Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_D01
(903 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein. 99 6e-23
EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein. 99 6e-23
EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein. 87 2e-19
AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein. 87 2e-19
EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein. 85 8e-19
EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein. 85 8e-19
EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage prot... 60 2e-11
AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase prot... 37 3e-04
DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 25 0.71
DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 25 0.71
DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chlor... 24 2.2
DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chlor... 24 2.2
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 22 8.8
AB208108-1|BAE72140.1| 92|Apis mellifera Broad complex zinc fi... 22 8.8
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 22 8.8
>EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein.
Length = 684
Score = 98.7 bits (235), Expect = 6e-23
Identities = 49/121 (40%), Positives = 73/121 (60%), Gaps = 1/121 (0%)
Frame = +1
Query: 136 FKTTPVDAAFVEKQKKILSLFYNVNEINY-EAEYYKVAQDFNIEASKDCYTNMKAYENFM 312
+ T D F+ KQKK+ +L Y V + +Y Q +NIEA+ D YTN A + F+
Sbjct: 24 YDTKTADKDFLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFL 83
Query: 313 MMYKVGFLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVLIR 492
+YK G LP+ FS++Y ++ E ALFKLFY+AKDF+ F+KTA +A+ +N+ +
Sbjct: 84 SIYKHGMLPRGELFSLYYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYS 143
Query: 493 L 495
L
Sbjct: 144 L 144
Score = 68.5 bits (160), Expect = 8e-14
Identities = 38/112 (33%), Positives = 60/112 (53%), Gaps = 2/112 (1%)
Frame = +2
Query: 482 FLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYGI 661
++Y+ Y A+I R DT LP YE P +F EV K ++ ++ G LD K Y
Sbjct: 140 YIYSLYTAVITRPDTKFIQLPPLYEMCPYFFFNSEVLQKANHA-LIFGKLDTKTSGKY-- 196
Query: 662 XKENEQFVMYANYSN-SLTYPNN-EDRIAYLTEDVGLXAYYYYFHSHLPFWV 811
+++++ ANYS L + N E+++ Y ED+GL YY++ PFW+
Sbjct: 197 ----KEYIIPANYSGWYLNHDYNLENKLNYFIEDIGLNTYYFFLRQAFPFWL 244
>EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein.
Length = 684
Score = 98.7 bits (235), Expect = 6e-23
Identities = 49/121 (40%), Positives = 73/121 (60%), Gaps = 1/121 (0%)
Frame = +1
Query: 136 FKTTPVDAAFVEKQKKILSLFYNVNEINY-EAEYYKVAQDFNIEASKDCYTNMKAYENFM 312
+ T D F+ KQKK+ +L Y V + +Y Q +NIEA+ D YTN A + F+
Sbjct: 24 YDTKTADKDFLLKQKKVYNLLYRVAQPALANITWYNEGQAWNIEANIDSYTNAAAVKEFL 83
Query: 313 MMYKVGFLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVLIR 492
+YK G LP+ FS++Y ++ E ALFKLFY+AKDF+ F+KTA +A+ +N+ +
Sbjct: 84 SIYKHGMLPRGELFSLYYPQLLREMSALFKLFYHAKDFDIFFKTALWAKNNINEAQYIYS 143
Query: 493 L 495
L
Sbjct: 144 L 144
Score = 68.9 bits (161), Expect = 6e-14
Identities = 38/112 (33%), Positives = 60/112 (53%), Gaps = 2/112 (1%)
Frame = +2
Query: 482 FLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYGI 661
++Y+ Y A+I R DT LP YE P +F EV K ++ ++ G LD K Y
Sbjct: 140 YIYSLYTAVITRPDTKFIQLPPLYEMCPYFFFNSEVLQKANHA-LIFGKLDTKTSGKY-- 196
Query: 662 XKENEQFVMYANYSN-SLTYPNN-EDRIAYLTEDVGLXAYYYYFHSHLPFWV 811
+++++ ANYS L + N E+++ Y ED+GL YY++ PFW+
Sbjct: 197 ----KEYIIPANYSGWYLNHDYNLENKLIYFIEDIGLNTYYFFLRQAFPFWL 244
>EF625896-1|ABR45903.1| 683|Apis mellifera hexamerin protein.
