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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV10412
         (849 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    55   1e-08
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    48   1e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    35   0.013
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    28   1.5  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    27   2.5  
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    27   4.4  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    27   4.4  
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo...    26   7.8  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 33/88 (37%), Positives = 49/88 (55%), Gaps = 6/88 (6%)
 Frame = +2

Query: 257 KDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDE 424
           +DG I  N+L     SLG+  +  EL +M+ E     +G I+FT+ LT+ A +M   +D 
Sbjct: 25  QDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-DTDN 83

Query: 425 DDVVINAFKTFDEEGK--IDSERLRHAL 502
           ++ V  AFK FD++G   I  E L H L
Sbjct: 84  EEEVREAFKVFDKDGNGYITVEELTHVL 111


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 29/83 (34%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
 Frame = +2

Query: 260 DGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVI 439
           DG IG+ D++    SL + ASE  ++ M    + PIN    LT   + +   S  +D ++
Sbjct: 62  DGNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND-LL 120

Query: 440 NAFKTFD--EEGKIDSERLRHAL 502
            AF TFD  + GKI    +R AL
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDAL 143



 Score = 30.3 bits (65), Expect = 0.36
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 171 KRTGSNVFSMFSQKQVAEFKEAFQLMD 251
           KR  S  F+  +  Q+ E KEAF L+D
Sbjct: 32  KRASSGAFAQLTSSQIQELKEAFALLD 58


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 35.1 bits (77), Expect = 0.013
 Identities = 21/86 (24%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
 Frame = +2

Query: 257 KDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVV 436
           KDG+I  + + +   SLG   ++ EL ++  E    I+  + ++  +N++     E++  
Sbjct: 22  KDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEE-Y 80

Query: 437 INAFKTFDEE--GKIDSERLRHALMT 508
           I AF+ FD++  G I++ +    + T
Sbjct: 81  IKAFRVFDKDNSGYIETAKFADYMKT 106


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 12/28 (42%), Positives = 18/28 (64%)
 Frame = +2

Query: 263 GIIGKNDLRATFDSLGRLASEKELDEMV 346
           G+IG  +LR    SLG   S +E+DE++
Sbjct: 92  GMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 25/71 (35%), Positives = 33/71 (46%)
 Frame = -1

Query: 444 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 265
           A I  S SS+PP      R  S V+     A   ++S+ FS    P ES  +  S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326

Query: 264 PSLCDPLAGMP 232
           PSL    A +P
Sbjct: 327 PSLESGKAELP 337


>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 231

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 15/36 (41%), Positives = 18/36 (50%)
 Frame = -1

Query: 339 SSSSFSDASLPRESKVARKSFLPMMPSLCDPLAGMP 232
           SSSS S +S P   + +RK   P MP L   L   P
Sbjct: 3   SSSSSSGSSSPASKRSSRKRQTPPMPELPSFLLAFP 38


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
 Frame = -1

Query: 420 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 259
           S P   R+A+ +K S VKL + P+A   + SS+ S ASL  ES   R S  P  PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595


>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
           Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 457

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
 Frame = -1

Query: 354 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 259
           +SP++   +++  + P++S V+RKSF  P  P+
Sbjct: 35  SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,845
Number of Sequences: 5004
Number of extensions: 45348
Number of successful extensions: 159
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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