Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00003
         (711 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces...    94   2e-20
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb...    28   1.1  
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces...    27   2.6  
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc...    27   3.5  
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth...    27   3.5  

>SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 208

 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 41/74 (55%), Positives = 51/74 (68%)
 Frame = +2

Query: 35  IPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPTVRYHT 214
           +PN HFHKDWQR+VKTWFNQP R+ RR+Q R               +RP V+ PT+RY+ 
Sbjct: 9   LPNAHFHKDWQRYVKTWFNQPGRKLRRRQAR-QTKAAKIAPRPVEAIRPAVKPPTIRYNM 67

Query: 215 KVRAGRGFTLREIR 256
           KVRAGRGFTL E++
Sbjct: 68  KVRAGRGFTLEELK 81



 Score = 59.7 bits (138), Expect = 4e-10
 Identities = 37/78 (47%), Positives = 49/78 (62%), Gaps = 1/78 (1%)
 Frame = +3

Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
           A TIGI VD RRRN+S ESLQ NV+RIK Y A LI+FP K  +  KG+A +      T +
Sbjct: 90  ASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFPRKAGQPKKGDATDVSGAEQTDV 149

Query: 456 RGPLMPVQQPAPKSVARP 509
              ++P+ Q A +  A+P
Sbjct: 150 -AAVLPITQEAVEE-AKP 165



 Score = 33.9 bits (74), Expect = 0.023
 Identities = 17/41 (41%), Positives = 22/41 (53%)
 Frame = +2

Query: 503 KTITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDAAENPD 625
           K ITE+ KNF A+  L   R+ A+  G RA   K  AE  +
Sbjct: 164 KPITEEAKNFNAFSTLSNERAYARYAGARAAFQKKRAEEAE 204


>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1283

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 4/44 (9%)
 Frame = -3

Query: 247  TKSESSTGAYFSM----VPNSWASHYRT*RPSCRTWSYGLSFLY 128
            T   +STG+Y  M    +   W S   T    C TWSY  S+ Y
Sbjct: 1215 TVQGTSTGSYICMPHFQIQYDWCSAGVTDMSECNTWSYQKSYDY 1258


>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 815

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 12/39 (30%), Positives = 20/39 (51%)
 Frame = -1

Query: 303 LQQFQSFGQILGSICGLISRRVNPLPARTLVWYRTVGHR 187
           LQ    F  ++ ++C L+  + NP+  R  V Y TV  +
Sbjct: 292 LQSQGDFAFVVRTLCDLLKLKPNPISGRDPVLYATVSQQ 330


>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 11/31 (35%), Positives = 20/31 (64%)
 Frame = +2

Query: 527 NFKAYQYLRGARSIAKLVGIRAKRLKDAAEN 619
           N ++ ++LR A S A++VG   +R++   EN
Sbjct: 843 NNRSEEFLRNAASQAEIVGANKERIQKTVEN 873


>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
           Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1323

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 14/27 (51%), Positives = 16/27 (59%)
 Frame = +2

Query: 227 GRGFTLREIRPQIEPSICPNDWNCCRS 307
           G  F LR+I P IEPS+ P    CC S
Sbjct: 527 GARFELRDI-PCIEPSMSPMQIWCCES 552


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,697,038
Number of Sequences: 5004
Number of extensions: 54172
Number of successful extensions: 160
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)