Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_N01
(837 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 100 2e-21
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 97 1e-20
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 44 2e-04
At4g02520.1 68417.m00345 glutathione S-transferase, putative 42 5e-04
At2g02930.1 68415.m00241 glutathione S-transferase, putative 41 9e-04
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 39 0.004
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 38 0.006
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 38 0.006
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 35 0.058
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 34 0.10
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 33 0.18
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 33 0.18
At1g16840.2 68414.m02028 expressed protein 32 0.54
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 31 1.3
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 30 1.7
At5g46550.1 68418.m05731 DNA-binding bromodomain-containing prot... 30 2.2
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 30 2.2
At5g22030.2 68418.m02564 ubiquitin-specific protease 8, putative... 29 3.8
At5g22030.1 68418.m02563 ubiquitin-specific protease 8, putative... 29 3.8
At3g49210.1 68416.m05378 expressed protein 29 3.8
At4g22130.1 68417.m03199 protein kinase family protein contains ... 29 5.1
At5g53060.1 68418.m06592 KH domain-containing protein 28 6.7
At2g46820.1 68415.m05842 expressed protein 28 6.7
At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Ex... 28 8.8
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 99.5 bits (237), Expect = 2e-21
Identities = 59/143 (41%), Positives = 78/143 (54%), Gaps = 4/143 (2%)
Frame = +2
Query: 92 NFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESN 271
N A KALIAA+Y G + V +F G TNK+ FLK P GKVP E+ +G V ESN
Sbjct: 11 NKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGSVF--ESN 68
Query: 272 AIAYYVA----NESLRGGDLATQARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVE 439
AIA YV+ + SL G L A++ QW +S E+ + W P +G M ++ E
Sbjct: 69 AIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFMPYSAPAEE 128
Query: 440 RAKSDLLAALXVLDGHLLTRTFL 508
A S L AL L+ HL + T+L
Sbjct: 129 GAISTLKRALDALNTHLTSNTYL 151
Score = 30.3 bits (65), Expect = 1.7
Identities = 18/53 (33%), Positives = 25/53 (47%)
Frame = +3
Query: 507 LVTERIXLADVIVFSTLLHAFQHVLXPSVRSSLINVXRWFLTVAHHPPVSXVV 665
LV I LAD+I L F V+ S +V R+F TV + P + V+
Sbjct: 151 LVGHSITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVL 203
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 97.1 bits (231), Expect = 1e-20
Identities = 58/143 (40%), Positives = 77/143 (53%), Gaps = 4/143 (2%)
Frame = +2
Query: 92 NFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESN 271
N A KALIAA+Y+G ++ + +F G TNKS +FLK P GKVP E+ +G + ESN
Sbjct: 11 NKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGPIF--ESN 68
Query: 272 AIAYYVA----NESLRGGDLATQARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVE 439
AIA YV+ + SL G L A + QW +S E+ W P +G F+ E
Sbjct: 69 AIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYAPFSAPAEE 128
Query: 440 RAKSDLLAALXVLDGHLLTRTFL 508
A S L L L+ HL + TFL
Sbjct: 129 AAISALKRGLEALNTHLASNTFL 151
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/53 (28%), Positives = 25/53 (47%)
Frame = +3
Query: 507 LVTERIXLADVIVFSTLLHAFQHVLXPSVRSSLINVXRWFLTVAHHPPVSXVV 665
LV + LAD++ L F V+ S+ +V R+F T+ + P V+
Sbjct: 151 LVGHSVTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVL 203
>At1g02930.1 68414.m00260 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 208
Score = 43.6 bits (98), Expect = 2e-04
Identities = 28/84 (33%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
Frame = +2
Query: 86 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 265
P + + LIA D + + +K E F+ + P GKVPAFE D K+ E
Sbjct: 10 PASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIF--E 67
Query: 266 SNAIAYYVANE-SLRGGDLATQAR 334
S AI Y+A+E S +G +L + +
Sbjct: 68 SRAITQYIAHEFSDKGNNLLSTGK 91
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 41.9 bits (94), Expect = 5e-04
Identities = 25/70 (35%), Positives = 36/70 (51%)
Frame = +2
Query: 86 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 265
P + + LIA D ++ + +K E FL + P G+VPAFE DG + L E
Sbjct: 10 PASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE--DGDLKLFE 67
Query: 266 SNAIAYYVAN 295
S AI Y+A+
Sbjct: 68 SRAITQYIAH 77
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 41.1 bits (92), Expect = 9e-04
Identities = 25/70 (35%), Positives = 36/70 (51%)
Frame = +2
Query: 86 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 265
P + + LIA D ++ + +K E FL + P G+VPAFE DG + L E
Sbjct: 10 PASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE--DGDLKLFE 67
Query: 266 SNAIAYYVAN 295
S AI Y+A+
Sbjct: 68 SRAITQYIAH 77
>At1g02920.1 68414.m00259 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]; supported by cDNA GI:443697.
