEA E+GKGS
Sbjct: 1 MGKEK-GHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 31.9 bits (69), Expect = 0.13
Identities = 23/46 (50%), Positives = 29/46 (63%), Gaps = 1/46 (2%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*G-NQEGSILIHLXKIGYNPXAV 685
VKQLIV VNKMD+T +S+ RF +E S I K+G+NP V
Sbjct: 145 VKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI--KKVGFNPKTV 186
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 105 bits (253), Expect = 7e-24
Identities = 51/94 (54%), Positives = 60/94 (63%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K F YAW+LD + ER RG+T+D+A FE+ K I DAPGHRDFI MI G S AD
Sbjct: 221 KGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSAD 280
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
AVL+V + FE G +NGQTREHA L LG
Sbjct: 281 FAVLVVDSSQNNFERGFLENGQTREHAYLLRALG 314
Score = 41.9 bits (94), Expect = 1e-04
Identities = 18/45 (40%), Positives = 28/45 (62%)
Frame = +3
Query: 141 HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
H ++ +DSGKST G +++ G I+ R+++K EA GKGS
Sbjct: 179 HLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGS 223
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 102 bits (244), Expect = 8e-23
Identities = 45/94 (47%), Positives = 63/94 (67%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K+ + +W LD ERE+G T+++ FET +++DAPGH+ ++ NMI G SQAD
Sbjct: 282 KESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQAD 341
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
VL+++A GEFEAG + GQTREHA+LA T G
Sbjct: 342 IGVLVISARRGEFEAGFERGGQTREHAVLARTQG 375
Score = 46.4 bits (105), Expect = 6e-06
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +3
Query: 162 IDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGSXQICLGIGQT 305
+D+GKST G+ ++ G +DKR +EK E+EA+E GK S + + T
Sbjct: 247 VDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDST 294
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 70.9 bits (166), Expect = 2e-13
Identities = 37/85 (43%), Positives = 52/85 (61%)
Frame = +1
Query: 295 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 474
+DK E+ RGITI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A
Sbjct: 91 IDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSAT 150
Query: 475 TGEFEAGISKNGQTREHALLAFTLG 549
G+ QTREH LLA +G
Sbjct: 151 DGQMP-------QTREHLLLARQVG 168
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 48.4 bits (110), Expect = 1e-06
Identities = 25/63 (39%), Positives = 33/63 (52%)
Frame = +1
Query: 292 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 471
V+D L AER+RGITI+ A F + +ID PGH DF + + D AV I+
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 472 GTG 480
G
Sbjct: 127 SAG 129
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 44.0 bits (99), Expect = 3e-05
Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = +1
Query: 295 LDKLKAERERGITIDIA----LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 462
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF ++ + + +L+
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 463 VAAGTG 480
V A G
Sbjct: 155 VDASQG 160
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.024
Identities = 31/96 (32%), Positives = 44/96 (45%), Gaps = 19/96 (19%)
Frame = +1
Query: 295 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHRDFIK 417
+D ++ ERE+GITI A W+ FE S Y + IID PGH DF
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDFTI 160
Query: 418 NMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 525
+ D AVL++ A +G I+ + Q R +
Sbjct: 161 EVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.9 bits (74), Expect = 0.032
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 325 GITIDIALWK--FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 480
GIT I + F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.1 bits (67), Expect = 0.23
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 373 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 468
+V+ +D PGH + M+ G + D A+L++A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.40
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +1
Query: 370 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 480
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 29.9 bits (64), Expect = 0.52
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +1
Query: 298 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 420
+K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.69
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 382 IIDAPGHRDFIKNMITGTSQADCAVLIV 465
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 370 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 465
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
Cho2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 905
Score = 27.9 bits (59), Expect = 2.1
Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 1/71 (1%)
Frame = -3
Query: 582 ILFTPTMSCLTSEGESEQGMLTGLTVLRDTSFE-FTGTGSYDEHSAISLRGSCDHVLDEI 406
+L P C T+ E GL L D + + +T T S SAI G H
Sbjct: 703 LLGHPITVCWTASPNHEINDWIGLYKLSDNASDLYTQTSSEGRWSAIDANGYTSHCSSIK 762
Query: 405 SVSRSINDGNI 373
S+S N G +
Sbjct: 763 SLSNDKNSGEV 773
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 370 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 465
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 2.8
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +1
Query: 313 ERERGITID-----IALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 465
ERER ++I +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 185 ERERVMSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.1 bits (57), Expect = 3.7
Identities = 13/60 (21%), Positives = 26/60 (43%)
Frame = -3
Query: 279 EXILYPFPGPPSRTSRWHVCQYHHICRSXDQWWWTCRSRSCPIDDNVNVSLLPCPFLVSH 100
E + Y G + S W H+ ++ + + + P DD + ++L PF ++H
Sbjct: 141 ESLKYRLQGVTTDPSLWGHEYVRHLASEIEEEFASRQEEEAPTDDLMELALTIVPFFLTH 200
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +2
Query: 476 PVNSKLVSLRTVKPVSMPCSLSPSD 550
P ++ SL T KPVSMP S SD
Sbjct: 392 PPPPRVKSLATNKPVSMPVSTEQSD 416
>SPAC1B3.16c |vht1||vitamin H transporter Vth1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 568
Score = 25.8 bits (54), Expect = 8.5
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = +3
Query: 702 FXGWPGDNMLXPSNQKCPXVXGMA 773
+ GWPGD SN C + G++
Sbjct: 495 YSGWPGDPTFRKSNDVCIALIGVS 518
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,357,210
Number of Sequences: 5004
Number of extensions: 66297
Number of successful extensions: 174
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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