Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_D19
(856 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 184 7e-47
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 162 2e-40
At4g08140.1 68417.m01344 hypothetical protein weak similarity to... 66 2e-11
At3g14350.3 68416.m01816 leucine-rich repeat transmembrane prote... 31 0.98
At3g14350.2 68416.m01814 leucine-rich repeat transmembrane prote... 31 0.98
At3g14350.1 68416.m01815 leucine-rich repeat transmembrane prote... 31 0.98
At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA) si... 31 0.98
At4g09350.1 68417.m01544 DNAJ heat shock N-terminal domain-conta... 31 1.3
At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD) 30 1.7
At5g16030.1 68418.m01874 expressed protein 30 1.7
At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC) ri... 30 1.7
At3g50370.1 68416.m05508 expressed protein 30 1.7
At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB) si... 30 1.7
At4g26630.1 68417.m03837 expressed protein 30 2.3
At4g08430.1 68417.m01392 Ulp1 protease family protein similar to... 29 3.9
At3g49610.1 68416.m05422 hypothetical protein contains Pfam prof... 29 5.2
At1g48790.1 68414.m05460 mov34 family protein similar to AMSH [H... 28 6.9
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 184 bits (448), Expect = 7e-47
Identities = 94/231 (40%), Positives = 147/231 (63%), Gaps = 5/231 (2%)
Frame = +1
Query: 106 NKMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQEELNMLVDKLLGNEVDLYFPALQML 285
++ T+K + +PKK+ + ++DLSEED L++ L + V+++ +L AL+ +
Sbjct: 17 DEATLKVPSKDPKKKDEKK----DEDLSEEDLELKQNLELYVERVQDPNPELQKAALESM 72
Query: 286 SNLIRTSTTSMTSVPKPLKFLREHYPALKQVYEKITDEKTKKFCADVISVLAMGVSGTLE 465
IR ST+SMTSVPKPLKFLR HY LK +E + D KK+ +D++SVLA+ T+
Sbjct: 73 RQEIRASTSSMTSVPKPLKFLRPHYGTLKAFHETMADSDLKKYLSDILSVLAL----TMS 128
Query: 466 VAEKRECLKYCLLGTLSNVGDWGHEYVRQLEGEIAEEWNIE-----NMDSLLPLVRDVIT 630
+RE L++ L+GT ++G WGHEYVR L GEIA+E+ ++D L+ LV+ ++
Sbjct: 129 ADGERESLRFRLIGTEGDIGSWGHEYVRNLAGEIAQEYTKRQSEEASIDDLMELVQQIVA 188
Query: 631 FDMKHSAXIQACXLLMEIDRLDLLTQHMDQSNYPRVCLYLIGCASXVVEPE 783
F MKH+A +A LLM+++ LDLL +H+D++N+ R C YL A + P+
Sbjct: 189 FHMKHNAETEAVDLLMDVEDLDLLLEHVDKTNFKRTCNYLTSAARYLPGPD 239
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
putative contains Pfam domain PF01851:
Proteasome/cyclosome repeat
Length = 1103
Score = 162 bits (394), Expect = 2e-40
Identities = 94/245 (38%), Positives = 142/245 (57%), Gaps = 27/245 (11%)
Frame = +1
Query: 130 TDEPKKEKAEPAVAPNDDLSEEDKRLQEELNMLVDKLLGNEVDLYFPALQMLSNLIRTST 309
T P K+ + +DLSEED +L++ L + V+++ +L AL+ + IR ST
Sbjct: 21 TKIPSKDSKKKDDKKEEDLSEEDLQLKQNLELYVERVQDPNPELQKIALESMRKEIRDST 80
Query: 310 TSMTSVPKPLKFLREHYPALKQVYEKITDEKTKKFCADVISVLAMGVSGTLE-------V 468
+SMTSVPKPLKFLR HY LK+ + K+ + KK AD++SVLA+ +S E +
Sbjct: 81 SSMTSVPKPLKFLRPHYGVLKEFHAKMAESDLKKMLADILSVLALTMSAEGERICVLWFL 140
Query: 469 AEK---------------RECLKYCLLGTLSNVGDWGHEYVRQLEGEIAEEWNIE----- 588
E +E L Y L G+ S++G WGHEYVR L GEIA+E+ I
Sbjct: 141 VEFDLSYLLLILCYAILFQESLNYRLNGSESDIGSWGHEYVRNLAGEIAKEYTIRQGEES 200
Query: 589 NMDSLLPLVRDVITFDMKHSAXIQACXLLMEIDRLDLLTQHMDQSNYPRVCLYLIGCASX 768
+++ L+ LV+ +++F MKH+A +A LLM+++ LDLL +H+D +N+ R C YL A
Sbjct: 201 SIEDLMDLVQQIVSFHMKHNAETEAVDLLMDVEDLDLLLEHVDNTNFRRTCNYLTSAAKY 260
Query: 769 VVEPE 783
+ P+
Sbjct: 261 LPGPD 265
>At4g08140.1 68417.m01344 hypothetical protein weak similarity to
