Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_L13
(691 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 159 2e-39
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 158 3e-39
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 136 1e-32
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 133 1e-31
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 71 6e-13
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 66 2e-11
At2g17150.1 68415.m01980 RWP-RK domain-containing protein simila... 26 0.41
At4g38190.1 68417.m05391 cellulose synthase family protein simil... 31 0.72
At3g21330.1 68416.m02694 basic helix-loop-helix (bHLH) family pr... 29 2.9
At1g30240.1 68414.m03699 PELP1-related contains weak similarity ... 29 2.9
At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family pr... 29 2.9
At5g38396.1 68418.m04641 F-box family protein contains F-box dom... 28 5.1
At1g78810.2 68414.m09186 expressed protein 28 6.7
At1g78810.1 68414.m09185 expressed protein 28 6.7
At1g12130.1 68414.m01405 flavin-containing monooxygenase family ... 28 6.7
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 27 8.9
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 27 8.9
At2g46710.1 68415.m05828 rac GTPase activating protein, putative... 27 8.9
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 159 bits (386), Expect = 2e-39
Identities = 80/180 (44%), Positives = 100/180 (55%), Gaps = 2/180 (1%)
Frame = +3
Query: 3 KPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXX 182
+PD TY +LIDN + ++G L +DWD LPPKKIKDP AKKPEDW
Sbjct: 180 RPDATYSILIDNVEKQTGSLYSDWDLLPPKKIKDPSAKKPEDWDEQEYISDPEDKKPDGY 239
Query: 183 XXX-XHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWIHPEID 359
IPD D+ K I NP+Y G W PKQI NP YKG W P ID
Sbjct: 240 DDIPKEIPDTDSKKPEDWDDEEDGEWTAPTIPNPEYMGEWKPKQIKNPNYKGKWEAPLID 299
Query: 360 NPEYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE-KGEIIKKMQEGEK 536
NP++ D LY ++ VGL+LWQVKSG++F+N L DDP AK+ E K+++ EK
Sbjct: 300 NPDFKDDPELYVFPKLKYVGLELWQVKSGSLFDNVLICDDPDYAKKLADETWGKLKDAEK 359
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 158 bits (384), Expect = 3e-39
Identities = 77/169 (45%), Positives = 95/169 (56%), Gaps = 1/169 (0%)
Frame = +3
Query: 3 KPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXX 182
+PD TY +LIDN + ++G L +DWD LP KKIKDP AKKPEDW
Sbjct: 180 RPDATYSILIDNVEKQTGSLYSDWDLLPAKKIKDPSAKKPEDWDDKEYIPDPEDTKPAGY 239
Query: 183 XXX-XHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWIHPEID 359
IPD DA K I NP+Y G W PK+I NPAYKG W P ID
Sbjct: 240 DDIPKEIPDTDAKKPEDWDDEEDGEWTAPTIPNPEYNGEWKPKKIKNPAYKGKWKAPMID 299
Query: 360 NPEYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKEKGE 506
NPE+ D LY ++ VG++LWQVKSG++F+N L +DDP AK+ E
Sbjct: 300 NPEFKDDPELYVFPKLKYVGVELWQVKSGSLFDNVLVSDDPEYAKKLAE 348
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 136 bits (330), Expect = 1e-32
Identities = 71/182 (39%), Positives = 95/182 (52%), Gaps = 2/182 (1%)
Frame = +3
Query: 3 KPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXX 182
+PD +Y VL+DN++ E G + DWD LPP+KIK AKKPEDW
Sbjct: 186 RPDASYSVLVDNKEREFGSMYTDWDILPPRKIKVKNAKKPEDWDDREYIDDPNDVKPEGF 245
Query: 183 XXX-XHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWIHPEID 359
IPD A + I N YKG W K+I NP YKG W +P ID
Sbjct: 246 DSIPREIPDRKAKEPEDWDEEENGLWEPPKIPNSAYKGPWKAKRIKNPNYKGKWKNPWID 305
Query: 360 NPEYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE-KGEIIKKMQEGEK 536
NPE+ D +LY I G+++WQVK+G+IF+N L DDPA A+ + + +E EK
Sbjct: 306 NPEFEDDPDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARSIVDDYFAQHRESEK 365
Query: 537 KM 542
++
Sbjct: 366 EL 367
