Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_L24
(383 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g30560.1 68417.m04337 cyclic nucleotide-regulated ion channel... 29 1.4
At1g67623.1 68414.m07708 F-box family protein hypothetical prote... 29 1.4
At5g50870.1 68418.m06304 ubiquitin-conjugating enzyme, putative ... 28 2.5
At3g63200.1 68416.m07100 patatin-related low similarity to patat... 28 2.5
At3g03540.1 68416.m00355 phosphoesterase family protein similar ... 27 3.3
At5g49630.1 68418.m06141 amino acid permease 6 (AAP6) identical ... 27 5.7
At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit... 27 5.7
At3g54660.1 68416.m06048 gluthatione reductase, chloroplast near... 27 5.7
At2g41020.1 68415.m05067 WW domain-containing protein contains P... 27 5.7
At1g74950.1 68414.m08697 expressed protein 27 5.7
At4g34140.1 68417.m04845 D111/G-patch domain-containing protein ... 26 9.9
At1g63700.1 68414.m07209 protein kinase, putative contains prote... 26 9.9
>At4g30560.1 68417.m04337 cyclic nucleotide-regulated ion channel,
putative similar to cyclic nucleotide and
calmodulin-regulated ion channel cngc6 GI:4581207 from
[Arabidopsis thaliana]
Length = 733
Score = 28.7 bits (61), Expect = 1.4
Identities = 16/68 (23%), Positives = 30/68 (44%)
Frame = -1
Query: 323 CHSLDLILYQNYLFVDRCVLGGAYVFINVTDVFFSDDCEVEVSIRPSCDAAIKVSHISVR 144
C + L ++N+LF + G + + D +C V + P D I + +S
Sbjct: 312 CSNSSLDCHRNFLFCGNEKMDGYAAWTTIKDSVLQLNCPVNTTDNPPFDFGIYLRALSSG 371
Query: 143 ISSGQTFL 120
I S ++F+
Sbjct: 372 IVSSKSFV 379
>At1g67623.1 68414.m07708 F-box family protein hypothetical protein
; similar to SKP1 interacting partner 2 (SKIP2)
TIGR_Ath1:At5g67250
Length = 296
Score = 28.7 bits (61), Expect = 1.4
Identities = 23/73 (31%), Positives = 33/73 (45%), Gaps = 7/73 (9%)
Frame = -1
Query: 260 GAYVFINVTDVFFSDDCEVEVSIRPSCDAAIKVSHISVRISSGQTFLPS-------NQGF 102
G Y+F + TD+ FS CE+ + I + K S I I F P+ N+G
Sbjct: 220 GWYLFGDETDLDFSSACELYLYINRNFPFEAKKSKIDGEIYWACEFEPNRFVRTEPNRGL 279
Query: 101 RVSVRLHTVQLER 63
SVRL ++ R
Sbjct: 280 DKSVRLRLIEYNR 292
>At5g50870.1 68418.m06304 ubiquitin-conjugating enzyme, putative
strong similarity to ubiquitin conjugating enzyme
[Lycopersicon esculentum] GI:886679; contains Pfam
profile PF00179: Ubiquitin-conjugating enzyme
Length = 192
Score = 27.9 bits (59), Expect = 2.5
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +3
Query: 45 TPIEGRPFQLDCVQPNAYP 101
TP EG FQ+D P+ YP
Sbjct: 45 TPYEGGTFQIDITMPDGYP 63
>At3g63200.1 68416.m07100 patatin-related low similarity to
patatin-like latex protein allergen Hev b 7 - Hevea
brasiliensis, EMBL:AF113546; contains patatin domain
PF01734
Length = 384
Score = 27.9 bits (59), Expect = 2.5
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
Frame = +3
Query: 126 RLSGADPNADVTDFDRRITAGPDGNLYFTIVTKEDVSDIYKYVCTAKNA---AVDEEVVL 296
RL DP+A ++DF + G + ++ +D S + + TA++A ++ L
Sbjct: 57 RLQTGDPHAHISDFFDIVAGTGIGGILAALLVADDGSG--RPMFTARDAVKFVAEKNSEL 114
Query: 297 VEYEIKGVTKDNSGYKGE 350
E GV + N Y G+
Sbjct: 115 FEIRYTGVFRRNKRYSGK 132
>At3g03540.1 68416.m00355 phosphoesterase family protein similar to
SP|P95246 Phospholipase C 2 precursor (EC 3.1.4.3)
{Mycobacterium tuberculosis}; contains Pfam profile
PF04185: Phosphoesterase family
Length = 521
Score = 27.5 bits (58), Expect = 3.3
Identities = 30/94 (31%), Positives = 41/94 (43%), Gaps = 8/94 (8%)
Frame = +3
Query: 27 AKTHEKTPIEGRPFQLDCVQPNAYPKPLITWKKRLSGADPNADVTDFDRR---ITAGPDG 197