Length = 683
Score = 87.4 bits (207), Expect = 2e-19
Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 1/115 (0%)
Frame = +1
Query: 154 DAAFVEKQKKILSLFYNVNEIN-YEAEYYKVAQDFNIEASKDCYTNMKAYENFMMMYKVG 330
D +V +QK I LF++V++ Y E Y+ A+ FN+ + D Y + +A FM + K G
Sbjct: 28 DKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLLKHG 87
Query: 331 FLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVLIRL 495
LP+ F++ ++MR +A+ LF+L Y AK F+ FY TA +AR +N+ L L
Sbjct: 88 MLPRGQVFTMMNKEMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYAL 142
Score = 62.5 bits (145), Expect = 5e-12
Identities = 42/119 (35%), Positives = 59/119 (49%), Gaps = 3/119 (2%)
Frame = +2
Query: 479 MFLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYG 658
M+LYA +A+I R DT LP YE P + EV K + M D D K YN
Sbjct: 137 MYLYALSVAVIHRPDTKLMKLPPMYEVMPHLYFNDEVMQKAYNIAMGD-TADMKKTYN-- 193
Query: 659 IXKENEQFVMYANYSNSLTYPNN--EDRIAYLTEDVGLXAYYYYF-HSHLPFWVGLWLN 826
+ +++ ANY+ +N E R+ Y TEDVGL +Y+ H++ PF + LN
Sbjct: 194 ---NIDYYLLAANYTGWYLTKHNVPEQRLNYFTEDVGLNHFYFMLNHNYPPFMLSNSLN 249
>AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein.
Length = 683
Score = 87.4 bits (207), Expect = 2e-19
Identities = 43/115 (37%), Positives = 68/115 (59%), Gaps = 1/115 (0%)
Frame = +1
Query: 154 DAAFVEKQKKILSLFYNVNEIN-YEAEYYKVAQDFNIEASKDCYTNMKAYENFMMMYKVG 330
D +V +QK I LF++V++ Y E Y+ A+ FN+ + D Y + +A FM + K G
Sbjct: 28 DKTYVTRQKNIYELFWHVDQPTVYHPELYQKARTFNLVENLDNYNDKEAVNEFMQLLKHG 87
Query: 331 FLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVLIRL 495
LP+ F++ ++MR +A+ LF+L Y AK F+ FY TA +AR +N+ L L
Sbjct: 88 MLPRGQVFTMMNKEMRHQAVVLFRLLYSAKTFDVFYNTAVWARFNVNEQMYLYAL 142
Score = 62.5 bits (145), Expect = 5e-12
Identities = 42/119 (35%), Positives = 59/119 (49%), Gaps = 3/119 (2%)
Frame = +2
Query: 479 MFLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYG 658
M+LYA +A+I R DT LP YE P + EV K + M D D K YN
Sbjct: 137 MYLYALSVAVIHRPDTKLMKLPPMYEVMPHLYFNDEVMQKAYNIAMGD-TADMKKTYN-- 193
Query: 659 IXKENEQFVMYANYSNSLTYPNN--EDRIAYLTEDVGLXAYYYYF-HSHLPFWVGLWLN 826
+ +++ ANY+ +N E R+ Y TEDVGL +Y+ H++ PF + LN
Sbjct: 194 ---NIDYYLLAANYTGWYLTKHNVPEQRLNYFTEDVGLNHFYFMLNHNYPPFMLSNSLN 249
>EF625898-1|ABR45905.1| 686|Apis mellifera hexamerin protein.
Length = 686
Score = 85.0 bits (201), Expect = 8e-19
Identities = 43/110 (39%), Positives = 66/110 (60%), Gaps = 2/110 (1%)
Frame = +1
Query: 154 DAAFVEKQKKILSLFYNVNEINY-EAEYYKVAQDFNIEASKDCYTNMKAYENFMMMYKVG 330
D F+ KQKKI L V + + +AE+Y V +++++E++ D Y + + F+ YK G
Sbjct: 29 DMDFLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQG 88
Query: 331 -FLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQG 477
FL +N F+ + + E LF+L Y AKDF+ FYKTA +AR+ MN G
Sbjct: 89 MFLSRNAIFTPLNSEQKYEVRMLFELLYNAKDFQTFYKTAAWARLRMNSG 138
Score = 68.1 bits (159), Expect = 1e-13
Identities = 40/113 (35%), Positives = 54/113 (47%), Gaps = 2/113 (1%)
Frame = +2
Query: 479 MFLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYG 658
MF A+ IA++ R DT PA YE YP YF V + +KM G N
Sbjct: 139 MFTTAFSIAVLYRPDTKYMKFPAIYEIYPNYFFDSSVIEEAQNLKMSRGSSVVTGMNNI- 197
Query: 659 IXKENEQFVMYANYSNSL--TYPNNEDRIAYLTEDVGLXAYYYYFHSHLPFWV 811
E +++ NYS+ Y + E ++ Y EDV L AYYYY LP+W+
Sbjct: 198 -----ETYIVNTNYSSKYMREYNDPEYKLDYFMEDVELNAYYYYMREMLPYWM 245
>EF589162-1|ABQ84439.1| 686|Apis mellifera hexamerin 70c protein.