SP|Q13200 26S proteasome non-ATPase regulatory subunit 2
(26S proteasome regulatory subunit S2) (26S proteasome
subunit p97) (Tumor necrosis factor type 1 receptor
associated protein 2) {Homo sapiens}
Length = 84
Score = 66.5 bits (155), Expect = 2e-11
Identities = 32/63 (50%), Positives = 42/63 (66%)
Frame = +1
Query: 256 DLYFPALQMLSNLIRTSTTSMTSVPKPLKFLREHYPALKQVYEKITDEKTKKFCADVISV 435
DL AL+ + IR T+SMTSVPKPLKFLR Y LK +E + D KK+ +D++SV
Sbjct: 8 DLQKAALESMRQEIRALTSSMTSVPKPLKFLRPQYVTLKAFHETMADSNLKKYISDILSV 67
Query: 436 LAM 444
LA+
Sbjct: 68 LAL 70
>At3g14350.3 68416.m01816 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 689
Score = 31.1 bits (67), Expect = 0.98
Identities = 17/55 (30%), Positives = 30/55 (54%)
Frame = +1
Query: 46 SIHTVGKVILFYLIKRKR*VNKMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQ 210
S+ V VI F+LIKRKR +K + ++ +P + ++D +E+K +Q
Sbjct: 299 SLIVVTAVIAFFLIKRKR--SKRSSSTDIEKTDNNINQPIILASNDFHQENKSVQ 351
>At3g14350.2 68416.m01814 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 680
Score = 31.1 bits (67), Expect = 0.98
Identities = 17/55 (30%), Positives = 30/55 (54%)
Frame = +1
Query: 46 SIHTVGKVILFYLIKRKR*VNKMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQ 210
S+ V VI F+LIKRKR +K + ++ +P + ++D +E+K +Q
Sbjct: 262 SLIVVTAVIAFFLIKRKR--SKRSSSTDIEKTDNNINQPIILASNDFHQENKSVQ 314
>At3g14350.1 68416.m01815 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 1 GB:AAC27894 from [Zea
mays]
Length = 717
Score = 31.1 bits (67), Expect = 0.98
Identities = 17/55 (30%), Positives = 30/55 (54%)
Frame = +1
Query: 46 SIHTVGKVILFYLIKRKR*VNKMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQ 210
S+ V VI F+LIKRKR +K + ++ +P + ++D +E+K +Q
Sbjct: 299 SLIVVTAVIAFFLIKRKR--SKRSSSTDIEKTDNNINQPIILASNDFHQENKSVQ 351
>At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA)
similar to ribosomal protein L13A GB:O49885 [Lupinus
luteus]
Length = 206
Score = 31.1 bits (67), Expect = 0.98
Identities = 17/45 (37%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +1
Query: 442 MGVSGTLEVAEKRECLKYCLLGTLSNVGDWGH-EYVRQLEGEIAE 573
M V L+V + KYCLLG LS+ W H + +++LE + E
Sbjct: 123 MVVPDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELENKRKE 167
>At4g09350.1 68417.m01544 DNAJ heat shock N-terminal
domain-containing protein similar to SP|Q45552 Chaperone
protein dnaJ {Bacillus stearothermophilus}; contains
Pfam profile PF00226: DnaJ domain
Length = 249
Score = 30.7 bits (66), Expect = 1.3
Identities = 17/48 (35%), Positives = 27/48 (56%)
Frame = +1
Query: 271 ALQMLSNLIRTSTTSMTSVPKPLKFLREHYPALKQVYEKITDEKTKKF 414
A + LS TTS+ PLK E + L++VY ++DE+T++F
Sbjct: 125 AYRRLSKEYHPDTTSL-----PLKTASEKFMKLREVYNVLSDEETRRF 167
>At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD)
Length = 206
Score = 30.3 bits (65), Expect = 1.7
Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +1
Query: 442 MGVSGTLEVAEKRECLKYCLLGTLSNVGDWGH-EYVRQLEGEIAEEWNI 585
M + L+V + KYCLLG LS+ W H + +++LE + E ++
Sbjct: 123 MVIPDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELETKRKERAHV 171
>At5g16030.1 68418.m01874 expressed protein
Length = 339
Score = 30.3 bits (65), Expect = 1.7
Identities = 13/45 (28%), Positives = 28/45 (62%)
Frame = +1
Query: 82 LIKRKR*VNKMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQEE 216
L ++++ K + N+ DE ++E+ + ++ DD EED++ +EE
Sbjct: 255 LEEKEKEEEKQDMSNEEDEEEEEEEKQDMSEEDDKEEEDEQEEEE 299
