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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00004
         (579 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...   167   1e-42
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...   167   1e-42
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...   111   7e-26
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...   111   7e-26
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...   111   7e-26
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro...    73   2e-14
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc...    38   8e-04
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    31   0.16 
SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc...    27   2.6  
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha...    25   6.1  
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr...    25   6.1  

>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  167 bits (406), Expect = 1e-42
 Identities = 86/150 (57%), Positives = 106/150 (70%), Gaps = 2/150 (1%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 182 LQYPEGIHPPPGVEASWWR*ETQEENYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLR 361
           +Q    +H    +     +   +++ Y+TP           LAVL++YKV+++G + RLR
Sbjct: 61  IQKESTLHLVLRLRGGGKK--RKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLR 118

Query: 362 RECTGEQCGAGVFMAVMEDRHYCGKCHSTM 451
           REC    CGA  FMA  +DR YCG+CH T+
Sbjct: 119 REC--PNCGASTFMANHKDRLYCGRCHLTL 146


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  167 bits (406), Expect = 1e-42
 Identities = 86/150 (57%), Positives = 106/150 (70%), Gaps = 2/150 (1%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 182 LQYPEGIHPPPGVEASWWR*ETQEENYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLR 361
           +Q    +H    +     +   +++ Y+TP           LAVL++YKV+++G + RLR
Sbjct: 61  IQKESTLHLVLRLRGGGKK--RKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLR 118

Query: 362 RECTGEQCGAGVFMAVMEDRHYCGKCHSTM 451
           REC    CGA  FMA  +DR YCG+CH T+
Sbjct: 119 REC--PNCGASTFMANHKDRLYCGRCHLTL 146


>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 61  IQKESTLH 68



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 57  SDYNIQKESTLHLV 70


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 61  IQKESTLH 68



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 57  SDYNIQKESTLHLV 70


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 61  IQKESTLH 68



 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 77  MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 137 IQKESTLH 144



 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 213 IQKESTLH 220



 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 289 IQKESTLH 296



 Score =  111 bits (267), Expect = 7e-26
 Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL  Y 
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364

Query: 182 LQYPEGIH 205
           +Q    +H
Sbjct: 365 IQKESTLH 372



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 57  SDYNIQKESTLHLV 70



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 133 SDYNIQKESTLHLV 146



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 209 SDYNIQKESTLHLV 222



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 285 SDYNIQKESTLHLV 298



 Score = 31.5 bits (68), Expect = 0.092
 Identities = 13/14 (92%), Positives = 14/14 (100%)
 Frame = +3

Query: 177 TDYNIQKESTLHLV 218
           +DYNIQKESTLHLV
Sbjct: 361 SDYNIQKESTLHLV 374


>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
           protein modifier Ned8|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 78

 Score = 73.3 bits (172), Expect = 2e-14
 Identities = 30/54 (55%), Positives = 44/54 (81%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGR 169
           M I VKTLTGK I L+++P+D +  +K ++++KEGIPP+QQRLI+AGKQ+ D +
Sbjct: 1   MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDK 54


>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
           Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 38.3 bits (85), Expect = 8e-04
 Identities = 16/43 (37%), Positives = 27/43 (62%)
 Frame = +2

Query: 35  GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLED 163
           GK   LE+EP++T   +K ++     +PP +Q++I  G QL+D
Sbjct: 10  GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKD 52


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 30.7 bits (66), Expect = 0.16
 Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
 Frame = +2

Query: 8   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
           + + +K    +   + V+   ++  +K  I     I   +QRLI+AG+ L+D  +L  Y 
Sbjct: 4   ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63

Query: 182 LQYPEGIH 205
           +Q    IH
Sbjct: 64  IQDGHSIH 71


>SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
 Frame = +2

Query: 311 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 469
           V+R Y ++ +N +     R C  E      F  V++D H   KC + ++    D
Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277


>SPCC1020.08 |||wybutosine biosynthesis protein
           Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 688

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 9/30 (30%), Positives = 21/30 (70%), Gaps = 1/30 (3%)
 Frame = -2

Query: 185 VVRRGYVHLPAA-FQQRSTSVDWEGFPLCL 99
           V +RG +  P++  ++++ ++DW+  PLC+
Sbjct: 64  VGKRGKIGSPSSSIRKQNDTIDWKNSPLCV 93


>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
           heterochromatin assembly Hrr1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1015

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = -3

Query: 442 VTLPAVVTVLHHRHEHSGAALLAC 371
           VTL A+ T+L + H H    L+AC
Sbjct: 402 VTLKAIETLLENTHSHVLPILVAC 425


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,288,671
Number of Sequences: 5004
Number of extensions: 44691
Number of successful extensions: 133
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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