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 133 bits (321), Expect = 1e-31
Identities = 70/180 (38%), Positives = 92/180 (51%), Gaps = 2/180 (1%)
Frame = +3
Query: 9 DNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXX 188
D Y VL+DN++ E G + DWD LPP+KIK AKKPEDW
Sbjct: 134 DTPYSVLVDNKEREFGSMYTDWDILPPRKIKVKNAKKPEDWDDREYIDDPNDVKPEGFDS 193
Query: 189 X-XHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWIHPEIDNP 365
IPD A + I N YKG W K+I NP YKG W +P IDNP
Sbjct: 194 IPREIPDRKAKEPEDWDEEENGLWEPPKIPNSAYKGPWKAKRIKNPNYKGKWKNPWIDNP 253
Query: 366 EYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE-KGEIIKKMQEGEKKM 542
E+ D +LY I G+++WQVK+G+IF+N L DDPA A+ + + +E EK++
Sbjct: 254 EFEDDPDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARSIVDDYFAQHRESEKEL 313
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 71.3 bits (167), Expect = 6e-13
Identities = 47/164 (28%), Positives = 69/164 (42%)
Frame = +3
Query: 3 KPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXX 182
KPD+ E + E E W P +++DPEA KPEDW
Sbjct: 249 KPDDWDEDAPMEIEDEEAEKPEGWLDDEPVEVEDPEASKPEDWDDEEDGEWEA------- 301
Query: 183 XXXXHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWIHPEIDN 362
P S NP YKG W+ IDNPAYKG W +I N
Sbjct: 302 --------PKVSNTKCEAAPGCGEWKRPMKRNPAYKGKWSSPLIDNPAYKGIWKPRDIPN 353
Query: 363 PEYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAK 494
P+Y + + I ++G+++W ++ G +F+N L + D +A+
Sbjct: 354 PDYF-ELERPNLEPIAAIGIEIWTMQDGILFDNILISKDEKVAE 396
Score = 46.8 bits (106), Expect = 1e-05
Identities = 23/47 (48%), Positives = 28/47 (59%), Gaps = 4/47 (8%)
Frame = +3
Query: 3 KPDNTYEVLIDNEKVESGELEADWDFLPP----KKIKDPEAKKPEDW 131
K DN +L+D E+ + G L + DF PP K I DPE KKPEDW
Sbjct: 190 KSDNEVRILVDGEEKKKGNLLSAEDFEPPLIPSKTIPDPEDKKPEDW 236
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 66.1 bits (154), Expect = 2e-11
Identities = 28/73 (38%), Positives = 45/73 (61%)
Frame = +3
Query: 276 NPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSNLYKRDEICSVGLDLWQVKSGTIF 455
NP YKG W+ IDNPAYKG W +I NP+Y + + + I ++G+++W ++ G +F
Sbjct: 323 NPAYKGKWSSPLIDNPAYKGIWKPRDIPNPDYF-ELDRPDYEPIAAIGIEIWTMQDGILF 381
Query: 456 NNFLFTDDPALAK 494
+N L D +A+
Sbjct: 382 DNILIAKDEKVAE 394
>At2g17150.1 68415.m01980 RWP-RK domain-containing protein similar
to nodule inception protein [Lotus japonicus]
GI:6448579; contains Pfam profile: PF02042 RWP-RK domain
Length = 909
Score = 25.8 bits (54), Expect(2) = 0.41
Identities = 8/27 (29%), Positives = 14/27 (51%)
Frame = +3
Query: 294 VWAPKQIDNPAYKGPWIHPEIDNPEYT 374
+W P Q + ++ W P + N EY+
Sbjct: 148 IWVPIQQEGKSFLTTWAQPHLFNQEYS 174
Score = 24.6 bits (51), Expect(2) = 0.41
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +3
Query: 363 PEYTPDSNLYKRDE 404
PE+TPD ++RDE
Sbjct: 210 PEWTPDVRFFRRDE 223
>At4g38190.1 68417.m05391 cellulose synthase family protein similar
to cellulose synthase catalytic subunit gi:2827143 from
[Arabidopsis thaliana], cellulose synthase-5
(gi:9622882) from Zea mays
Length = 1111
Score = 31.1 bits (67), Expect = 0.72
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = -3
Query: 287 VVGVVDHRWFPLSIHLIIPIFRLTGIRVRNVLRFVPVLGFLVFRI 153
+V D W PLS + IP ++ R+ V+RFV + FL +RI
Sbjct: 246 MVETADKPWRPLSRRIPIPAAIISPYRLLIVIRFVVLCFFLTWRI 290
>At3g21330.1 68416.m02694 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 373
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/54 (29%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 109 KQRNLKIGTTNPQSPIRKTRNPRTGTNLSTFRTLMPVSLKIGMMRWM-ESGNHL 267
K++N+KI +T+PQ+ + R R + +TL+P K+ + E+ N+L
Sbjct: 269 KRKNVKI-STDPQTVAARQRRERISEKIRVLQTLVPGGTKMDTASMLDEAANYL 321
>At1g30240.1 68414.m03699 PELP1-related contains weak similarity to
