Silkworm BLAST Result against tribolium

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20027
         (677 letters)

Database: tribolium 
           336 sequences; 122,585 total letters

Searching.......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome ad...    38   8e-05
DQ490059-1|ABF22614.1|  947|Tribolium castaneum short gastrulati...    22   5.3  
AY884061-1|AAX84202.1|  717|Tribolium castaneum laccase 2A protein.    21   7.0  

>DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome
           adhesion molecule splicevariant 3.12.3.1 protein.
          Length = 1639

 Score = 37.9 bits (84), Expect = 8e-05
 Identities = 38/120 (31%), Positives = 54/120 (45%), Gaps = 7/120 (5%)
 Frame = +2

Query: 338 PKPTIQWYK--DGTP----LTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPV 499
           P P+ +WYK  +GT     +T ND  ++V    G L   ++R  K  D G Y CV  N V
Sbjct: 260 PSPSFRWYKFVEGTTRKQAVTLNDRVKQVA---GTL---IIREAKVEDSGKYLCVVNNSV 313

Query: 500 -GEALAKTLRLLLPYSAMNLRSNLTM*K*LVANLLFYNAHHRKGCLIPSIHWNKDGHSLD 676
            GE++   L +  P  A       T+     A        + +G  I +I W KDGH +D
Sbjct: 314 GGESVETVLTVTAPLKAKIEPQVQTIDFGRPATF----TCNFEGNPIKTISWLKDGHPID 369



 Score = 32.7 bits (71), Expect = 0.003
 Identities = 20/64 (31%), Positives = 31/64 (48%)
 Frame = +2

Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAK 517
           P  ++ W +DG  L  N   Q+V +   L+   V R    SD+  Y CVA+N  G +   
Sbjct: 538 PVDSVVWERDGRQLPIN-RKQKVFINGTLIIENVERA---SDQATYDCVAKNSQGYSARG 593

Query: 518 TLRL 529
           +L +
Sbjct: 594 SLEV 597



 Score = 31.1 bits (67), Expect = 0.009
 Identities = 21/52 (40%), Positives = 27/52 (51%)
 Frame = +2

Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARN 493
           P  TI W KDG P+   DH + V        LR+   +KE D+G+Y C  RN
Sbjct: 355 PIKTISWLKDGHPI---DHNEAV--------LRIESVRKE-DKGMYQCFIRN 394



 Score = 30.7 bits (66), Expect = 0.011
 Identities = 20/67 (29%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
 Frame = +2

Query: 338 PKPTIQWYK-DGTPLTSNDHPQRVLLEDGLLF--LRVMRGKKESDEGVYWCVARNPVGEA 508
           P P I W + DGT +      ++VL    L+F   R    ++E    VY C+A+N VG  
Sbjct: 51  PTPDIIWVRSDGTAVGDVPGLRQVLANGNLVFPPFRAEDYRQEVHAQVYVCLAKNSVGSV 110

Query: 509 LAKTLRL 529
            ++ + +
Sbjct: 111 HSRDVNV 117



 Score = 29.9 bits (64), Expect = 0.020
 Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
 Frame = +2

Query: 338 PKPTIQWYKDGTPLTSNDH----PQR--VLLEDGLLFLRVMRGKKESDEGVYWCVARNPV 499
           P+P + W K  T ++  D+    P    + +EDG L +  ++   +++EG Y C A N +
Sbjct: 726 PRPVVTW-KRATGVSPGDYKDFKPNNPDIKVEDGTLTINNIQ---KTNEGYYLCEAVNGI 781

Query: 500 GEALAKTLRL 529
           G  L+  +++
Sbjct: 782 GSGLSAVIQI 791



 Score = 25.0 bits (52), Expect = 0.57
 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
 Frame = +2

Query: 338 PKPTIQWYKDGTPLTSNDHPQ---RVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEA 508
           P P I W   G  L++++  Q    V +   ++    +     +D G+Y CVA + VG A
Sbjct: 441 PTPEITWELYGRRLSNSERNQIGQYVTVNGDVVSHLNITAIHTNDGGLYRCVASSKVGSA 500



 Score = 23.0 bits (47), Expect = 2.3
 Identities = 14/47 (29%), Positives = 20/47 (42%)
 Frame = +1

Query: 502 GSISKNATLTIAVLRDEFKIEPHDVKVASGEPALLQCASPKGVPDSV 642
           GS+        AV+   ++ E     V  G  A+L+C  P  V D V
Sbjct: 108 GSVHSRDVNVRAVVNQYYEAEVVSEYVIRGNTAVLKCNIPSFVADFV 154


>DQ490059-1|ABF22614.1|  947|Tribolium castaneum short gastrulation
           protein.
          Length = 947

 Score = 21.8 bits (44), Expect = 5.3
 Identities = 9/25 (36%), Positives = 18/25 (72%)
 Frame = +2

Query: 365 DGTPLTSNDHPQRVLLEDGLLFLRV 439
           +G+P+TS +  +   L++G+ FL+V
Sbjct: 604 EGSPVTSLNREEIKRLDNGVGFLKV 628


>AY884061-1|AAX84202.1|  717|Tribolium castaneum laccase 2A protein.
          Length = 717

 Score = 21.4 bits (43), Expect = 7.0
 Identities = 8/27 (29%), Positives = 14/27 (51%)
 Frame = -2

Query: 88  CLFLFRSIVYKDIAMHL*NDATQPPPP 8
           C FLF  ++  ++ + +   A  PP P
Sbjct: 671 CHFLFHIVIGMNLVLQVGTHADLPPVP 697



 Score = 21.0 bits (42), Expect = 9.3
 Identities = 11/24 (45%), Positives = 14/24 (58%), Gaps = 3/24 (12%)
 Frame = -3

Query: 216 KIQQYN---KFHLIAYLFNLIRTG 154
           ++QQ N    FHL  Y FN+I  G
Sbjct: 587 EVQQPNLSHPFHLHGYAFNVIGIG 610


  Database: tribolium
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 122,585
  Number of sequences in database:  336
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 168,852
Number of Sequences: 336
Number of extensions: 4093
Number of successful extensions: 13
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13
length of database: 122,585
effective HSP length: 55
effective length of database: 104,105
effective search space used: 17697850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)