Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_T7_D09
(818 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 244 6e-65
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 42 4e-04
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 42 4e-04
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 35 0.075
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 35 0.075
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 34 0.13
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.23
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 29 3.7
At5g47010.1 68418.m05794 RNA helicase, putative similar to type ... 28 6.5
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 244 bits (597), Expect = 6e-65
Identities = 122/221 (55%), Positives = 150/221 (67%)
Frame = -3
Query: 786 ERDEKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDT 607
ERD H ++ W TAV+I GN+IGKHRKNHI F MEG+T ++
Sbjct: 184 ERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDF-NESTYYMEGDTGHPVFETV-F 241
Query: 606 ARFAVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFT 427
+ AVNIC+GRHH LNW+ FG NGAEIVFNPSAT+ GE SE MW +EARNAAI N YF
Sbjct: 242 GKIAVNICYGRHHPLNWLAFGLNGAEIVFNPSATV-GEL-SEPMWPIEARNAAIANSYFV 299
Query: 426 AAINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVD 247
+INRVG E FPN FTS DGKP H D G FYGSS+F PD P LSR +DGLLI+ +D
Sbjct: 300 GSINRVGTEVFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMD 359
Query: 246 LNLNRQIRDRRCYYMTQRLDMYVNSLSKVLELDYKPQVVHE 124
LNL RQ +D+ + MT R ++Y + L+K ++ D+KPQVV +
Sbjct: 360 LNLCRQYKDKWGFRMTARYEVYADLLAKYIKPDFKPQVVSD 400
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 42.3 bits (95), Expect = 4e-04
Identities = 47/204 (23%), Positives = 85/204 (41%), Gaps = 5/204 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
E+ + + + +I G +G +RK+HI + + + + G+T ++ + A+
Sbjct: 124 EEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGY-QEKFYFNPGDTGFKVF-QTKFAKI 181
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCY 433
V IC+ + GAEI+F P+A I E + + W + A N
Sbjct: 182 GVAICWDQWFPEAARAMVLQGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVV 240
Query: 432 FTAAINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAA 253
A NR+G E + P+ FYG+S+ GP G + +L+A
Sbjct: 241 PLVASNRIG-----KEIIETEHGPSQIT---FYGTSFIAGPTGEIVAEADDKSEAVLVAQ 292
Query: 252 VDLNLNRQIRDRRCYYMTQRLDMY 181
DL++ + R + +R D+Y
Sbjct: 293 FDLDMIKSKRQSWGVFRDRRPDLY 316
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 42.3 bits (95), Expect = 4e-04
Identities = 47/204 (23%), Positives = 85/204 (41%), Gaps = 5/204 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
E+ + + + +I G +G +RK+HI + + + + G+T ++ + A+
Sbjct: 97 EEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGY-QEKFYFNPGDTGFKVF-QTKFAKI 154
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCY 433
V IC+ + GAEI+F P+A I E + + W + A N
Sbjct: 155 GVAICWDQWFPEAARAMVLQGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVV 213
Query: 432 FTAAINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAA 253
A NR+G E + P+ FYG+S+ GP G + +L+A
Sbjct: 214 PLVASNRIG-----KEIIETEHGPSQIT---FYGTSFIAGPTGEIVAEADDKSEAVLVAQ 265
Query: 252 VDLNLNRQIRDRRCYYMTQRLDMY 181
DL++ + R + +R D+Y
Sbjct: 266 FDLDMIKSKRQSWGVFRDRRPDLY 289
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 34.7 bits (76), Expect = 0.075
Identities = 46/183 (25%), Positives = 69/183 (37%), Gaps = 5/183 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
EK + T + S G +GKHRK + R Q +G+T P+ Y P +
Sbjct: 5 EKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCIWGQG---DGSTIPV-YDTP-IGKL 59
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAA 421
IC+ L G E+ P+A +G E W + AI C+ +A
Sbjct: 60 GAAICWENRMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSA 113
Query: 420 INRVGYEEFPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAA 253
