Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0018.Seq
         (399 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ...    28   0.61 
SPAC1F12.08 |||cytidylyltransferase |Schizosaccharomyces pombe|c...    27   1.1  
SPAC1805.02c |||electron transfer flavoprotein beta subunit |Sch...    26   2.5  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    25   4.3  
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom...    25   4.3  
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch...    25   4.3  
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c...    25   5.7  
SPAC24B11.09 |||conserved eukaryotic protein|Schizosaccharomyces...    25   5.7  
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ...    24   7.6  
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb...    24   7.6  
SPAC144.04c |spe1||ornithine decarboxylase Spe1|Schizosaccharomy...    24   7.6  
SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p...    24   7.6  
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    24   10.0 
SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe...    24   10.0 

>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1877

 Score = 27.9 bits (59), Expect = 0.61
 Identities = 14/46 (30%), Positives = 24/46 (52%)
 Frame = +2

Query: 23   IYFEGAKTFLSKGIFIFNVVTYLFRHHYASRQGIGSDDKRHGAHID 160
            I F+ A++   + +  ++V+TYL +        I  DD+ H  HID
Sbjct: 1704 IAFQKARSNFVQSMAAYSVITYLLQFKDRHNGNIMIDDQGHILHID 1749


>SPAC1F12.08 |||cytidylyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 329

 Score = 27.1 bits (57), Expect = 1.1
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = -3

Query: 271 VLHQVQTVHLFHYSGTCAMGSVVDSKMKVYGIDNLR 164
           +L  V T+ LF Y     +GS+ DS   VY  +N +
Sbjct: 78  LLSVVPTIVLFEYWSNIQLGSITDSWNSVYYSENAK 113


>SPAC1805.02c |||electron transfer flavoprotein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 254

 Score = 25.8 bits (54), Expect = 2.5
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = +1

Query: 244 GGRSGLDVTLPIILTTDVHL 303
           GG   L  TLP I+TTD+ L
Sbjct: 167 GGEETLSSTLPAIITTDLRL 186


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 15/48 (31%), Positives = 28/48 (58%)
 Frame = -2

Query: 365 IPLISKISKRK*LIPAWKNAVRWTSVVRIIGSVTSSPDRPPIPLLRYM 222
           +P I +ISK   L  +  ++  +T++  II +  S P+R P+ + +YM
Sbjct: 380 LPCIFEISKG--LDSSIFSSKVFTAIFPIISAANSYPERVPLCIFQYM 425


>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
           hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 429

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
 Frame = +1

Query: 235 NGI--GGRSGLDVTLPIILTTDVHLTAFFQAGISYF 336
           NGI  GG SGL + +P  +  +  + A  + GI YF
Sbjct: 160 NGITMGGGSGLAMHVPFRIACEDTMFAMPETGIGYF 195


>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
            Mok12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2352

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 11/19 (57%), Positives = 13/19 (68%)
 Frame = +3

Query: 3    FFFYRILFILKAPRHFSQR 59
            FFF+ I FIL A  HF Q+
Sbjct: 2001 FFFFGISFILVAITHFFQK 2019


>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 796

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 10/50 (20%), Positives = 26/50 (52%)
 Frame = +3

Query: 12  YRILFILKAPRHFSQREYSFLMSSLIFSAIIMPAAKVLALTIKGMVLTST 161
           Y +++ ++ P+H +   +S +   +IF  +IM    +  ++++     ST
Sbjct: 672 YELIYQMEHPQHSTGELWSTIFLRMIFGCVIMQLTMMGLMSLRKAYWLST 721


>SPAC24B11.09 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 118

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 11/33 (33%), Positives = 16/33 (48%)
 Frame = -2

Query: 317 WKNAVRWTSVVRIIGSVTSSPDRPPIPLLRYMC 219
           W  A++WT V+  IG    SP+   I     +C
Sbjct: 22  WAPAMKWTLVLSGIGDYARSPEYLSIRQYAALC 54


>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 2344

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 7/18 (38%), Positives = 13/18 (72%)
 Frame = -1

Query: 315  EECGEMDVSSEDYWKCYI 262
            E CGE++++  + WK Y+
Sbjct: 1691 EYCGELNINDPNTWKEYV 1708


>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 300

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 11/28 (39%), Positives = 14/28 (50%)
 Frame = +2

Query: 41  KTFLSKGIFIFNVVTYLFRHHYASRQGI 124
           K FLSK     N +T +   H A  +GI
Sbjct: 80  KVFLSKTQSSLNPITLILNRHEAGNEGI 107


>SPAC144.04c |spe1||ornithine decarboxylase Spe1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 12/52 (23%), Positives = 25/52 (48%)
 Frame = -3

Query: 217 MGSVVDSKMKVYGIDNLRVVDVSTMPFIVRANTLAAGIMMAEKISDDIKNEY 62
           +  ++ S +  Y   ++RV+      F+  + TLA  ++   K+ D+ K  Y
Sbjct: 275 VADLIRSALDTYFDPSIRVISEPGRFFVSSSFTLAVNVIAKRKLDDEEKVMY 326


>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 579

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 7/22 (31%), Positives = 14/22 (63%)
 Frame = -1

Query: 300 MDVSSEDYWKCYIKSRPSTYSI 235
           +DV ++++WK ++   PS   I
Sbjct: 403 LDVETQNFWKLFMSGNPSQEEI 424


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 19/52 (36%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
 Frame = +3

Query: 21  LFILKAPRHFSQRE-YSFLMSSLIFSAIIMPAAKVLALTIKGMVLTSTTRRL 173
           LF+L  P HF Q E    L SS      +   A +L L     VLT  T  L
Sbjct: 84  LFLLGEPTHFGQIECLKLLSSSRFMDKRLGYLAAMLLLDENQEVLTLLTNSL 135


>SPAC323.07c |||MatE family transporter|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 533

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +3

Query: 129 LTIKGMVLTSTTRRLSIPYTFILLSTT 209
           L  + ++LTST+    IP+ F + S+T
Sbjct: 349 LASQSILLTSTSLLFQIPFAFAVASST 375


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,756,494
Number of Sequences: 5004
Number of extensions: 36938
Number of successful extensions: 92
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)