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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0021
         (611 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep: ...    74   3e-12
UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit R...    72   1e-11
UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST neig...    71   3e-11
UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome s...    64   2e-09
UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit R...    62   8e-09
UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella ve...    56   7e-07
UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep: CG1377...    50   3e-05
UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1; ...    50   3e-05
UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gamb...    49   8e-05
UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of str...    39   0.081
UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit R...    37   0.33 
UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albic...    36   0.57 
UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2; ...    36   1.0  
UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit ...    36   1.0  
UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;...    34   2.3  
UniRef50_A0CSJ2 Cluster: Chromosome undetermined scaffold_26, wh...    33   4.0  
UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1; Dictyost...    33   7.1  

>UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep:
           LOC443718 protein - Xenopus laevis (African clawed frog)
          Length = 477

 Score = 73.7 bits (173), Expect = 3e-12
 Identities = 34/85 (40%), Positives = 48/85 (56%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y N  ++  +G I +D   IH  V+AD+ IF+P  G  L G+VNK   TH+  LVH  FN
Sbjct: 94  YDNIKLVGELGDIYDDLGHIHINVEADFVIFKPDCGQTLVGIVNKVAPTHIGCLVHGCFN 153

Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
             IP+P + P + W+   VK   E+
Sbjct: 154 ASIPKPPKMPIENWLRIGVKIDDEI 178



 Score = 34.7 bits (76), Expect = 1.8
 Identities = 20/57 (35%), Positives = 33/57 (57%)
 Frame = +1

Query: 85  ELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKELNGVLVS 255
           E  +L + + SCLVV+   ++LAL P  L   +  I   L+ ++ K+ + LNGV V+
Sbjct: 37  EACELVSCRYSCLVVETHRRHLALSPKFLRKKRSGIQEQLNTELLKYSERLNGVPVA 93


>UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit
           RPA43; n=16; Amniota|Rep: DNA-directed RNA polymerase I
           subunit RPA43 - Homo sapiens (Human)
          Length = 338

 Score = 71.7 bits (168), Expect = 1e-11
 Identities = 32/85 (37%), Positives = 48/85 (56%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y N  ++  +G I +D   IH  ++AD+ IF P  G KL G+VNK  S+H+  LVH  FN
Sbjct: 93  YDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSSSHIGCLVHGCFN 152

Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
             IP+P +   ++W    +  G E+
Sbjct: 153 ASIPKPEQLSAEQWQTMEINMGDEL 177


>UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST
           neighbor; n=1; Canis lupus familiaris|Rep: PREDICTED:
           similar to TWIST neighbor - Canis familiaris
          Length = 423

 Score = 70.5 bits (165), Expect = 3e-11
 Identities = 32/85 (37%), Positives = 48/85 (56%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y N  ++  +G I +D   IH  ++AD+ IF P  G KL G+VNK  S+H+  LVH  FN
Sbjct: 171 YDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGKKLMGIVNKVSSSHIGCLVHGCFN 230

Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
             IP+P +   ++W    +  G E+
Sbjct: 231 ASIPKPEQMSTEQWQILEINVGDEL 255


>UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome
           shotgun sequence; n=3; Tetraodontidae|Rep: Chromosome 21
           SCAF15029, whole genome shotgun sequence - Tetraodon
           nigroviridis (Green puffer)
          Length = 352

 Score = 64.5 bits (150), Expect = 2e-09
 Identities = 32/74 (43%), Positives = 40/74 (54%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y N  IL   G I +D   +H  V AD+ +FQP  G KL G VNK   +H+  LVH  FN
Sbjct: 109 YDNIRILGQYGDIVDDGGYVHMDVIADFIVFQPQKGQKLLGKVNKLGVSHVGCLVHGCFN 168

Query: 435 VVIPQPTEEPGDKW 476
             IP+P   P + W
Sbjct: 169 ASIPKPNLVPMETW 182


>UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit
           RPA43; n=3; Danio rerio|Rep: DNA-directed RNA polymerase
           I subunit RPA43 - Danio rerio (Zebrafish) (Brachydanio
           rerio)
          Length = 391

 Score = 62.5 bits (145), Expect = 8e-09
 Identities = 28/85 (32%), Positives = 47/85 (55%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y N  ++   G I +D   IHF ++A + IF+P  G++L G++NK  ++H+  LVH  FN
Sbjct: 101 YDNIKVVGQHGNIYDDQGFIHFNIEASFVIFRPKNGSRLMGVINKMGASHVGCLVHGCFN 160

Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
             + +P     D+W    +  GQ +
Sbjct: 161 ASVMKPNALTSDQWRDSGLCVGQSL 185


>UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 159

 Score = 56.0 bits (129), Expect = 7e-07
 Identities = 28/71 (39%), Positives = 37/71 (52%)
 Frame = +3

Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
           Y    +LQ  G I +++  +HF  Q DY  F+P IG KL G+VNK    H+  LVH  FN
Sbjct: 52  YDKVTLLQRRGNIIDESPFLHFDAQVDYITFKPEIGNKLIGVVNKFGYDHIGCLVHGCFN 111

Query: 435 VVIPQPTEEPG 467
             I +     G
Sbjct: 112 ASIAKSRTSNG 122


>UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep:
           CG13773-PA - Drosophila melanogaster (Fruit fly)
          Length = 253

 Score = 50.4 bits (115), Expect = 3e-05
 Identities = 22/62 (35%), Positives = 37/62 (59%)
 Frame = +3

Query: 258 KNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNV 437
           KN  +L     +R D+  +H  + AD+++F+P  GA L+G+V      H+S +++RVFN 
Sbjct: 74  KNIKVLGQTAGLRADDPTMHLVINADFYVFRPKAGAILSGVVRHISRHHVSAVIYRVFNT 133

Query: 438 VI 443
            I
Sbjct: 134 SI 135



 Score = 48.8 bits (111), Expect = 1e-04
 Identities = 25/71 (35%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
 Frame = +1

Query: 52  IMSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLL-DYKVGKFD 228
           I+ K IKF ++EL+  A+   SC+       +LA+ P+ + N K ++  LL   KVG +D
Sbjct: 4   ILQKYIKFSVKELENYASSPESCVRCITTDMHLAMGPYGMANFKHALHELLIRTKVGFYD 63

Query: 229 KELNGVLVSIK 261
             L+G+++ IK
Sbjct: 64  SGLDGIVLGIK 74


>UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1;
           Aedes aegypti|Rep: Putative uncharacterized protein -
           Aedes aegypti (Yellowfever mosquito)
          Length = 571

 Score = 50.4 bits (115), Expect = 3e-05
 Identities = 22/70 (31%), Positives = 41/70 (58%)
 Frame = +3

Query: 234 VERCLSKYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSV 413
           V+  +  +KN  IL  +  IR ++  IH +V +++++F+P  GA + G +      +LS 
Sbjct: 66  VDGIILAFKNTKILSPLSAIRPNSVRIHVKVSSNFYVFRPISGATIEGTIKYVSKNYLSA 125

Query: 414 LVHRVFNVVI 443
           +++ VFNV I
Sbjct: 126 VIYGVFNVTI 135



 Score = 47.6 bits (108), Expect = 2e-04
 Identities = 21/68 (30%), Positives = 41/68 (60%)
 Frame = +1

Query: 58  SKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKEL 237
           +K  K+ + EL+    D NSC+   + T  +++ P  LG+I E +   L  K+GK+D+ +
Sbjct: 7   TKYAKYTVEELESYVADPNSCVQKLEWTNLVSVLPKYLGDICEGVKACLTQKIGKYDRTV 66

Query: 238 NGVLVSIK 261
           +G++++ K
Sbjct: 67  DGIILAFK 74


>UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gambiae
           str. PEST|Rep: ENSANGP00000016750 - Anopheles gambiae
           str. PEST
          Length = 246

 Score = 49.2 bits (112), Expect = 8e-05
 Identities = 20/54 (37%), Positives = 33/54 (61%)
 Frame = +3

Query: 282 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
           +  +R D+  +H +   DY++FQP IG+ L G VN      +S +++RVFNV +
Sbjct: 82  LSALRLDSPYLHVRTTIDYYVFQPRIGSTLRGTVNYVSKNFVSAVIYRVFNVTV 135



 Score = 39.1 bits (87), Expect = 0.081
 Identities = 17/66 (25%), Positives = 38/66 (57%)
 Frame = +1

Query: 55  MSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKE 234
           ++K  KF   EL++   D  SC+   ++ + L+L+P    +I + +   +  K+G++  +
Sbjct: 6   IAKYTKFSAEELQKSTADPLSCVAAVQLNEILSLRPRDCEHIVKGVKRCVTDKIGRYHAK 65

Query: 235 LNGVLV 252
           LNG+++
Sbjct: 66  LNGIVL 71


>UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of strain
           CLIB122 of Yarrowia lipolytica; n=1; Yarrowia
           lipolytica|Rep: Yarrowia lipolytica chromosome F of
           strain CLIB122 of Yarrowia lipolytica - Yarrowia
           lipolytica (Candida lipolytica)
          Length = 288

 Score = 39.1 bits (87), Expect = 0.081
 Identities = 16/54 (29%), Positives = 29/54 (53%)
 Frame = +3

Query: 282 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
           +G   N++      +  D  +++P +G +L G VN +  +H+ +LVH  FN  I
Sbjct: 98  MGKFVNESPFTFMWISTDLLVWKPQVGDRLEGYVNMQTPSHIGLLVHDTFNCSI 151


>UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit
           RPA43; n=1; Schizosaccharomyces pombe|Rep: DNA-directed
           RNA polymerase I subunit RPA43 - Schizosaccharomyces
           pombe (Fission yeast)
          Length = 173

 Score = 37.1 bits (82), Expect = 0.33
 Identities = 23/83 (27%), Positives = 38/83 (45%)
 Frame = +3

Query: 231 RVERCLSKYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLS 410
           R+   +  Y N   L+    +  D+      V+ D  +F P  G  L G +N    +H+ 
Sbjct: 43  RINGIVLAYDNIRFLEKSAKVMYDSPFSFIWVRVDVLVFSPKKGDCLEGKINLVSPSHIG 102

Query: 411 VLVHRVFNVVIPQPTEEPGDKWI 479
           +L+  +FN  IP+ +  P D WI
Sbjct: 103 LLILGIFNASIPRKS-IPKD-WI 123


>UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albicans
           CaRPA43 DNA-directed RNA polymerase I; n=3;
           Saccharomycetales|Rep: Similar to CA2272|CaRPA43 Candida
           albicans CaRPA43 DNA-directed RNA polymerase I -
           Debaryomyces hansenii (Yeast) (Torulaspora hansenii)
          Length = 311

 Score = 36.3 bits (80), Expect = 0.57
 Identities = 14/40 (35%), Positives = 24/40 (60%)
 Frame = +3

Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
           V  D+ I++P +G  L G +  + ++H+ +LVH  FN  I
Sbjct: 122 VSVDFLIWRPQVGDTLEGYIYMQTASHIGLLVHDTFNASI 161


>UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2;
           Sclerotiniaceae|Rep: Putative uncharacterized protein -
           Botryotinia fuckeliana B05.10
          Length = 323

 Score = 35.5 bits (78), Expect = 1.0
 Identities = 17/58 (29%), Positives = 30/58 (51%)
 Frame = +3

Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQPTEEPGDKWIGFSVKE 497
           V A++ +F+P  GA L G +N +   HL ++   +FN  I +       +W+G   +E
Sbjct: 150 VTAEFLLFKPEKGAWLEGYINLQNEGHLGLVCWNLFNASIERARLPKEWRWVGVEDQE 207


>UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit
           A43; n=2; Pichia|Rep: DNA-dependent RNA polymerase I
           subunit A43 - Pichia stipitis (Yeast)
          Length = 307

 Score = 35.5 bits (78), Expect = 1.0
 Identities = 13/40 (32%), Positives = 24/40 (60%)
 Frame = +3

Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
           +  D+ I++P +G  L G +  + ++HL +L+H  FN  I
Sbjct: 122 ITVDFLIWRPQVGDVLEGYIYMQTASHLGLLIHDTFNASI 161


>UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;
           Ostreococcus|Rep: DNA polymerase epsilon, subunit C -
           Ostreococcus tauri
          Length = 569

 Score = 34.3 bits (75), Expect = 2.3
 Identities = 18/53 (33%), Positives = 27/53 (50%)
 Frame = +3

Query: 285 GTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
           G + + +  +   V+AD  +F P  G++L G VNK     +  LV   FNV I
Sbjct: 101 GRVLHASGHVDVCVEADCDVFTPVRGSRLVGTVNKIAHDFIGALVLDKFNVAI 153


>UniRef50_A0CSJ2 Cluster: Chromosome undetermined scaffold_26, whole
           genome shotgun sequence; n=1; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_26,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 678

 Score = 33.5 bits (73), Expect = 4.0
 Identities = 25/89 (28%), Positives = 45/89 (50%), Gaps = 2/89 (2%)
 Frame = +1

Query: 64  IIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKV--GKFDKEL 237
           + +FD+  ++     K   L++ K T N+ LQ +C+  I   I  LL YK    KF KE 
Sbjct: 124 LTEFDIFIIRVQCLYKWGKLIMIKYTNNINLQRFCIQRILTEIKPLLHYKYFDEKFQKES 183

Query: 238 NGVLVSIKILVYFRMLALYAMIMRIYISK 324
             +L  I+  +  +  ++YA+ +   ++K
Sbjct: 184 LNLLAKIREWL-AKEQSIYALCLSYKLTK 211


>UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1;
           Dictyostelium discoideum AX4|Rep: RNA polymerase I
           subunit - Dictyostelium discoideum AX4
          Length = 356

 Score = 32.7 bits (71), Expect = 7.1
 Identities = 13/50 (26%), Positives = 27/50 (54%)
 Frame = +3

Query: 300 DNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQ 449
           D+  +   +   + +F+P+    L G+V +  +TH+S+LV    +  IP+
Sbjct: 79  DSPYLGMNIHVKFLVFKPFKDQILNGVVKRVSTTHISLLVFGTISASIPK 128


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 542,570,583
Number of Sequences: 1657284
Number of extensions: 9996755
Number of successful extensions: 21515
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 21044
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21512
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 43977329078
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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