Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_T7_D24
(797 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 301 3e-82
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 74 1e-13
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 74 1e-13
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 40 0.003
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 39 0.003
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 38 0.010
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 37 0.018
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 37 0.018
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 36 0.024
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 36 0.024
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 34 0.095
At2g44760.1 68415.m05571 expressed protein 29 2.7
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 29 3.6
At1g16410.2 68414.m01962 cytochrome P450, putative similar to gb... 29 3.6
At1g16410.1 68414.m01963 cytochrome P450, putative similar to gb... 29 3.6
At5g47010.1 68418.m05794 RNA helicase, putative similar to type ... 28 6.2
At5g45720.1 68418.m05621 hypothetical protein 28 6.2
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 301 bits (739), Expect = 3e-82
Identities = 139/212 (65%), Positives = 166/212 (78%)
Frame = -3
Query: 759 DILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 580
++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPVF T +GKIAVNIC+
Sbjct: 191 EVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICY 250
Query: 579 GRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFTAAINRVGYE 400
GRHH LNW+ FG NGAEIVFNPSAT+ GE SE MW +EARNAAI N YF +INRVG E
Sbjct: 251 GRHHPLNWLAFGLNGAEIVFNPSATV-GE-LSEPMWPIEARNAAIANSYFVGSINRVGTE 308
Query: 399 EFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQIRD 220
FPN FTS DGKP H D G FYGSS+F PD P LSR +DGLLI+ +DLNL RQ +D
Sbjct: 309 VFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDLNLCRQYKD 368
Query: 219 RRCYYMTQRLDMYVNSLSKVLELDYKPQVVHE 124
+ + MT R ++Y + L+K ++ D+KPQVV +
Sbjct: 369 KWGFRMTARYEVYADLLAKYIKPDFKPQVVSD 400
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 73.7 bits (173), Expect = 1e-13
Identities = 55/195 (28%), Positives = 87/195 (44%), Gaps = 5/195 (2%)
Frame = -3
Query: 750 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRH 571
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ +
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQW 190
Query: 570 HVLNWMMFGQNGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCYFTAAINRVG 406
GAEI+F P+A I E + + W + A N A NR+G
Sbjct: 191 FPEAARAMVLQGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIG 249
Query: 405 YEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQI 226
E + P+ FYG+S+ GP G + +L+A DL++ +
Sbjct: 250 -----KEIIETEHGPSQI---TFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSK 301
Query: 225 RDRRCYYMTQRLDMY 181
R + +R D+Y
Sbjct: 302 RQSWGVFRDRRPDLY 316
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 73.7 bits (173), Expect = 1e-13
Identities = 55/195 (28%), Positives = 87/195 (44%), Gaps = 5/195 (2%)
Frame = -3
Query: 750 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRH 571
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ +
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQW 163
Query: 570 HVLNWMMFGQNGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCYFTAAINRVG 406
GAEI+F P+A I E + + W + A N A NR+G
Sbjct: 164 FPEAARAMVLQGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIG 222
Query: 405 YEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQI 226
E + P+ FYG+S+ GP G + +L+A DL++ +
Sbjct: 223 -----KEIIETEHGPSQI---TFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSK 274
Query: 225 RDRRCYYMTQRLDMY 181
R + +R D+Y
Sbjct: 275 RQSWGVFRDRRPDLY 289
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 39.5 bits (88), Expect = 0.003
Identities = 34/117 (29%), Positives = 46/117 (39%), Gaps = 5/117 (4%)
Frame = -3
Query: 759 DILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRYGKIA 595
D L+NT V G + KHRK H IP F ES G T + T G+I
