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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV30003
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   129   4e-31
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   129   4e-31
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    94   2e-20
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    81   1e-16
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    34   0.017
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces...    27   1.9  
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos...    27   2.6  
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-...    26   4.5  
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ...    26   4.5  
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo...    26   4.5  
SPAC5D6.09c |mug86||acetate transporter |Schizosaccharomyces pom...    25   7.9  
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual     25   7.9  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  129 bits (311), Expect = 4e-31
 Identities = 56/85 (65%), Positives = 66/85 (77%)
 Frame = -2

Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
           +EIQ  E A+GGIY VLN++RGHVF E Q  GTP++ +KAYLPVNESFGFT +LR  T G
Sbjct: 731 VEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAG 790

Query: 331 QAFPQCVFDHWQVLPGDPCEPQSKP 257
           QAFPQ VFDHW  + GDP +P SKP
Sbjct: 791 QAFPQLVFDHWSPMSGDPLDPTSKP 815



 Score = 89.0 bits (211), Expect = 6e-19
 Identities = 42/65 (64%), Positives = 47/65 (72%)
 Frame = -3

Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
           +KEG M EENLR  RFNI DV LH DAIHRGGGQIIPT RR +YA  L A P + EPV+L
Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFL 730

Query: 510 WKFSV 496
            +  V
Sbjct: 731 VEIQV 735



 Score = 31.1 bits (67), Expect = 0.16
 Identities = 16/34 (47%), Positives = 20/34 (58%)
 Frame = -1

Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
           P++G P  P     Q   +V E RKRKGLKE +P
Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  129 bits (311), Expect = 4e-31
 Identities = 56/85 (65%), Positives = 66/85 (77%)
 Frame = -2

Query: 511 LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
           +EIQ  E A+GGIY VLN++RGHVF E Q  GTP++ +KAYLPVNESFGFT +LR  T G
Sbjct: 731 VEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAG 790

Query: 331 QAFPQCVFDHWQVLPGDPCEPQSKP 257
           QAFPQ VFDHW  + GDP +P SKP
Sbjct: 791 QAFPQLVFDHWSPMSGDPLDPTSKP 815



 Score = 89.0 bits (211), Expect = 6e-19
 Identities = 42/65 (64%), Positives = 47/65 (72%)
 Frame = -3

Query: 690 AKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
           +KEG M EENLR  RFNI DV LH DAIHRGGGQIIPT RR +YA  L A P + EPV+L
Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFL 730

Query: 510 WKFSV 496
            +  V
Sbjct: 731 VEIQV 735



 Score = 31.1 bits (67), Expect = 0.16
 Identities = 16/34 (47%), Positives = 20/34 (58%)
 Frame = -1

Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
           P++G P  P     Q   +V E RKRKGLKE +P
Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 983

 Score = 93.9 bits (223), Expect = 2e-20
 Identities = 45/97 (46%), Positives = 63/97 (64%)
 Frame = -2

Query: 511  LEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 332
            +E+  P  ++  IY +L RRRGHV ++    G+P+++V+A +PV +S GF  DLR +T G
Sbjct: 847  VEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSCGFETDLRVHTQG 906

Query: 331  QAFPQCVFDHWQVLPGDPCEPQSKP*TLYRKRERGKD 221
            QA  Q VFDHWQV+PGDP +   KP  L  +  RG D
Sbjct: 907  QAMCQMVFDHWQVVPGDPLDKSIKPKPL--EPARGSD 941



 Score = 75.8 bits (178), Expect = 6e-15
 Identities = 33/59 (55%), Positives = 42/59 (71%)
 Frame = -3

Query: 687 KEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYL 511
           +EG + +E +R V F + DV L  + I+RGGGQIIPT RR  Y+  LTA PRLMEPVY+
Sbjct: 788 REGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFLTASPRLMEPVYM 846



 Score = 27.9 bits (59), Expect = 1.5
 Identities = 15/49 (30%), Positives = 24/49 (48%)
 Frame = -1

Query: 359  CRFAFQHRRTGLPAVRIRPLAGPPWRPVRTSEQALNVVQETRKRKGLKE 213
            C+  F H +        + +   P  P R S+ A + + +TR+RKGL E
Sbjct: 910  CQMVFDHWQVVPGDPLDKSIKPKPLEPARGSDLARDFLIKTRRRKGLVE 958