Length = 686
Score = 85.0 bits (201), Expect = 8e-19
Identities = 43/110 (39%), Positives = 66/110 (60%), Gaps = 2/110 (1%)
Frame = +1
Query: 154 DAAFVEKQKKILSLFYNVNEINY-EAEYYKVAQDFNIEASKDCYTNMKAYENFMMMYKVG 330
D F+ KQKKI L V + + +AE+Y V +++++E++ D Y + + F+ YK G
Sbjct: 29 DMDFLHKQKKIFDLLLYVRQADLSDAEWYDVGRNYDMESNMDMYKDKNVVQKFLWWYKQG 88
Query: 331 -FLPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQG 477
FL +N F+ + + E LF+L Y AKDF+ FYKTA +AR+ MN G
Sbjct: 89 MFLSRNAIFTPLNSEQKYEVRMLFELLYNAKDFQTFYKTAAWARLRMNSG 138
Score = 69.3 bits (162), Expect = 4e-14
Identities = 41/113 (36%), Positives = 54/113 (47%), Gaps = 2/113 (1%)
Frame = +2
Query: 479 MFLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYG 658
MF A+ IA++ R DT PA YE YP YF V + +KM G N
Sbjct: 139 MFTTAFSIAVLYRPDTKYMKFPAIYEIYPNYFFDSSVIEEAQNLKMSRGSSVVTGMNNI- 197
Query: 659 IXKENEQFVMYANYS--NSLTYPNNEDRIAYLTEDVGLXAYYYYFHSHLPFWV 811
E +++ NYS N Y + E ++ Y EDV L AYYYY LP+W+
Sbjct: 198 -----ETYIVNTNYSSKNMREYNDPEYKLDYFMEDVELNAYYYYMREMLPYWM 245
Score = 22.2 bits (45), Expect = 6.7
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +1
Query: 286 NMKAYENFMMMYKVGFLPKNLEFSIFYEKMRE 381
NM+ Y + YK+ + +++E + +Y MRE
Sbjct: 210 NMREYND--PEYKLDYFMEDVELNAYYYYMRE 239
>EF625899-1|ABR45906.1| 1010|Apis mellifera high Glx storage protein
protein.
Length = 1010
Score = 60.5 bits (140), Expect = 2e-11
Identities = 32/120 (26%), Positives = 59/120 (49%), Gaps = 1/120 (0%)
Frame = +1
Query: 130 PEFKTTPVDAAFVEKQKKILSLFYNVNEINYEAEYYKVAQDFNIEASKDCYTNMKAYENF 309
P K D + KQ+ ++ L +++ E + ++IE++ Y N +
Sbjct: 18 PNVKQRAADQDLLNKQQDVIQLLQKISQPIPNQELQNLGASYDIESNSHQYKNPIIVMYY 77
Query: 310 MMMYKVGFL-PKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVL 486
K G + P+ FS ++R+E L+++ AKD++ F KTA +ARV++N+G L
Sbjct: 78 AGAVKAGLVQPQGTTFSNSISQLRKEVSLLYRILLGAKDYQTFLKTAAWARVHVNEGQFL 137
Score = 48.4 bits (110), Expect = 9e-08
Identities = 30/100 (30%), Positives = 52/100 (52%), Gaps = 1/100 (1%)
Frame = +2
Query: 482 FLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYGI 661
FL A+ A++ R DT + + P YE PQ+ + V + + + + G
Sbjct: 136 FLKAFVAAVLTRQDTQSVIFPPVYEILPQHHLDSRVIQEAQNIAIQN---------TQG- 185
Query: 662 XKENEQFVMY-ANYSNSLTYPNNEDRIAYLTEDVGLXAYY 778
K N+Q ++ NYS L++ +E +++Y T+D+GL AYY
Sbjct: 186 -KNNQQNILIPVNYSALLSH--DEQQLSYFTQDIGLAAYY 222
>AY242387-1|AAO72539.2| 693|Apis mellifera prophenoloxidase
protein.