Length = 209
Score = 39.1 bits (87), Expect = 0.004
Identities = 25/70 (35%), Positives = 34/70 (48%)
Frame = +2
Query: 86 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 265
P + + LIA D + + +K E F+ + P GKVPAFE DG L E
Sbjct: 10 PASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFE--DGDFKLFE 67
Query: 266 SNAIAYYVAN 295
S AI Y+A+
Sbjct: 68 SRAITQYIAH 77
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 38.3 bits (85), Expect = 0.006
Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = +2
Query: 179 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 319
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 38.3 bits (85), Expect = 0.006
Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = +2
Query: 179 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 319
+K+E FL P G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107
>At2g02380.1 68415.m00176 glutathione S-transferase, putative
similar to gi:167970 gb:AAA72320 gb:AY052332
Length = 223
Score = 35.1 bits (77), Expect = 0.058
Identities = 24/64 (37%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 101 AYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAI 277
A++ IA G D + P N + G+ + S DF K P G VPA DG V++ +S AI
Sbjct: 23 AHRVRIALTLKGLDYEYIPVNLLKGDQSDS-DFKKINPMGTVPAL--VDGDVVINDSFAI 79
Query: 278 AYYV 289
Y+
Sbjct: 80 IMYL 83
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 34.3 bits (75), Expect = 0.10
Identities = 20/48 (41%), Positives = 27/48 (56%)
Frame = +2
Query: 149 VAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA 292
+ N + G+ K FL P G+VP F DG + LTES AI+ Y+A
Sbjct: 67 ITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGGLKLTESRAISEYIA 111
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 33.5 bits (73), Expect = 0.18
Identities = 24/64 (37%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = +2
Query: 101 AYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAI 277
A++ IA G D + P N + G+ S DF K P G VPA DG V++ +S AI
Sbjct: 20 AHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGDVVINDSFAI 76
Query: 278 AYYV 289
Y+
Sbjct: 77 IMYL 80
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 33.5 bits (73), Expect = 0.18
Identities = 24/64 (37%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = +2
Query: 101 AYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAI 277
A++ IA G D + P N + G+ S DF K P G VPA DG V++ +S AI
Sbjct: 20 AHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGDVVINDSFAI 76
Query: 278 AYYV 289
Y+
Sbjct: 77 IMYL 80
>At1g16840.2 68414.m02028 expressed protein
Length = 161
Score = 31.9 bits (69), Expect = 0.54
Identities = 20/61 (32%), Positives = 26/61 (42%)
Frame = +3
Query: 300 VSAEEIWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNSTNRMLNVQSLTYWPPXKYW 479
V W+P P SGS HG+ L+ L + LT STN S Y+ YW
Sbjct: 83 VPGASYWVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLSTNSSHGWPSSDYFLKGSYW 139
Query: 480 T 482
+
Sbjct: 140 S 140
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 30.7 bits (66), Expect = 1.3
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = +3
Query: 405 LVSCNSTNRMLNVQSLTYWPPXKYWTDIFSHAPSLVTE 518
L++ + N N +S + WPP K W D H + E
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSRE 232
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 30.3 bits (65), Expect = 1.7
Identities = 24/70 (34%), Positives = 34/70 (48%), Gaps = 7/70 (10%)
Frame = +2
Query: 101 AYKALIAAQYSGTDVKVAP-NFVFGETNKS------EDFLKKFPAGKVPAFESADGKVLL 259
A++ IA G D + P N + G+ S +DF K P G VPA DG V++
Sbjct: 20 AHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL--VDGDVVI 77
Query: 260 TESNAIAYYV 289
+S AI Y+
Sbjct: 78 NDSFAIIMYL 87
>At5g46550.1 68418.m05731 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 494