>At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC)
ribosomal protein L13a -Lupinus luteus,PID:e1237871
Length = 206
Score = 30.3 bits (65), Expect = 1.7
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 442 MGVSGTLEVAEKRECLKYCLLGTLSNVGDWGH-EYVRQLE 558
M + L+V + KYCLLG LS+ W H + +++LE
Sbjct: 123 MVIPDALKVLRLQSGHKYCLLGRLSSEVGWNHYDTIKELE 162
>At3g50370.1 68416.m05508 expressed protein
Length = 2179
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = -3
Query: 578 HSSAISPSNCLTYSCPQSPTFDNVPRRQYFKHSLFSATSRVPLTPIAKTEITSAQ 414
HS+A+ PS+ T P S T + F+ LFS S +P +P +I S Q
Sbjct: 1108 HSAALHPSSLQTAIPPVSTTSAQMEEPVKFQFGLFSGPSLIP-SPFPAIQIGSIQ 1161
>At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB)
similar to 60S RIBOSOMAL PROTEIN L13A GB:P35427 from
[Rattus norvegicus]
Length = 206
Score = 30.3 bits (65), Expect = 1.7
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +1
Query: 433 VLAMGVSGTLEVAEKRECLKYCLLGTLSNVGDWGH-EYVRQLE 558
V M + L+V + KYCLLG LS+ W H + +++LE
Sbjct: 120 VKRMVIPDALKVLRLQAGHKYCLLGRLSSEVGWNHYDTIKELE 162
>At4g26630.1 68417.m03837 expressed protein
Length = 763
Score = 29.9 bits (64), Expect = 2.3
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +1
Query: 109 KMTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQEELNMLVDK 237
K+ KNKT+E KK+ AEP P D +++ E L L+DK
Sbjct: 295 KVREKNKTEEVKKD-AEPR-TPFSDRPVRERKSVERLVALIDK 335
>At4g08430.1 68417.m01392 Ulp1 protease family protein similar to
At5g45570, At5g28235; contains Pfam profile PF02902:
Ulp1 protease family, C-terminal catalytic domain
Length = 808
Score = 29.1 bits (62), Expect = 3.9
Identities = 31/125 (24%), Positives = 54/125 (43%), Gaps = 10/125 (8%)
Frame = +1
Query: 88 KRKR*VNKMTVK-NKTDEPKKEKAEPAVAPNDDLSEED-KRLQEELNMLVDKLLGNEVDL 261
K K + K VK K D PK EK + VAP ++D + +++N K+ +
Sbjct: 392 KPKPEMKKTAVKVEKVDSPKPEKKKSVVAPTTKTFDDDVVDVTDKVNADNLKMASRSEET 451
Query: 262 YFPALQMLSNLIRTSTTSMTSVPKPLKFL------REHYPAL--KQVYEKITDEKTKKFC 417
+ L ++N + +T ++ ++ + L L R P L Q Y + + K+
Sbjct: 452 FSNPLDQMAN--KATTDALRALQEGLDNLDGITSKRRRVPQLAGSQKYPSVGNSTVKRII 509
Query: 418 ADVIS 432
DV S
Sbjct: 510 TDVPS 514
>At3g49610.1 68416.m05422 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 334
Score = 28.7 bits (61), Expect = 5.2
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 6/58 (10%)
Frame = -3
Query: 647 ECFMSNVITSRTSGNSESMFSIFHSSAI------SPSNCLTYSCPQSPTFDNVPRRQY 492
ECF + V T R N + S+F++ SP+ + S P S +N R++Y
Sbjct: 108 ECFFTRVYTRRNHNNVSTSSSLFNTDEFERTETKSPTIRNSQSSPSSCLMENTKRKRY 165
>At1g48790.1 68414.m05460 mov34 family protein similar to AMSH [Homo
sapiens] GI:4098124; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 507
Score = 28.3 bits (60), Expect = 6.9
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +1
Query: 112 MTVKNKTDEPKKEKAEPAVAPNDDLSEEDKRLQEELNMLV 231
M DEP+K EP+VA N+ + K EEL+ ++
Sbjct: 236 MITNGSNDEPEKPIVEPSVASNEKI---QKNYTEELSSMI 272
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,548,310
Number of Sequences: 28952
Number of extensions: 323146
Number of successful extensions: 1111
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1057
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1107
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1989897600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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