Sbjct: 180 DRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDVGRIG 238
Query: 594 VNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFTA 424
+ IC+ M++ GA ++ P A G W + R A N + A
Sbjct: 239 IGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTG--PLHWELLQRARATDNQLYVA 293
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 39.1 bits (87), Expect = 0.003
Identities = 28/89 (31%), Positives = 38/89 (42%), Gaps = 5/89 (5%)
Frame = -3
Query: 759 DILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRYGKIA 595
D L+NT V G + KHRK H IP F ES G T + T G+I
Sbjct: 180 DRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDVGRIG 238
Query: 594 VNICFGRHHVLNWMMFGQNGAEIVFNPSA 508
+ IC+ M++ GA ++ P A
Sbjct: 239 IGICYDIRFQELAMIYAARGAHLLCYPGA 267
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 37.5 bits (83), Expect = 0.010
Identities = 33/118 (27%), Positives = 54/118 (45%), Gaps = 7/118 (5%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRYGKIAVN 589
L NT VVI D G + ++K H +P + ES++ + G T + G++ +
Sbjct: 128 LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESSFTVPG-TKIVSVDSPVGRLGLT 186
Query: 588 ICFG-RHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAI-TNCYFTAA 421
+C+ R + + + A+++ PSA G E W + R AI T CY AA
Sbjct: 187 VCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTG--EAHWEILLRARAIETQCYVIAA 242
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 36.7 bits (81), Expect = 0.018
Identities = 44/175 (25%), Positives = 67/175 (38%), Gaps = 5/175 (2%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGR 574
L+ T + S G +GKHRK +P + + PV+ T GK+ IC+
Sbjct: 11 LYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWEN 67
Query: 573 HHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEE 397
L G E+ P+A +G E W + AI C+ +A +
Sbjct: 68 RMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCQRKH 121
Query: 396 FPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 244
FP+ FT H + + G S P G G + +GL+ A +DL
Sbjct: 122 FPDHPDYLFTDWYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 175
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 36.7 bits (81), Expect = 0.018
Identities = 44/175 (25%), Positives = 67/175 (38%), Gaps = 5/175 (2%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGR 574
L+ T + S G +GKHRK +P + + PV+ T GK+ IC+
Sbjct: 133 LYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWEN 189
Query: 573 HHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEE 397
L G E+ P+A +G E W + AI C+ +A +
Sbjct: 190 RMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCQRKH 243
Query: 396 FPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 244
FP+ FT H + + G S P G G + +GL+ A +DL
Sbjct: 244 FPDHPDYLFTDWYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 297
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 36.3 bits (80), Expect = 0.024
Identities = 46/175 (26%), Positives = 65/175 (37%), Gaps = 5/175 (2%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGR 574
L+ TA+ S G +GKHRK +P + + PV+ T GKI IC+
Sbjct: 133 LYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGAAICWEN 189
Query: 573 HHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEE 397
L G EI P+A + E W + A+ C+ +A E
Sbjct: 190 RMPLYRTALYAKGIEIYCAPTADYSLE------WQASMIHIAVEGGCFVLSAHQFCKRRE 243
Query: 396 FPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 244
FP F H D + G S P G G + +GL+ A +DL
Sbjct: 244 FPEHPDYLFNDIVDTKEH-DPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDL 297
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 36.3 bits (80), Expect = 0.024
Identities = 44/174 (25%), Positives = 69/174 (39%), Gaps = 4/174 (2%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGR 574
L+ TA+ S G +GKHRK +P + + PV+ T GK+ IC+
Sbjct: 126 LYCTALFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWEN 182
Query: 573 HHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEE 397
L G E+ P+A +G E W + AI C+ +A ++
Sbjct: 183 RMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCLRKD 236
Query: 396 FPN--EFTSADG-KPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 244
FP+ ++ D D + G S P G G + +GL+ A +DL
