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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_A12
         (525 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|...   234   7e-63
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz...   234   7e-63
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...   149   3e-37
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...   149   3e-37
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...   149   3e-37
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro...    89   5e-19
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    45   8e-06
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb...    44   2e-05
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc...    41   1e-04
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy...    29   0.56 
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha...    25   5.2  
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha...    25   5.2  
SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomy...    25   9.1  

>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
           Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  234 bits (572), Expect = 7e-63
 Identities = 109/123 (88%), Positives = 114/123 (92%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 221 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRATNCRKTKCGHTNN 400
           IQKESTLHLVLRL GG IEPSL+ LA KYNC K ICRKCYARL PRATNCRK KCGHTN 
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQ 120

Query: 401 LRP 409
           LRP
Sbjct: 121 LRP 123


>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
           Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 128

 Score =  234 bits (572), Expect = 7e-63
 Identities = 109/123 (88%), Positives = 114/123 (92%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 221 IQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRATNCRKTKCGHTNN 400
           IQKESTLHLVLRL GG IEPSL+ LA KYNC K ICRKCYARL PRATNCRK KCGHTN 
Sbjct: 61  IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGHTNQ 120

Query: 401 LRP 409
           LRP
Sbjct: 121 LRP 123


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 61  IQKESTLHLVLRLRGG 76


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 61  IQKESTLHLVLRLRGG 76



 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 77  MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 137 IQKESTLHLVLRLRGG 152



 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 213 IQKESTLHLVLRLRGG 228



 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 289 IQKESTLHLVLRLRGG 304



 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 365 IQKESTLHLVLRLRGG 380


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score =  149 bits (360), Expect = 3e-37
 Identities = 71/76 (93%), Positives = 75/76 (98%)
 Frame = +2

Query: 41  LQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 220
           +QIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 221 IQKESTLHLVLRLXGG 268
           IQKESTLHLVLRL GG
Sbjct: 61  IQKESTLHLVLRLRGG 76


>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
           protein modifier Ned8|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 78

 Score = 88.6 bits (210), Expect = 5e-19
 Identities = 38/75 (50%), Positives = 55/75 (73%)
 Frame = +2

Query: 47  IFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 226
           I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++
Sbjct: 3   IKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLE 62

Query: 227 KESTLHLVLRLXGGT 271
             S LHLVL L GG+
Sbjct: 63  GGSVLHLVLALRGGS 77


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 44.8 bits (101), Expect = 8e-06
 Identities = 22/75 (29%), Positives = 40/75 (53%)
 Frame = +2

Query: 35  TQLQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD 214
           T + + +K    +   + V++  ++  +K  I     I  ++QRLI+AG+ L+D  +L  
Sbjct: 2   TNISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKT 61

Query: 215 YNIQKESTLHLVLRL 259
           Y IQ   ++HLV  L
Sbjct: 62  YKIQDGHSIHLVKTL 76


>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 368

 Score = 43.6 bits (98), Expect = 2e-05
 Identities = 18/54 (33%), Positives = 35/54 (64%)
 Frame = +2

Query: 71  KTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 232
           K +  +V A   I  +K KIQ ++    ++Q+LI++G+ L D +T+ +YNI+++
Sbjct: 12  KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65


>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
           Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 19/58 (32%), Positives = 33/58 (56%)
 Frame = +2

Query: 68  GKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL 241
           GK   LE+E ++T   +K ++     +PP++Q++I  G QL+D   L    I+  +TL
Sbjct: 10  GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67


>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 603

 Score = 28.7 bits (61), Expect = 0.56
 Identities = 21/81 (25%), Positives = 38/81 (46%), Gaps = 2/81 (2%)
 Frame = +2

Query: 35  TQLQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD 214
           ++ +I V T+  K    +V  + T+  +K  I      P D+ +LI AG+ L +   L +
Sbjct: 2   SEYRIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEE 61

Query: 215 --YNIQKESTLHLVLRLXGGT 271
             ++     T HLV+ +   T
Sbjct: 62  ILHDATDLVTFHLVIAVFNST 82


>SPBC776.09 |ste13||ATP-dependent RNA helicase
           Ste13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 13/116 (11%)
 Frame = +2

Query: 56  KTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA------GKQLE-------D 196
           K  TGKT    + + + ++  K+KIQ    +P  +  L  +      GK +         
Sbjct: 89  KNGTGKTAAFVIPSLEKVDTKKSKIQTLILVPTRELALQTSQVCKTLGKHMNVKVMVTTG 148

Query: 197 GRTLSDYNIQKESTLHLVLRLXGGTIEPSLRILAMKYNCXKMICRKCYARLHPRAT 364
           G TL D  I+   T+H+V+   G  ++ + + +A    C   +  +    L P  T
Sbjct: 149 GTTLRDDIIRLNDTVHIVVGTPGRVLDLAGKGVADFSECTTFVMDEADKLLSPEFT 204


>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = -3

Query: 115 IFDSVGSFHLQCNGLPRKGFDEYLQ 41
           IF  VG+F   C+ L ++GF E L+
Sbjct: 43  IFPGVGNFGFVCDSLAKQGFLEPLR 67


>SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 355

 Score = 24.6 bits (51), Expect = 9.1
 Identities = 18/64 (28%), Positives = 25/64 (39%), Gaps = 3/64 (4%)
 Frame = +2

Query: 62  LTGKTITLEVEASDTIENVKAKIQDKEGIP---PDQQRLIFAGKQLEDGRTLSDYNIQKE 232
           L G   T EV    T+   +    + +G P   P  +  I A     +G TL+ YN    
Sbjct: 94  LKGPERTSEVSLPQTVNLSEISNNNDKGQPTNTPPVRSTIVAPSLYSEGSTLNHYNNGSH 153

Query: 233 STLH 244
             LH
Sbjct: 154 QVLH 157


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,091,950
Number of Sequences: 5004
Number of extensions: 42146
Number of successful extensions: 115
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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