Length = 693
Score = 36.7 bits (81), Expect = 3e-04
Identities = 25/109 (22%), Positives = 48/109 (44%)
Frame = +2
Query: 479 MFLYAYYIAIIQRSDTANFVLPAPYEAYPQYFVXMEVXNKMDYVKMMDGCLDEKICYNYG 658
+F+YA +AI+ R DT + +P E +P K MD + +
Sbjct: 124 LFIYALSVAILHRPDTKDLPVPPLTEVFPD--------------KYMDSGIFSRAREEAN 169
Query: 659 IXKENEQFVMYANYSNSLTYPNNEDRIAYLTEDVGLXAYYYYFHSHLPF 805
+ E + + + + + E R+AY ED+G+ +++++H PF
Sbjct: 170 VVPEGARVPIEIPRDYTASDLDVEHRVAYWREDIGINLHHWHWHLVYPF 218
Score = 27.5 bits (58), Expect = 0.18
Identities = 21/80 (26%), Positives = 35/80 (43%)
Frame = +1
Query: 334 LPKNLEFSIFYEKMREEAIALFKLFYYAKDFECFYKTACYARVYMNQGNVLIRLLHSYYP 513
L + FS+F R+ A L +F + +E F A Y R +N N+ I ++
Sbjct: 76 LGRRQPFSLFIPAHRKIAARLIDIFMGMRTYEDFLSVAVYCRDRLNP-NLFI---YALSV 131
Query: 514 ALRHRQLRSTCSIRSLSSIF 573
A+ HR + L+ +F
Sbjct: 132 AILHRPDTKDLPVPPLTEVF 151
>DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase
isoform B protein.
Length = 931
Score = 25.4 bits (53), Expect = 0.71
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3
Query: 249 GLQHRGQQGLLHKHESLRKFHDDVQGRIPSQEFG 350
G+++ QGL+H+ L+ D++ R +FG
Sbjct: 709 GIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFG 742
>DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase
isoform A protein.
Length = 969
Score = 25.4 bits (53), Expect = 0.71
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3
Query: 249 GLQHRGQQGLLHKHESLRKFHDDVQGRIPSQEFG 350
G+++ QGL+H+ L+ D++ R +FG
Sbjct: 747 GIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFG 780
>DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chloride
channel protein.
Length = 447
Score = 23.8 bits (49), Expect = 2.2
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -2
Query: 824 LTRVPPRTVSVSGNSNNKHXGQHLQLSKQFCLRCW 720
L R P T+SV +H H+Q K+ C R W
Sbjct: 373 LVRQPEDTMSVD---RMQHCELHMQPRKKNCCRSW 404
>DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chloride
channel protein.
Length = 447
Score = 23.8 bits (49), Expect = 2.2
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -2
Query: 824 LTRVPPRTVSVSGNSNNKHXGQHLQLSKQFCLRCW 720
L R P T+SV +H H+Q K+ C R W
Sbjct: 373 LVRQPEDTMSVD---RMQHCELHMQPRKKNCCRSW 404
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 21.8 bits (44), Expect = 8.8
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2
Query: 740 AYLTEDVGLXAYYYYF 787
A L D G+ YYY+F
Sbjct: 464 AQLFADRGMKVYYYFF 479
>AB208108-1|BAE72140.1| 92|Apis mellifera Broad complex zinc
finger domain-Z3 isoform protein.
Length = 92
Score = 21.8 bits (44), Expect = 8.8
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = +3
Query: 234 LQSRPGLQHRGQQGLLHK 287
L + LQHRG G+L +
Sbjct: 51 LTTHKSLQHRGSSGMLKR 68
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 21.8 bits (44), Expect = 8.8
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2
Query: 740 AYLTEDVGLXAYYYYF 787
A L D G+ YYY+F
Sbjct: 464 AQLFADRGMKVYYYFF 479
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 223,316
Number of Sequences: 438
Number of extensions: 4691
Number of successful extensions: 40
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31
length of database: 146,343
effective HSP length: 58
effective length of database: 120,939
effective search space used: 29267238
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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