Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0006_G11
(578 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa s... 161 3e-40
At5g22330.1 68418.m02605 TATA box-binding protein-interacting pr... 32 0.32
At1g01450.1 68414.m00060 protein kinase-related contains eukaryo... 29 3.0
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 28 5.2
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat... 28 5.2
At5g38070.1 68418.m04587 zinc finger (C3HC4-type RING finger) fa... 27 6.8
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 27 6.8
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 27 6.8
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain... 27 9.0
At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) / isoch... 27 9.0
At1g48650.1 68414.m05445 helicase domain-containing protein cont... 27 9.0
>At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa
subunit, putative similar to NADH-ubiquinone
oxidoreductase 24 kDa subunit, mitochondrial precursor
(EC 1.6.5.3) (EC 1.6.99.3) (Polypeptide II)
(Swiss-Prot:P04394) [Bos taurus]
Length = 255
Score = 161 bits (391), Expect = 3e-40
Identities = 71/143 (49%), Positives = 103/143 (72%)
Frame = +1
Query: 148 SLFVHRDTPEDNPSIPFEFTAPNQKRVEALLAIYPEGHKRGAMIPLLDLAQRXNGGWLPT 327
+L H D+P++ P +P+EF+ NQ +V+ +L+ YP +K+ A+IPLLDLAQ+ NGGWLP
Sbjct: 31 ALNYHLDSPDNKPDLPWEFSEANQSKVKEILSYYPSNYKQSAVIPLLDLAQQQNGGWLPV 90
Query: 328 SAMHKVAEILNLPRMRVYEVATFYTMFIRRPIGKYHVQVCTTTPCWLRGSDAVLKAIKEA 507
SAM+ VA+++ + +RVYEVATFY+MF R +GKYH+ VC TTPC +RGS + A+ +
Sbjct: 91 SAMNAVAKVIEVAPIRVYEVATFYSMFNRAKVGKYHLLVCGTTPCMIRGSRDIESALLDH 150
Query: 508 TGCEVGGNSPCGKFSVSEVECLG 576
G + G + G FSV E+EC+G
Sbjct: 151 LGVKRGEVTKDGLFSVGEMECMG 173
>At5g22330.1 68418.m02605 TATA box-binding protein-interacting
protein-related similar to TATA box-binding
protein-interacting protein SP:O35753 from [ Mus
musculus]
Length = 458
Score = 31.9 bits (69), Expect = 0.32
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = -3
Query: 168 VSVDEETVMLEGRSGLQTSRRHAPQGLNPS 79
++VDEE ++L G G +TS RHA Q L+P+
Sbjct: 386 LTVDEECLVLLGEIGQRTSLRHAVQLLSPA 415
>At1g01450.1 68414.m00060 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 470
Score = 28.7 bits (61), Expect = 3.0
Identities = 17/62 (27%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = +2
Query: 56 K*CYLASKLGFRPCGACLLEVCRPLLPSSMTVSSSTETPLKTTL-VSRLNLQRLIKRELK 232
K C+L +L + G + EVC P +++++ + + L+ L + L+ +R+ EL
Sbjct: 92 KECFLVMELMRKTLGMHIKEVCGPRKKNTLSLPVAVDLMLQIALGMEYLHSKRIYHGELN 151
Query: 233 PS 238
PS
Sbjct: 152 PS 153
>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related low similarity to clathrin assembly
protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
profile PF01417: ENTH domain
Length = 591
Score = 27.9 bits (59), Expect = 5.2
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +1
Query: 238 LAIYPEGHKRGAMIPLLDLAQRXNGGW 318
LAIYP GH+ A L L + GW
Sbjct: 404 LAIYPPGHETSAPSNSLSLIEAGGSGW 430
>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA
polymerase II subunit A) {Homo sapiens}; contains Pfam
profiles: PF03175 DNA polymerase type B, organellar and
viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2271
Score = 27.9 bits (59), Expect = 5.2