Sbjct: 237 FPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDL 290
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 34.3 bits (75), Expect = 0.095
Identities = 47/175 (26%), Positives = 69/175 (39%), Gaps = 5/175 (2%)
Frame = -3
Query: 753 LWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF-G 577
L+ T + G +GKHRK +P + + +G+T PVF T GKI IC+
Sbjct: 144 LYCTVLFFDSQGLFLGKHRKL-MPTALERCIWGFG-DGST-IPVFDTPIGKIGAAICWEN 200
Query: 576 RHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFTAAIN----RV 409
R L M+ + G EI P+A S W + A+ F + N R
Sbjct: 201 RMPSLRTAMYAK-GIEIYCAPTA------DSRETWLASMTHIALEGGCFVLSANQFCRRK 253
Query: 408 GYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 244
Y P S + D + G S P G+ G + + L+ A +DL
Sbjct: 254 DYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDL 308
>At2g44760.1 68415.m05571 expressed protein
Length = 504
Score = 29.5 bits (63), Expect = 2.7
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 2/80 (2%)
Frame = -3
Query: 759 DILWNTAVVISDTGNVI-GKHRKNHIP-RVGDFNESNYYMEGNTGHPVFATRYGKIAVNI 586
D + N V++ D ++ G + + + VG+ +ESNYY+ P F ++ + V
Sbjct: 262 DSMINDVVMVFDVADMQDGSYSRGPVTIPVGEMSESNYYLT-----PTF--KFEQCLVKG 314
Query: 585 CFGRHHVLNWMMFGQNGAEI 526
C R V++ + F GA+I
Sbjct: 315 CHKRLRVVHTIEFANGGADI 334
>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich protein APG from
Brassica napus (SP|P40603 ), Arabidopsis thaliana
(GI:22599); contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 29.1 bits (62), Expect = 3.6
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = +2
Query: 437 QFVIAAFLASTFHMYSLP---PSPAIVADGLKTISAPFCPNIIQ--FKTWCRP 580
+F+ +FL S ++ Y L PS V L +++P CPN+ T C+P
Sbjct: 271 KFIKTSFLYSDYYNYMLGLRGPSSNQVGSSLLNVTSPCCPNVYDGGQLTSCKP 323
>At1g16410.2 68414.m01962 cytochrome P450, putative similar to
gb|AF069494 cytochrome P450 from Sinapis alba and is a
member of the PF|00067 Cytochrome P450 family
Length = 423
Score = 29.1 bits (62), Expect = 3.6
Identities = 25/104 (24%), Positives = 43/104 (41%), Gaps = 17/104 (16%)
Frame = -3
Query: 609 YG-KIAVNICFGRHHVLNWMMFGQNG---------AEIVFNPSATIAGEGGSEYM----- 475
YG + + + FGR HV +F +G E++FN + ++Y+
Sbjct: 194 YGYAVTMRMLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLR 253
Query: 474 -WNVEARNAAIT-NCYFTAAINRVGYEEFPNEFTSADGKPAHKD 349
WNV+ + +T NC + N +E + GK A +D
Sbjct: 254 GWNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVED 297
>At1g16410.1 68414.m01963 cytochrome P450, putative similar to
gb|AF069494 cytochrome P450 from Sinapis alba and is a
member of the PF|00067 Cytochrome P450 family
Length = 538
Score = 29.1 bits (62), Expect = 3.6
Identities = 25/104 (24%), Positives = 43/104 (41%), Gaps = 17/104 (16%)
Frame = -3
Query: 609 YG-KIAVNICFGRHHVLNWMMFGQNG---------AEIVFNPSATIAGEGGSEYM----- 475
YG + + + FGR HV +F +G E++FN + ++Y+
Sbjct: 194 YGYAVTMRMLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLR 253
Query: 474 -WNVEARNAAIT-NCYFTAAINRVGYEEFPNEFTSADGKPAHKD 349
WNV+ + +T NC + N +E + GK A +D
Sbjct: 254 GWNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVED 297
>At5g47010.1 68418.m05794 RNA helicase, putative similar to type 1
RNA helicase pNORF1 [Homo sapiens] GI:1885356
Length = 1254
Score = 28.3 bits (60), Expect = 6.2
Identities = 19/66 (28%), Positives = 36/66 (54%), Gaps = 8/66 (12%)
Frame = +1
Query: 79 RWSISAWNRLKLLFIFVYD---LRLV----IQLEYFAETVHVHVQALGHVI-TTSVSDLS 234
RW I + F+F + LRLV ++L Y + VH Q++GHVI T+ +++
Sbjct: 351 RWDIGLNKKRVAYFVFPKEENELRLVPGDELRLRYSGDAVHPSWQSVGHVIKLTAQEEVA 410
Query: 235 VQVQVH 252
++++ +
Sbjct: 411 LELRAN 416
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 6.2
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = -3
Query: 735 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 610
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,575,656
Number of Sequences: 28952
Number of extensions: 344346
Number of successful extensions: 1035
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 996
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1028
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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