Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_G19
(900 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 32 0.097
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 30 0.39
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.68
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 29 0.68
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 29 0.90
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.90
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 29 1.2
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 3.6
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 3.6
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 4.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.8
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.8
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 6.3
SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces... 26 6.3
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.3
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 6.3
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 8.4
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 26 8.4
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.4
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 8.4
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 32.3 bits (70), Expect = 0.097
Identities = 24/88 (27%), Positives = 41/88 (46%)
Frame = +1
Query: 391 EALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQET 570
EALE+ +Q +E +E K ++ +L E+ V+N +E ++ K +
Sbjct: 1362 EALEKEKQELETKLQETAKETDTFKQQVNSLNEE----VENLKKEVEQANTKNTRLAAAW 1417
Query: 571 NEKLAPKIKAAYDDFAKNTQEVIKKIQE 654
NEK K++ FA QE+ K +E
Sbjct: 1418 NEKCENLKKSSLTRFAHLKQELTNKNKE 1445
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 30.3 bits (65), Expect = 0.39
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%)
Frame = +1
Query: 382 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 537
K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186
Query: 538 AKKVSSNVQETNEKLAPKIK 597
K + T +KL KI+
Sbjct: 187 YKDMEQENASTIQKLTSKIR 206
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 29.5 bits (63), Expect = 0.68
Identities = 14/65 (21%), Positives = 31/65 (47%)
Frame = +1
Query: 382 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 561
KA + LE+ ++ E + EE+ H + T+ + + + +QE ++ K +
Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEKSRVDKMIDEYR 412
Query: 562 QETNE 576
Q+ +E
Sbjct: 413 QKLSE 417
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 29.5 bits (63), Expect = 0.68
Identities = 17/61 (27%), Positives = 33/61 (54%), Gaps = 3/61 (4%)
Frame = +1
Query: 364 LGDANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQK 534
+ +A KA+++LEQ+ + E L K H + E+ + +EKL A ++ + S++
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111
Query: 535 L 537
L
Sbjct: 112 L 112
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 29.1 bits (62), Expect = 0.90
Identities = 21/88 (23%), Positives = 46/88 (52%)
Frame = +1
Query: 397 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 576
++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E
Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553
Query: 577 KLAPKIKAAYDDFAKNTQEVIKKIQEAA 660
L+ K + DD+ +EV+ K++ A
Sbjct: 554 VLSKSSKES-DDY----EEVVGKLRTEA 576
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.90
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +1
Query: 382 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 561
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 562 QETNEKLAP 588
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 28.7 bits (61), Expect = 1.2
Identities = 26/111 (23%), Positives = 52/111 (46%), Gaps = 6/111 (5%)
Frame = +1
Query: 382 KAKEALEQSRQNIERTAEELRKAHPDV---EKNATALREKLQ--AAVQNTVQESQKLAKK 546
K +E ++ +NI+ E+LRK+ + + A LRE + T++ +
Sbjct: 679 KKQEDVDSLEKNIQTLKEDLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHS 738
Query: 547 VSSNVQETNEKLAPKIKAAYDDFAKNTQEVIKKIQEAANAKQ-*ASILNSH 696
S+ + TN L+ ++ + +D + T V Q++ KQ S++NS+
Sbjct: 739 SLSDAKNTNAILSSELTKSSEDVKRLTANVETLTQDSKAMKQSFTSLVNSY 789
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 27.1 bits (57), Expect = 3.6
Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +1
Query: 385 AKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 489
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 157 ASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 27.1 bits (57), Expect = 3.6
Identities = 21/84 (25%), Positives = 40/84 (47%)
Frame = +1
Query: 382 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 561
KAKE ++ R +RTA E+RK +E+ R + A Q + Q +++ +
Sbjct: 164 KAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRAERAAEAQRVAGKEQ-----LANIL 218
Query: 562 QETNEKLAPKIKAAYDDFAKNTQE 633
+ + + L +I+ A + + T E
Sbjct: 219 KHSTDLLEARIERANINISAQTSE 242
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 435 LGRALDVLPRLFQSLLGLAVRVSERXPGDSW 343
+G + L RLF SL GL ++ PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +3
Query: 180 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 299
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3
Query: 174 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 263
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 26.2 bits (55), Expect = 6.3
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = +1
Query: 412 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 591
+NI + E+ R + NA+ + K Q + T++ Q+LAK + +++ E+L+
Sbjct: 136 KNILKAIEDSRYPFVANKLNASTIEVKPQ---RTTLESRQQLAKVLEGYAKDSREQLSAM 192
Query: 592 IKAAYDDFAKN 624
+ AKN
Sbjct: 193 RTELKKEIAKN 203
>SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 172
Score = 26.2 bits (55), Expect = 6.3
Identities = 13/86 (15%), Positives = 41/86 (47%)
Frame = +1
Query: 388 KEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQE 567
K ++R+N++ + + P+++ + + +L+ +++++ Q + + V+E
Sbjct: 81 KRRNRKARENLKVNVSSIGEVLPEIDLDISVANSRLKPVIKDSLSSKQTKSSMKRNTVEE 140
Query: 568 TNEKLAPKIKAAYDDFAKNTQEVIKK 645
E+ +K + F N E +++
Sbjct: 141 I-ERFQAILK--HPSFVSNPLETVRE 163
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.3
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +3
Query: 87 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 266
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 267 SKDA 278
SKDA
Sbjct: 230 SKDA 233
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +1
Query: 397 LEQSRQNIERTAEELRKAHPDVEK 468
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +1
Query: 544 KVSSNVQET--NEKLAPKIKAAYD 609
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC23H4.11c |cnl2||centromere localized protein
Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 8.4
Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +1
Query: 388 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 561
++ L + R NI ++ + K+ D + N L+ A+ + V+E ++
Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112
Query: 562 QETNEKLAPKIKAAY 606
E NE+L+ +++AY
Sbjct: 113 SEINERLSEAVQSAY 127
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.4
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +3
Query: 144 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 320
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 321 LQQL 332
Q+
Sbjct: 73 NNQI 76
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -3
Query: 412 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 290
AST+ K PWP + L +P + + CS+ +E ++ +
Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,260,005
Number of Sequences: 5004
Number of extensions: 36191
Number of successful extensions: 189
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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