Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP22_F_C01
(911 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 194 7e-52
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 191 9e-51
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 184 8e-49
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 151 6e-39
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.004
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.9
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 5.1
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 194 bits (474), Expect = 7e-52
Identities = 92/94 (97%), Positives = 92/94 (97%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K FKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD
Sbjct: 51 KGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 110
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG
Sbjct: 111 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 144
Score = 81.0 bits (191), Expect = 1e-17
Identities = 40/54 (74%), Positives = 43/54 (79%)
Frame = +3
Query: 114 MGKVKDSH*HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
MGK K H + + +DSGKSTTTGH IYKCGGIDKR IEKFEKEAQEMGKGS
Sbjct: 1 MGKEK-IHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 53
Score = 60.5 bits (140), Expect = 2e-11
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*GNQEGSILIHLXKIGYNPXAVS 688
VKQLIVGVNKMDSTEPPYSE RF + + ++ KIGYNP AV+
Sbjct: 145 VKQLIVGVNKMDSTEPPYSETRF-EEIKKEVSSYIKKIGYNPAAVA 189
Score = 38.3 bits (85), Expect = 1e-04
Identities = 24/50 (48%), Positives = 28/50 (56%)
Frame = +3
Query: 618 FEEIKKEVSSYISRRLATTXLXCLFXXXFXGWPGDNMLXPSNQKCPXVXG 767
FEEIKKEVSSYI +++ F GW GDNML S+ K P G
Sbjct: 167 FEEIKKEVSSYI-KKIGYNPAAVAFVP-ISGWHGDNMLEVSS-KMPWFKG 213
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 191 bits (465), Expect = 9e-51
Identities = 90/94 (95%), Positives = 91/94 (96%)
Frame = +1
Query: 268 KDPFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQAD 447
K FKYAWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQAD
Sbjct: 51 KGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQAD 110
Query: 448 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 549
CAVLIVAAG GEFEAGISKNGQTREHALLAFTLG
Sbjct: 111 CAVLIVAAGIGEFEAGISKNGQTREHALLAFTLG 144
Score = 81.0 bits (191), Expect = 1e-17
Identities = 40/54 (74%), Positives = 43/54 (79%)
Frame = +3
Query: 114 MGKVKDSH*HCRLSDTIDSGKSTTTGHXIYKCGGIDKRAIEKFEKEAQEMGKGS 275
MGK K H + + +DSGKSTTTGH IYKCGGIDKR IEKFEKEAQEMGKGS
Sbjct: 1 MGKEK-IHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 53
Score = 52.8 bits (121), Expect = 4e-09
Identities = 26/46 (56%), Positives = 33/46 (71%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*GNQEGSILIHLXKIGYNPXAVS 688
VKQLIVGVNKMD T+PPYSE RF + + ++ KIGYN +V+
Sbjct: 145 VKQLIVGVNKMDMTDPPYSEARF-EEIKKEVSSYIKKIGYNTASVA 189
Score = 41.1 bits (92), Expect = 1e-05
Identities = 25/50 (50%), Positives = 28/50 (56%)
Frame = +3
Query: 618 FEEIKKEVSSYISRRLATTXLXCLFXXXFXGWPGDNMLXPSNQKCPXVXG 767
FEEIKKEVSSYI +++ F GW GDNML PS K P G
Sbjct: 167 FEEIKKEVSSYI-KKIGYNTASVAFVP-ISGWHGDNMLEPS-PKTPWYKG 213
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 184 bits (449), Expect = 8e-49
Identities = 87/87 (100%), Positives = 87/87 (100%)
Frame = +1
Query: 289 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 468
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 469 AGTGEFEAGISKNGQTREHALLAFTLG 549
AGTGEFEAGISKNGQTREHALLAFTLG
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLG 87
Score = 60.5 bits (140), Expect = 2e-11
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*GNQEGSILIHLXKIGYNPXAVS 688
VKQLIVGVNKMDSTEPPYSE RF + + ++ KIGYNP AV+
Sbjct: 88 VKQLIVGVNKMDSTEPPYSETRF-EEIKKEVSSYIKKIGYNPAAVA 132
Score = 38.3 bits (85), Expect = 1e-04
Identities = 24/50 (48%), Positives = 28/50 (56%)
Frame = +3
Query: 618 FEEIKKEVSSYISRRLATTXLXCLFXXXFXGWPGDNMLXPSNQKCPXVXG 767
FEEIKKEVSSYI +++ F GW GDNML S+ K P G
Sbjct: 110 FEEIKKEVSSYI-KKIGYNPAAVAFVP-ISGWHGDNMLEVSS-KMPWFKG 156
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 151 bits (367), Expect = 6e-39
Identities = 71/71 (100%), Positives = 71/71 (100%)
Frame = +1
Query: 337 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 516
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 517 REHALLAFTLG 549
REHALLAFTLG
Sbjct: 61 REHALLAFTLG 71
Score = 60.5 bits (140), Expect = 2e-11
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = +2
Query: 551 VKQLIVGVNKMDSTEPPYSEPRF*GNQEGSILIHLXKIGYNPXAVS 688
VKQLIVGVNKMDSTEPPYSE RF + + ++ KIGYNP AV+
Sbjct: 72 VKQLIVGVNKMDSTEPPYSETRF-EEIKKEVSSYIKKIGYNPAAVA 116
Score = 38.3 bits (85), Expect = 1e-04
Identities = 24/50 (48%), Positives = 28/50 (56%)
Frame = +3
Query: 618 FEEIKKEVSSYISRRLATTXLXCLFXXXFXGWPGDNMLXPSNQKCPXVXG 767
FEEIKKEVSSYI +++ F GW GDNML S+ K P G
Sbjct: 94 FEEIKKEVSSYI-KKIGYNPAAVAFVP-ISGWHGDNMLEVSS-KMPWFKG 140
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.004
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +1
Query: 376 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 480
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.9
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 361 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 462
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 5.1
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 361 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 462
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 239,468
Number of Sequences: 438
Number of extensions: 4717
Number of successful extensions: 28
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21
length of database: 146,343
effective HSP length: 58
effective length of database: 120,939
effective search space used: 29630055
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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