+ FP+ FT H + + G S P G G + +GL+ A
Sbjct: 114 CQFCQRKHFPDHPDYLFTDWYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTAD 172
Query: 252 VDL 244
+DL
Sbjct: 173 IDL 175
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 34.7 bits (76), Expect = 0.075
Identities = 46/183 (25%), Positives = 69/183 (37%), Gaps = 5/183 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
EK + T + S G +GKHRK + R Q +G+T P+ Y P +
Sbjct: 127 EKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCIWGQG---DGSTIPV-YDTP-IGKL 181
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAA 421
IC+ L G E+ P+A +G E W + AI C+ +A
Sbjct: 182 GAAICWENRMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSA 235
Query: 420 INRVGYEEFPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAA 253
+ FP+ FT H + + G S P G G + +GL+ A
Sbjct: 236 CQFCQRKHFPDHPDYLFTDWYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTAD 294
Query: 252 VDL 244
+DL
Sbjct: 295 IDL 297
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 33.9 bits (74), Expect = 0.13
Identities = 46/182 (25%), Positives = 71/182 (39%), Gaps = 4/182 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
EK + TA+ S G +GKHRK + R Q +G+T P+ Y P +
Sbjct: 120 EKDGYTLYCTALFFSPQGQFLGKHRKLMPTSLERCIWGQG---DGSTIPV-YDTP-IGKL 174
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAA 421
IC+ L G E+ P+A +G E W + AI C+ +A
Sbjct: 175 GAAICWENRMPLYRTALYAKGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSA 228
Query: 420 INRVGYEEFPN--EFTSADG-KPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAV 250
++FP+ ++ D D + G S P G G + +GL+ A +
Sbjct: 229 CQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADL 288
Query: 249 DL 244
DL
Sbjct: 289 DL 290
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 33.1 bits (72), Expect = 0.23
Identities = 47/183 (25%), Positives = 67/183 (36%), Gaps = 5/183 (2%)
Frame = -3
Query: 777 EKHSDIXWXTAVVISXTGNVIGKHRKNHISESRRF*RIQXTXMEGNTXPILYSRPDTARF 598
EK + TA+ S G +GKHRK + R Q +G+T P+ Y P +
Sbjct: 127 EKDGYTLYCTALFFSPQGQFLGKHRKVMPTSLERCIWGQG---DGSTIPV-YDTP-IGKI 181
Query: 597 AVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAA 421
IC+ L G EI P+A + E W + A+ C+ +A
Sbjct: 182 GAAICWENRMPLYRTALYAKGIEIYCAPTADYSLE------WQASMIHIAVEGGCFVLSA 235
Query: 420 INRVGYEEFPNE----FTSADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAA 253
EFP F H D + G S P G G + +GL+ A
Sbjct: 236 HQFCKRREFPEHPDYLFNDIVDTKEH-DPTVSGGGSVIISPLGKVLAGPNYESEGLVTAD 294
Query: 252 VDL 244
+DL
Sbjct: 295 LDL 297
>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich protein APG from
Brassica napus (SP|P40603 ), Arabidopsis thaliana
(GI:22599); contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 29.1 bits (62), Expect = 3.7
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = +2
Query: 437 QFVIAAFLASTFHMYSLP---PSPAIVADGLKTISAPFCPNIIQ--FKTWCRP 580
+F+ +FL S ++ Y L PS V L +++P CPN+ T C+P
Sbjct: 271 KFIKTSFLYSDYYNYMLGLRGPSSNQVGSSLLNVTSPCCPNVYDGGQLTSCKP 323
>At5g47010.1 68418.m05794 RNA helicase, putative similar to type 1
RNA helicase pNORF1 [Homo sapiens] GI:1885356
Length = 1254
Score = 28.3 bits (60), Expect = 6.5
Identities = 19/66 (28%), Positives = 36/66 (54%), Gaps = 8/66 (12%)
Frame = +1
Query: 79 RWSISAWNRLKLLFIFVYD---LRLV----IQLEYFAETVHVHVQALGHVI-TTSVSDLS 234
RW I + F+F + LRLV ++L Y + VH Q++GHVI T+ +++
Sbjct: 351 RWDIGLNKKRVAYFVFPKEENELRLVPGDELRLRYSGDAVHPSWQSVGHVIKLTAQEEVA 410
Query: 235 VQVQVH 252
++++ +
Sbjct: 411 LELRAN 416
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,973,481
Number of Sequences: 28952
Number of extensions: 315077
Number of successful extensions: 899
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 893
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1872844800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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