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1000

 Score = 81.0 bits (191), Expect = 1e-16
 Identities = 35/82 (42%), Positives = 52/82 (63%)
 Frame = -2

Query: 523  ACISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 344
            A  S ++Q     +G +YGV+++RRG V +E    GTP FIVKA +PV ESFGF  ++  
Sbjct: 865  AMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFAVEILK 924

Query: 343  NTGGQAFPQCVFDHWQVLPGDP 278
             T G A+PQ +F  +++L  +P
Sbjct: 925  RTSGAAYPQLIFHGFEMLDENP 946


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 16/63 (25%), Positives = 35/63 (55%)
 Frame = -2

Query: 517 ISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNT 338
           +++ I  P    GG+ G L++R+  + +         F ++A +P+N  F +++D+R+ T
Sbjct: 679 MNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRALT 736

Query: 337 GGQ 329
            G+
Sbjct: 737 KGK 739


>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 324

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 28/95 (29%), Positives = 41/95 (43%), Gaps = 6/95 (6%)
 Frame = -3

Query: 297 RSSLETRANLRASPKRCTGNEKEERIEGRSPRLNSIFGQIVNYIVK-AN*KNVSIRKALH 121
           +SSL T+ N    PK+   N ++ER+E R   +  +  Q      K A+ KN   +K   
Sbjct: 120 QSSLNTKENTPQQPKKSR-NRQKERLERRKAEMKKMSEQAELESEKMADLKNEE-KKKFS 177

Query: 120 ACLFSTTHVPHHVKAD-----AQVRQQLNMSHSTK 31
             L     V   + AD     A +  QLN  H+ K
Sbjct: 178 KILEEAGLVAVDIPADGNCLFASISHQLNYHHNVK 212


>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 731

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = -1

Query: 305 PLAGPPWRPVRTSEQALNVVQETRKRKGLKEGLP 204
           PL+ P  R V  +E ALN++ E  +R  LKE  P
Sbjct: 638 PLSPPKQRVV--NEHALNIMSEKLQRTNLKEQTP 669


>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
           L-lysine forming] |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 368

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +2

Query: 575 VVGMIWPPPLWMASVWSVTS*MLNRTPRKFSSAITPSL 688
           V G+  PPPL + S+  + + +   +   FS A+ PSL
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSL 342


>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
           Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 17/79 (21%), Positives = 34/79 (43%)
 Frame = -2

Query: 598 WWPNHSNN*KMLVCMSANCSAPSHGACISLEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 419
           W+P    +  +   +S   S+    + +  E    E  +  ++  LNR++ + F   Q+ 
Sbjct: 234 WFPKMETSLTLDELLSLFASSKELTSLVPNEKNPKEYILKLLFDHLNRKKTNNFNTHQIL 293

Query: 418 GTPMFIVKAYLPVNESFGF 362
                ++ A L V  +FGF
Sbjct: 294 SQSDVVLNALLSVATAFGF 312


>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
 Frame = +1

Query: 520 RLHETGLSS*QTCIQASSS-CWNDLATTSMDGISMECYIIDVESNTTQI 663
           ++ ET L +     ++S   CW++L+TTS +   +   II   + TT I
Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239


>SPAC5D6.09c |mug86||acetate transporter |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 304

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 18/60 (30%), Positives = 28/60 (46%)
 Frame = +1

Query: 145 VFLICFNYVVYNLSKY*VKSGRPSFNPFLFLVSCTTFRACSEVRTGLQGGPANGRIRTAG 324
           ++LIC+    + +    V+S    F+ F+ L  C    AC+ +RT   G P    IR  G
Sbjct: 191 LYLICWFIFTFLVLLCTVRSTLAFFSLFMSLDVCFLLLACAFLRTS-DGSPNVVLIRVGG 249


>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 935

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/31 (29%), Positives = 18/31 (58%)
 Frame = -2

Query: 532 SHGACISLEIQCPEVAVGGIYGVLNRRRGHV 440
           + GA +   I  P +A+   +G++N+R G +
Sbjct: 833 TQGALLMYTILVPFIALSAAFGIMNKRLGGI 863


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,860,411
Number of Sequences: 5004
Number of extensions: 62053
Number of successful extensions: 139
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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