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2
Query: 83 IPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSE 190
+P A++ AQY+GT +K V G+ NK++
Sbjct: 333 VPPEKALRAAILKAQYAGTIIKAKHRIVLGQNNKAD 368
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 29.9 bits (64), Expect = 2.2
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = -3
Query: 610 FIXDERTLGXSTCWKACSSVLKTMTSASXILSVTKEGA 497
F+ ++ +C K CSS LKT+ S + S KE A
Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEA 193
>At5g22030.2 68418.m02564 ubiquitin-specific protease 8, putative
(UBP8) similar to ubiquitin-specific protease 8 partial
sequence GI:11993469 [Arabidopsis thaliana]
Length = 622
Score = 29.1 bits (62), Expect = 3.8
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2
Query: 59 WRPGYFTLIPENFRAYKALIAAQYSG 136
W PG T+ P F+A A A Q+SG
Sbjct: 90 WAPGASTVAPRTFKAKLARFAPQFSG 115
>At5g22030.1 68418.m02563 ubiquitin-specific protease 8, putative
(UBP8) similar to ubiquitin-specific protease 8 partial
sequence GI:11993469 [Arabidopsis thaliana]
Length = 622
Score = 29.1 bits (62), Expect = 3.8
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2
Query: 59 WRPGYFTLIPENFRAYKALIAAQYSG 136
W PG T+ P F+A A A Q+SG
Sbjct: 90 WAPGASTVAPRTFKAKLARFAPQFSG 115
>At3g49210.1 68416.m05378 expressed protein
Length = 518
Score = 29.1 bits (62), Expect = 3.8
Identities = 16/64 (25%), Positives = 30/64 (46%)
Frame = +2
Query: 314 DLATQARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALXVLDGHLL 493
D+ + +W +W + P S L ++ K+ ++R K+ L AAL + G +
Sbjct: 352 DMMAKGSTCRWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFI 411
Query: 494 TRTF 505
+TF
Sbjct: 412 LKTF 415
>At4g22130.1 68417.m03199 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 338
Score = 28.7 bits (61), Expect = 5.1
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%)
Frame = +2
Query: 98 RAYKALIAAQYSGTDVKVAPNF-----VFGETNKSEDFLKKFPAGKVPAFESADGKVL-L 259
R + A+QY+ + ++VA N + GE + + +FP GK+ A + D L L
Sbjct: 8 RIRSPITASQYTVSSLQVATNSFSQENIIGEGSLGRVYRAEFPNGKIMAIKKIDNAALSL 67
Query: 260 TESNAIAYYVANES 301
E + V+N S
Sbjct: 68 QEEDNFLEAVSNMS 81
>At5g53060.1 68418.m06592 KH domain-containing protein
Length = 652
Score = 28.3 bits (60), Expect = 6.7
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 442 CKV*PTGRLXSTGRTSSHTHLPWLPRESHLPMSLS 546
C + G++ R + TH+ LPRES+LP +S
Sbjct: 189 CLLGKGGKIIEQMRIETKTHIRILPRESNLPRCVS 223
>At2g46820.1 68415.m05842 expressed protein
Length = 174
Score = 28.3 bits (60), Expect = 6.7
Identities = 22/65 (33%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = +1
Query: 454 PTGRLXSTGRTS-SHTHLPWLPRESHLPMSLSSVHCCMLSSTCXTRASVRX**TFXVGS* 630
P RL S +S S LP LP +SH + ++ +C + TRA T VG
Sbjct: 18 PPSRLASASASSPSCISLPTLPIQSHTRAAKATAYCRKIVRNVVTRA------TTEVGEA 71
Query: 631 PSPTT 645
P+ TT
Sbjct: 72 PATTT 76
>At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to
Exportin1 (XPO1) protein [Arabidopsis thaliana]
GI:7671510
Length = 1075
Score = 27.9 bits (59), Expect = 8.8
Identities = 20/81 (24%), Positives = 37/81 (45%), Gaps = 2/81 (2%)
Frame = -3
Query: 550 LKTMTSASXILSVTKEGACEKMSVQYX*GGQ*VRLCT--FNILFVELHDTKVREDPSAGS 377
++ + S ++S+ G +++ Y + ++C +N L +EL D D A S
Sbjct: 325 IRVLESTPEVVSLLLAGLEYLINISYVDDTEVFKVCLDYWNSLVLELFDAHHNSDNPAVS 384
Query: 376 RQ*FAVRP*CPLPDTGLGSQI 314
++P P GLGSQ+
Sbjct: 385 ASLMGLQPFLPGMVDGLGSQV 405
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,779,978
Number of Sequences: 28952
Number of extensions: 392623
Number of successful extensions: 1006
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1931371200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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