A T E P Q DC P P+ K R + A NA+++DF + AG G
Sbjct: 390 AGTFESVITRNSPRQ-DC--PETLSNPV---KMRGTVAKENAELSDFQEELVIVAAGLKG 443
Query: 198 N-----LYFTIVTKEDVSDIYKYVCTAKNAAVDE 284
+ L + + K VSD KYV A + V+E
Sbjct: 444 DYKNEELLYKLCKKTCVSDASKYVTKAFDKFVEE 477
>At5g49630.1 68418.m06141 amino acid permease 6 (AAP6) identical to
amino acid permease 6 (AAP6) [Arabidopsis thaliana]
GI:1769887
Length = 481
Score = 26.6 bits (56), Expect = 5.7
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = -1
Query: 128 TFLPSNQGFRVSVRLHTVQLERPAFDWRFLVCLRWRCDEVSL 3
+F P F + + + ++ + +F W +L L W C VSL
Sbjct: 417 SFWPLTVYFPIEMHIAQKKIPKFSFTWTWLKILSWTCFIVSL 458
>At5g42190.1 68418.m05135 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At2) / UFO-binding protein (UIP2) E3
ubiquitin ligase; skp1b; identical to UIP2 GI:3719211
from [Arabidopsis thaliana]; contains Pfam profiles
PF01466: Skp1 family, dimerisation domain and
PF03931:Skp1 family, tetramerisation domain; identical
to cDNA UFO binding protein UIP2 mRNA, partial cds
GI:3719210
Length = 171
Score = 26.6 bits (56), Expect = 5.7
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 264 KNAAVDEEVVLVEYEIKGVTKDNSGYKGEPVPQYVSK 374
+N +DE V L IK + +D+ G P+P SK
Sbjct: 15 ENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSK 51
>At3g54660.1 68416.m06048 gluthatione reductase, chloroplast nearly
identical to SP|P42770 Glutathione reductase,
chloroplast precursor (EC 1.8.1.7) (GR) (GRASE)
{Arabidopsis thaliana}; identical to cDNA glutathione
reductase GI:451197
Length = 565
Score = 26.6 bits (56), Expect = 5.7
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -1
Query: 269 VLGGAYVFINVTDVFFSDDCEVEVSIR 189
++GG Y+ + +F +CEV V IR
Sbjct: 265 IVGGGYIALEFAGIFNGLNCEVHVFIR 291
>At2g41020.1 68415.m05067 WW domain-containing protein contains Pfam
domain PF00397: WW domain
Length = 463
Score = 26.6 bits (56), Expect = 5.7
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 199 FPSGPAVMRRSKSVTSALGSAPDKRFFQVIK 107
+PS AV+RR+ V S+ P+ +F ++ K
Sbjct: 421 YPSPGAVLRRNAEVASSQKKKPNSQFTEITK 451
>At1g74950.1 68414.m08697 expressed protein
Length = 249
Score = 26.6 bits (56), Expect = 5.7
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 15 IASPAKTHEKTPIEGRPFQLDCVQPNA 95
+ SPAKT + PI+ P L C P A
Sbjct: 180 VCSPAKTAAQEPIQPNPASLACELPIA 206
>At4g34140.1 68417.m04845 D111/G-patch domain-containing protein
contains Pfam PF01585: G-patch domain
Length = 418
Score = 25.8 bits (54), Expect = 9.9
Identities = 14/49 (28%), Positives = 21/49 (42%)
Frame = -1
Query: 188 PSCDAAIKVSHISVRISSGQTFLPSNQGFRVSVRLHTVQLERPAFDWRF 42
P C+A + H+ VR+ +GQ + N + L P DW F
Sbjct: 226 PICEARL---HL-VRVRTGQVYKLRNPSAKYLASLSVYDASNPTKDWEF 270
>At1g63700.1 68414.m07209 protein kinase, putative contains protein
kinase domain, Pfam:PF00069; similar to MEK kinase
(MAP3Ka) [Arabidopsis thaliana] gi|4204912|gb|AAD10848
Length = 883
Score = 25.8 bits (54), Expect = 9.9
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = -1
Query: 221 SDDCEVEVSIRPSCDAAIKVSHISVRISSGQTFLPSNQG 105
S D + S RP DA +K S + + G T +P N G
Sbjct: 96 STDSGMNGSQRPGLDANLKPSWLPLPKPHGATSIPDNTG 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,818,463
Number of Sequences: 28952
Number of extensions: 145068
Number of successful extensions: 460
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 460
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 537681456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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