Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20016
         (648 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz...   107   2e-24
SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch...    34   0.020
SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual    27   1.8  
SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe...    27   3.1  
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch...    26   4.1  
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2...    26   4.1  
SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei...    26   4.1  
SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces po...    26   4.1  
SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces po...    26   5.4  
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    25   7.1  
SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual        25   9.4  
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo...    25   9.4  

>SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 440

 Score =  107 bits (256), Expect = 2e-24
 Identities = 53/85 (62%), Positives = 64/85 (75%), Gaps = 1/85 (1%)
 Frame = +1

Query: 1   ILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFAKFN-APAPDETYGRGRDWNVDLI 177
           +LSG+LSV GKKVLHIDRN YYG +SAS+  L +L+A F       E+ GR RDW VDL+
Sbjct: 18  VLSGLLSVDGKKVLHIDRNDYYGADSASLN-LTQLYALFRPGEQRPESLGRDRDWCVDLV 76

Query: 178 PKFLMANGLLVKLLIHTGVTRYLEF 252
           PKFLMANG L  +LI+T VTRY+EF
Sbjct: 77  PKFLMANGDLTNILIYTDVTRYIEF 101



 Score = 97.1 bits (231), Expect = 2e-21
 Identities = 43/85 (50%), Positives = 62/85 (72%)
 Frame = +3

Query: 258 IEGSYVYKGGKISKVPVDQKEALASDLMGMFEKRRFRNFLIYVQDFQEEDAKTWKDFDPS 437
           I GSYVY+ G+I+KVP ++ EAL S LM +FEKRR + FL +V +++E+D  T+KD +  
Sbjct: 104 IAGSYVYRDGRIAKVPGNEMEALKSPLMSLFEKRRAKKFLEWVNNYREDDPSTYKDINID 163

Query: 438 TANMQSLYDKFGLDRNTQDFTGHAL 512
             +M+S++ KFGL   TQDF GHA+
Sbjct: 164 RDSMESVFKKFGLQSGTQDFIGHAM 188



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 21/34 (61%), Positives = 28/34 (82%)
 Frame = +2

Query: 509 LALYLDDNYLQQPAIQTIRRIKLYSDSLAKYGKS 610
           +ALYLDD YL++PA +T  RI LY+ S+AK+GKS
Sbjct: 188 MALYLDDAYLKKPARETRERILLYASSIAKFGKS 221


>SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score = 33.9 bits (74), Expect = 0.020
 Identities = 15/30 (50%), Positives = 20/30 (66%)
 Frame = +1

Query: 1   ILSGMLSVSGKKVLHIDRNKYYGGESASIT 90
           ILS  LS + ++VLHID N +YG    S+T
Sbjct: 20  ILSAALSWANQRVLHIDENSFYGEIDGSLT 49


>SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 684

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 15/57 (26%), Positives = 27/57 (47%)
 Frame = +1

Query: 28  GKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETYGRGRDWNVDLIPKFLMAN 198
           GKK +  +RN Y   ++    PL     K + P+  ++  + +D N D   + L+ N
Sbjct: 2   GKKSVSFNRNNYKKRKNERTEPLPRRIFKNDKPSKFKSKRKEKDKNSDAYDEMLLNN 58


>SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 466

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 12/31 (38%), Positives = 15/31 (48%)
 Frame = +1

Query: 94  LEELFAKFNAPAPDETYGRGRDWNVDLIPKF 186
           L  L  K  A AP +TY   + W    +PKF
Sbjct: 32  LRSLIEKEEAAAPPKTYEDFKFWKTQPVPKF 62


>SPBC1289.02c |uap2||U2 snRNP-associated protein
           Uap2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 367

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +3

Query: 369 NFLIYVQDFQEEDAKTWKDFDPSTANM 449
           N LIY+ + Q     TWKD+DP++  M
Sbjct: 22  NELIYIDEEQ-----TWKDYDPNSLKM 43


>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
           Mei2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 750

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
 Frame = +2

Query: 221 FTRASPGT-WSLIHRRKLCLQRWKNLQSTCR---SERSSCV 331
           F +A  GT W++ H  K+C   + N+Q   R     R+SCV
Sbjct: 656 FGKARVGTQWNVFHSEKICDISYANIQGKDRLIEKFRNSCV 696


>SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 945

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 11/21 (52%), Positives = 17/21 (80%), Gaps = 1/21 (4%)
 Frame = -1

Query: 273 HNF-LRWIKLQVPGDARVNKQ 214
           HNF +  I++Q+PGDA+ NK+
Sbjct: 292 HNFSVPEIRIQLPGDAKENKE 312


>SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 230

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 10/24 (41%), Positives = 18/24 (75%)
 Frame = +2

Query: 17  CRFLGRRFCTSIAISTTVVNLRQL 88
           CR L R+F  ++ +ST++V ++QL
Sbjct: 116 CRILTRKFRLAVYLSTSLVYIKQL 139


>SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 166

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = +1

Query: 124 PAPDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGV 234
           P P +  G  + + V     F++ANG L+K LIH  +
Sbjct: 14  PRPAQALGINQPF-VSAYLSFVLANGFLLKWLIHYSI 49


>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 16/50 (32%), Positives = 27/50 (54%)
 Frame = -2

Query: 350 KHAHKIRRKSFFLIDRYFGDFSTFVNITSFDGLNSKYRVTPV*ISNLTSN 201
           K +HK+ ++S FL D +  DF+ F+ I   D + ++    PV  S  T +
Sbjct: 378 KFSHKMLKRSQFLNDHFDADFN-FIQI-DHDPITNQETRKPVKFSKPTKD 425


>SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 583

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 12/35 (34%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
 Frame = +1

Query: 157 DWNVDLIPKFLMANGLLVKL-LIHTGVTRYLEFNP 258
           D  +D +  + M NG+L    ++H  + RYL+ NP
Sbjct: 68  DATIDQLQNY-MENGILTSTDIVHCYLDRYLQVNP 101


>SPAC3F10.16c |||GTP binding protein,
           HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 616

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 11/32 (34%), Positives = 16/32 (50%)
 Frame = +1

Query: 40  LHIDRNKYYGGESASITPLEELFAKFNAPAPD 135
           L  D N  +    A++  LE +F KF +  PD
Sbjct: 266 LQADENDVHSSRIATLKVLEGIFEKFASTLPD 297


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,835,850
Number of Sequences: 5004
Number of extensions: 59536
Number of successful extensions: 186
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)