PELP1 [Homo sapiens] gi|21426922|gb|AAC17708
Length = 873
Score = 29.1 bits (62), Expect = 2.9
Identities = 17/57 (29%), Positives = 24/57 (42%)
Frame = +1
Query: 187 NLSTFRTLMPVSLKIGMMRWMESGNHL*STTPTTRASGHLNRLTTLHTRDHGSTRRL 357
+L TL+ + +G W ES ++L TT T G T H + ST L
Sbjct: 564 SLEALETLLTIGGALGSDSWRESVDNLLLTTATNACEGRWANAETYHCLPNKSTTDL 620
>At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 258
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/52 (28%), Positives = 23/52 (44%)
Frame = +1
Query: 109 KQRNLKIGTTNPQSPIRKTRNPRTGTNLSTFRTLMPVSLKIGMMRWMESGNH 264
K R K T+PQS + R + L T + L+P K+ + +E H
Sbjct: 166 KTRATKGTATDPQSLYARKRREKINERLKTLQNLVPNGTKVDISTMLEEAVH 217
>At5g38396.1 68418.m04641 F-box family protein contains F-box domain
Pfam:PF00646
Length = 462
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/89 (26%), Positives = 41/89 (46%), Gaps = 9/89 (10%)
Frame = -3
Query: 353 LRVDPWSLVCRVVNLFRCPDALVVGVVDHRW-----FPLSIHLIIPIF--RLTGIRVRNV 195
LR P L+C +++ +A + V+ RW F ++H+ IF G R RN
Sbjct: 4 LRNIPDELICHILSFLTTKEAALTSVLSKRWRNLLAFVSNLHIDDSIFLHPEEGKRDRNE 63
Query: 194 LR--FVPVLGFLVFRIGDCGFVVPIFRFL 114
+R F+ +G ++ G+C +FL
Sbjct: 64 IRQSFLDFVGRILALQGNCPVKKVSIKFL 92
>At1g78810.2 68414.m09186 expressed protein
Length = 480
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 228 LQAYWHQGPECAQVCPSPRVSCLPD 154
L + WHQGP + PSP + + D
Sbjct: 17 LHSLWHQGPPTRKPIPSPNFNLIHD 41
>At1g78810.1 68414.m09185 expressed protein
Length = 481
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 228 LQAYWHQGPECAQVCPSPRVSCLPD 154
L + WHQGP + PSP + + D
Sbjct: 17 LHSLWHQGPPTRKPIPSPNFNLIHD 41
>At1g12130.1 68414.m01405 flavin-containing monooxygenase family
protein / FMO family protein contains similarity to
flavin-containing monooxygenase 2 (FMO2) from Homo
sapiens [GI:1834493]; contains Pfam profile PF00743
Flavin-binding monooxygenase-like
Length = 470
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/18 (61%), Positives = 14/18 (77%), Gaps = 1/18 (5%)
Frame = -2
Query: 150 GLWVCRPNLQVSLL-LDP 100
GLWVC PN++ LL +DP
Sbjct: 47 GLWVCTPNVEPDLLSIDP 64
>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2
protein, Xenopus laevis, PIR:T30335
Length = 1229
Score = 27.5 bits (58), Expect = 8.9
Identities = 9/20 (45%), Positives = 18/20 (90%)
Frame = +3
Query: 486 LAKEKGEIIKKMQEGEKKMM 545
L KEKG+++K+++EGE+ ++
Sbjct: 580 LQKEKGDLMKEIEEGERDIL 599
>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 7 AMPBP7 (AMPBP7) GI:20799722
Length = 569
Score = 27.5 bits (58), Expect = 8.9
Identities = 20/62 (32%), Positives = 27/62 (43%)
Frame = -1
Query: 487 RAGSSVKRKLLKMVPDLTCHKSRPTEHISSRLYRFESGVYSGLSISGWIHGPLYAGLSIC 308
+AGSS KR LL ++ D TC +S +E + +G W P SI
Sbjct: 143 KAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPW-QPPADEWQSIA 201
Query: 307 LG 302
LG
Sbjct: 202 LG 203
>At2g46710.1 68415.m05828 rac GTPase activating protein, putative
similar to rac GTPase activating protein 2 [Lotus
japonicus] GI:3695061; contains Pfam profiles PF00620:
RhoGAP domain, PF00786: P21-Rho-binding domain
Length = 455
Score = 27.5 bits (58), Expect = 8.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 273 DNPDYKGVWAPKQIDNPAYKGPWIHPEIDNPEYTPDSN 386
+N D K W KQ +P+ + H EI +PE P++N
Sbjct: 331 ENADAKARWLKKQTSDPSEEWESQHSEILSPE-KPNNN 367
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,537,297
Number of Sequences: 28952
Number of extensions: 258451
Number of successful extensions: 823
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 765
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 797
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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