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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0086
         (686 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo...    45   0.002
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ...    36   0.93 
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j...    36   0.93 
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ...    36   1.2  
UniRef50_Q5KL82 Cluster: Putative uncharacterized protein; n=1; ...    35   1.6  
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet...    34   3.7  
UniRef50_UPI000049A56F Cluster: protein kinase; n=1; Entamoeba h...    33   6.5  
UniRef50_Q3LWH8 Cluster: Putative uncharacterized protein; n=1; ...    33   8.6  
UniRef50_Q13469 Cluster: Nuclear factor of activated T-cells, cy...    33   8.6  

>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
           Eukaryota|Rep: beta-galactosidase - Entamoeba
           histolytica HM-1:IMSS
          Length = 86

 Score = 45.2 bits (102), Expect = 0.002
 Identities = 22/34 (64%), Positives = 26/34 (76%)
 Frame = +1

Query: 571 GKPWRLPNLIALQHIPLLPGWRIAKKARTDRLSK 672
           GK   LPNLIALQHIPL P   I+++ARTDR S+
Sbjct: 15  GKTLALPNLIALQHIPLSPAGVISEEARTDRPSQ 48



 Score = 40.3 bits (90), Expect = 0.043
 Identities = 18/29 (62%), Positives = 21/29 (72%)
 Frame = +3

Query: 540 HWPAFYNVVTGKTLAVTQLNCLAAHSPFA 626
           HWP+FYNVVTGKTLA+  L  L  H P +
Sbjct: 5   HWPSFYNVVTGKTLALPNLIAL-QHIPLS 32


>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
           organisms|Rep: LacZ-alpha peptide - Escherichia coli
          Length = 90

 Score = 35.9 bits (79), Expect = 0.93
 Identities = 15/16 (93%), Positives = 15/16 (93%)
 Frame = +2

Query: 536 NSLAGVLQRRDWENPG 583
           NSLA VLQRRDWENPG
Sbjct: 20  NSLAVVLQRRDWENPG 35


>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
           japonicum|Rep: SJCHGC09076 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 109

 Score = 35.9 bits (79), Expect = 0.93
 Identities = 23/59 (38%), Positives = 28/59 (47%)
 Frame = +1

Query: 496 GGPVPIRPIGVVLQFTGRRFTTS*LGKPWRLPNLIALQHIPLLPGWRIAKKARTDRLSK 672
           G   PI P G   + +G  F     GK    P L  L+ +PL PG    KKA  +RLSK
Sbjct: 40  GARDPISPKGGPNKISGAAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPPNRLSK 98


>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
           Erwinia amylovora|Rep: Putative uncharacterized protein
           - Erwinia amylovora (Fire blight bacteria)
          Length = 123

 Score = 35.5 bits (78), Expect = 1.2
 Identities = 18/23 (78%), Positives = 18/23 (78%)
 Frame = +2

Query: 515 AL*GSYYNSLAGVLQRRDWENPG 583
           AL  SYY  LA VLQRRDWENPG
Sbjct: 60  ALSESYYG-LAVVLQRRDWENPG 81


>UniRef50_Q5KL82 Cluster: Putative uncharacterized protein; n=1;
           Filobasidiella neoformans|Rep: Putative uncharacterized
           protein - Cryptococcus neoformans (Filobasidiella
           neoformans)
          Length = 477

 Score = 35.1 bits (77), Expect = 1.6
 Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
 Frame = -1

Query: 575 FPSHDVVKR-RPVNCNTTPIGRIGTGPPLERPRTV 474
           +PS D+ K  RP+  + T I RI TG PL  PR+V
Sbjct: 293 YPSDDITKPPRPIYASQTAIFRIATGMPLRSPRSV 327


>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
           Beta-galactosidase - Escherichia coli (strain K12)
          Length = 1024

 Score = 33.9 bits (74), Expect = 3.7
 Identities = 14/16 (87%), Positives = 15/16 (93%)
 Frame = +2

Query: 536 NSLAGVLQRRDWENPG 583
           +SLA VLQRRDWENPG
Sbjct: 6   DSLAVVLQRRDWENPG 21


>UniRef50_UPI000049A56F Cluster: protein kinase; n=1; Entamoeba
            histolytica HM-1:IMSS|Rep: protein kinase - Entamoeba
            histolytica HM-1:IMSS
          Length = 1637

 Score = 33.1 bits (72), Expect = 6.5
 Identities = 19/50 (38%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
 Frame = +1

Query: 157  YYVIFLIYNVNRHLSIYLLIIIGLCNKT-FRNKIKKKNY*SGHFV*IECC 303
            ++VI L+  V   ++I +LIIIG C++T +R KI+K+      F  I  C
Sbjct: 1184 WWVIILVILVILSITIIILIIIGWCSRTYYRYKIQKQYEKENSFFNISYC 1233


>UniRef50_Q3LWH8 Cluster: Putative uncharacterized protein; n=1;
           Bigelowiella natans|Rep: Putative uncharacterized
           protein - Bigelowiella natans (Pedinomonas minutissima)
           (Chlorarachnion sp.(strain CCMP 621))
          Length = 291

 Score = 32.7 bits (71), Expect = 8.6
 Identities = 20/60 (33%), Positives = 31/60 (51%)
 Frame = -3

Query: 333 NDLLRFYIIKTTFDLNEMTRLIVFFFYFISKSFITESDYNQQIYT*VTVNIINEKDYIIL 154
           N + +FY IK  F  + + +  + FFYF +KS  T S +    Y    + +IN K Y I+
Sbjct: 206 NTVKKFYSIKDIFGFSLIKKANINFFYFSNKSIKTSSYFIINTYKNFLL-VINIKKYSIV 264


>UniRef50_Q13469 Cluster: Nuclear factor of activated T-cells,
           cytoplasmic 2; n=36; Tetrapoda|Rep: Nuclear factor of
           activated T-cells, cytoplasmic 2 - Homo sapiens (Human)
          Length = 925

 Score = 32.7 bits (71), Expect = 8.6
 Identities = 18/65 (27%), Positives = 25/65 (38%)
 Frame = +3

Query: 486 PLEGGPGTNSPYRGRITIHWPAFYNVVTGKTLAVTQLNCLAAHSPFARLAYSEKGPDRSP 665
           P  GGP    P    I  H+    + +     ++ + +CL  HSP  R A     P    
Sbjct: 193 PNNGGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKR 252

Query: 666 FQSCA 680
             SCA
Sbjct: 253 RHSCA 257


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 582,192,396
Number of Sequences: 1657284
Number of extensions: 10370662
Number of successful extensions: 22205
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 21625
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22201
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53719013270
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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