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = -3
Query: 468 KPAWSRCTHLYMILSYWSPNKHSIEC 391
+P WS HL + W N HS +C
Sbjct: 1922 EPKWSVARHLPEYIEVWRQNPHSNQC 1947
>At5g38070.1 68418.m04587 zinc finger (C3HC4-type RING finger)
family protein contains InterPro Entry IPR001841
Zn-finger, RING; contains PROSITE PS00190: Cytochrome c
family heme-binding site signature
Length = 259
Score = 27.5 bits (58), Expect = 6.8
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -3
Query: 222 LLIRRCKFKRDTRVVFRGVSVDEET 148
++I+RC+ +RDTR+ G S DEET
Sbjct: 217 IVIQRCRQERDTRL--SGFSSDEET 239
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/48 (29%), Positives = 26/48 (54%)
Frame = +1
Query: 241 AIYPEGHKRGAMIPLLDLAQRXNGGWLPTSAMHKVAEILNLPRMRVYE 384
AI+ + ++G I ++ A R G +PT+ HK +I + P ++ E
Sbjct: 76 AIFKDPFRKGNNILVMCDAYRPAGDPIPTNNRHKAVKIFDHPNVKAEE 123
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 27.5 bits (58), Expect = 6.8
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = +1
Query: 307 NGGWLPTSAMHKVAEILNLPRMRVYEVATFYTMFIRRPIGKYHVQVCTTTPCWLRGSD 480
N +L +++ +A++ +LP ++ MF+ RP + V T P W R D
Sbjct: 190 NSSFLASNSF-TMADLYHLPNIQYLMDTHTKRMFVNRPSVRRWVAEITARPAWKRACD 246
>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Chain B, Crystal Structure Of N-Terminal Domain Of
Drosophila Ap180 (GP:13399617) [Drosophila
melanogaster]; supporting cDNA
gi|20465326|gb|AY096427.1|
Length = 601
Score = 27.1 bits (57), Expect = 9.0
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = +1
Query: 238 LAIYPEGHKRGAMIPLLDLAQRXNGGW 318
LAIYP GH+ +L L + GW
Sbjct: 415 LAIYPPGHEAPGPSNILSLIETGGSGW 441
>At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) /
isochorismate mutase identical to GI:17223087 and
GB:AF078080; contains Pfam profile PF00425: chorismate
binding enzyme; contains TIGRfam profile TIGR00543:
isochorismate synthases; identical to cDNA isochorismate
synthase 1 precursor (ICS1) nuclear gene for plastid
product GI:17223086
Length = 622
Score = 27.1 bits (57), Expect = 9.0
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -3
Query: 435 MILSYWSPNKHSIECCNFIHSHS 367
M+L Y +PN +S+ CC F S S
Sbjct: 565 MVLCYQNPNFYSLFCCCFCSSPS 587
>At1g48650.1 68414.m05445 helicase domain-containing protein
contains similarity to DEIH-box RNA/DNA helicase
[Arabidopsis thaliana] GI:5881579; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, PF00035:
Double-stranded RNA binding motif
Length = 1197
Score = 27.1 bits (57), Expect = 9.0
Identities = 20/75 (26%), Positives = 32/75 (42%)
Frame = +1
Query: 322 PTSAMHKVAEILNLPRMRVYEVATFYTMFIRRPIGKYHVQVCTTTPCWLRGSDAVLKAIK 501
P + M + R R ++ + ++ P G+ V T P + + DA+LKAI
Sbjct: 255 PETVMQNSLARERILRPRSLQLKSKQQQWVDSPEGQKMVGFRKTLPAY-KEKDALLKAIA 313
Query: 502 EATGCEVGGNSPCGK 546
V G + CGK
Sbjct: 314 ANQVVVVSGETGCGK 328
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,688,990
Number of Sequences: 28952
Number of extensions: 254494
Number of successful extensions: 717
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 695
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1131744440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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