Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0027
         (691 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch...    59   7e-10
SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe...    29   0.84 
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ...    28   1.1  
SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |...    28   1.5  
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...    27   1.9  
SPAC26H5.03 |||WD repeat protein Cac2|Schizosaccharomyces pombe|...    27   1.9  
SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p...    27   1.9  

>SPAC25G10.08 |||translation initiation factor eIF3b
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 725

 Score = 58.8 bits (136), Expect = 7e-10
 Identities = 36/97 (37%), Positives = 55/97 (56%), Gaps = 13/97 (13%)
 Frame = +2

Query: 2   ERKPFNHLFWSPSGQFIVLANLGL-TGGALEFLDTNDFT-------IMNV-----ADHYQ 142
           ++K  N LF +P G+F+V A LG  T   LEF D +  T       + NV     A+H+ 
Sbjct: 512 DKKTCNSLFMAPKGRFMVAATLGSSTQYDLEFYDLDFDTEKKEPDALANVQQIGSAEHFG 571

Query: 143 MSGIEWDPTGRYVVTGVSSXKCKMDCGYYIWSFQGKI 253
           M+ +EWDP+GRYV T  +  + K++ GY +  F+G +
Sbjct: 572 MTELEWDPSGRYVTTSSTIWRHKLENGYRLCDFRGTL 608



 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 36/118 (30%), Positives = 53/118 (44%), Gaps = 1/118 (0%)
 Frame = +1

Query: 148 WDRMGSNRTLCSD-WCVFXQM*NGLRLLHLVIPGQDTRRVMKEGFAQFHWRPRPPTLLSX 324
           WD  G   T  S  W    ++ NG RL      G   R  M   F QF WRPRPP+ L+ 
Sbjct: 577 WDPSGRYVTTSSTIWR--HKLENGYRLCDF--RGTLLREEMIGEFKQFIWRPRPPSPLTK 632

Query: 325 XXXXXXXXXXXXYYSQFESKDRMRSSKASKELVAKRTEQMKKFTEYRESKIQEWNEQK 498
                       Y   F+ +D    S A++EL A+R + + ++ +YR+  I    E++
Sbjct: 633 EDMKKIRKKLKDYNRLFDEEDIAEQSSANRELAARRRQLISEWQKYRDEVIARVAEER 690


>SPAC140.04 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 295

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 4/45 (8%)
 Frame = +1

Query: 361 YYSQFESKDRMRSSKASKELVAKRTEQMKKFTE----YRESKIQE 483
           Y   F+ K+R     + KE+ AK T+Q +K TE     R+ K++E
Sbjct: 226 YQFSFDEKEREEQLLSLKEIHAKVTQQQRKNTEDVLTLRDKKLEE 270


>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 509

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 16/64 (25%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
 Frame = +2

Query: 5   RKPFNHLFWSPSGQFIVLANLGLTGGALEFLD--TNDFTIMNVADHYQMSGIEWDPTGRY 178
           R   N + W+P G+ ++    G + G     +  T +F ++N +  Y +   EW   GR+
Sbjct: 81  RHVINVVRWTPDGRRLLT---GSSTGEFTLWNGLTFNFELINQSHDYAVRCAEWSTDGRW 137

Query: 179 VVTG 190
           +++G
Sbjct: 138 LISG 141


>SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 760

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 3/56 (5%)
 Frame = +2

Query: 29  WSPSGQFIVLANLGLTGG-ALEFLDTNDFT-IMNVADHYQ-MSGIEWDPTGRYVVT 187
           W+P+G  +V    G TGG  L   D   +  + +++ H + + G+ WDP G++ ++
Sbjct: 334 WNPNGNCVVC--WGRTGGWRLWKQDAGQWLQLPSISGHTKSVKGVAWDPEGKFYLS 387


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 14/47 (29%), Positives = 23/47 (48%)
 Frame = +1

Query: 400 SKASKELVAKRTEQMKKFTEYRESKIQEWNEQKPRRPNLEIM*TQTV 540
           S A KE+V +  E+         S+I+ W E KP+    ++   QT+
Sbjct: 207 SSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTI 253


>SPAC26H5.03 |||WD repeat protein Cac2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 512

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
 Frame = +2

Query: 23  LFWSPSGQFIVLANLGLTGGALEFLDTNDFTIMNVA-DH-YQMSGIEWDPTGRYVVT 187
           L WS    F++    G    +L   D +   ++    DH + + G+ WDP  +Y+V+
Sbjct: 134 LCWSVDSNFLIA---GAMDNSLRLYDAHTGQLLTQKFDHSHYVQGVCWDPLNQYIVS 187


>SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 932

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 16/56 (28%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
 Frame = +2

Query: 23  LFWSPSGQFIVLANLGLTGGALEFLDTNDFTIMNVADHYQ-MSGIEWDPTGRYVVT 187
           L WS   Q +V  ++GL    + +  T    +  +  H   + GI +DP G+Y  T
Sbjct: 140 LCWSYDSQLVV--SVGLDSSIIVWNGTTFERLKRIEAHQSHVKGITFDPAGKYFAT 193


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,836
Number of Sequences: 5004
Number of extensions: 48504
Number of successful extensions: 164
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)