Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20010
(420 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 80 1e-16
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 66 2e-12
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 36 0.002
SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 27 1.2
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom... 26 2.1
SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr 1||... 26 2.7
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch... 25 3.6
SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp... 25 3.6
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 25 6.3
SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 24 8.4
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 24 8.4
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 24 8.4
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 80.2 bits (189), Expect = 1e-16
Identities = 35/76 (46%), Positives = 52/76 (68%)
Frame = +3
Query: 30 MAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEK 209
MA ++ G +A + +LR+Q++ ++S F L I+QVGGREDSNVY+RMK +AA +
Sbjct: 1 MALLLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANE 60
Query: 210 IGIAAEHIRLPRDITK 257
GI+ EH+ P DIT+
Sbjct: 61 AGISCEHVNFPEDITE 76
Score = 52.8 bits (121), Expect = 2e-08
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +2
Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
+LL I NE P+VHGIIVQ+PL + I+ +T+AV+P+KDVDG N
Sbjct: 78 DLLLAIKGFNEDPTVHGIIVQLPLPAH--INEQIITEAVAPEKDVDGFCETN 127
>SPBC2G2.08 |ade9||C-1-
tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 969
Score = 66.5 bits (155), Expect = 2e-12
Identities = 33/73 (45%), Positives = 48/73 (65%)
Frame = +3
Query: 33 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 212
A I++G +A ++ + ++V L++K F+P LAIVQVG REDSNVY+RMK KAA +
Sbjct: 33 ANILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLV 92
Query: 213 GIAAEHIRLPRDI 251
GI ++ P I
Sbjct: 93 GIDFKYCPFPETI 105
Score = 46.4 bits (105), Expect = 2e-06
Identities = 20/51 (39%), Positives = 33/51 (64%)
Frame = +2
Query: 263 LLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
LL ++ LN+ +VHG++VQ+PL ++ VT++++P KDVDG N
Sbjct: 110 LLHELKKLNDDHTVHGVLVQLPLPKH--LNERTVTESITPPKDVDGFGAFN 158
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 36.3 bits (80), Expect = 0.002
Identities = 19/43 (44%), Positives = 27/43 (62%)
Frame = +2
Query: 275 ITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGL 403
I N PSV+GI++ P+ +D D + + VSPDKDV+GL
Sbjct: 85 IVEANNDPSVNGIMIYFPVFNDGQ-DQY-LQQVVSPDKDVEGL 125
>SPAC14C4.08 |mug5||meiotically upregulated gene
Mug5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 179
Score = 27.1 bits (57), Expect = 1.2
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +3
Query: 117 SGFEPRLAIVQVGGRE-DSNVYIRMKLKAAEKI 212
SGFE R+ I++ E DSN+YIR+K + K+
Sbjct: 11 SGFENRIKILEDLMNEFDSNLYIRLKRECDFKL 43
>SPBC1A4.07c |||U3 snoRNP-associated protein
Sof1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 436
Score = 26.2 bits (55), Expect = 2.1
Identities = 13/51 (25%), Positives = 24/51 (47%)
Frame = +3
Query: 81 LRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHI 233
L + +T+LR+ + P A V G ED N+Y+ + + +H+
Sbjct: 223 LTKLITKLRTNSISWNPMEAFNFVAGSEDHNLYMYDMRNLKRALHVYKDHV 273
>SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 247
Score = 25.8 bits (54), Expect = 2.7
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +3
Query: 54 EVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRM--KLKAAEK 209
E+ S++ D+RQ++ +L + S E I + RE IRM +L++AE+
Sbjct: 31 EIESSLK-DVRQELQKLNEEQSRLEKNAQIPEYRVRESEAFLIRMQRRLESAEE 83
>SPBC1289.02c |uap2||U2 snRNP-associated protein
Uap2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 3.6
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +2
Query: 257 IELLAKITSLNESPSVHGIIVQMPLD-SDHAIDAHRVTDAVSPDKDVDGLNTI 412
I +L I +L E +++ + D ++ A RVT+ V DK+ DG+ T+
Sbjct: 250 IVVLKHIFTLEELDKTPELLIDLKDDITEEAEKCGRVTNVVLYDKEPDGVVTV 302
>SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog,
Rhp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 25.4 bits (53), Expect = 3.6
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +1
Query: 214 VSPPNISAYQGTSLNRAVSKNHESERISFRSRH 312
V+P NI +QG L+ + K+ + E SF H
Sbjct: 478 VAPVNIRRFQGRDLSFSTLKDMKFEETSFLPSH 510
>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 745
Score = 24.6 bits (51), Expect = 6.3
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 317 VQMPLDSDHAIDAHRVTDAVSPDKD 391
++ P+D D+A H +T SPD D
Sbjct: 185 LEAPIDFDYASVHHNLTALSSPDTD 209
>SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor
Crm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1078
Score = 24.2 bits (50), Expect = 8.4
Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 3/81 (3%)
Frame = +3
Query: 24 AKMAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLA---IVQVGGREDSNVYIRMKL 194
A+ AQ++ + + N L+ V S SGF P++A + +G + + I +
Sbjct: 674 AQNAQVLGDPQTVKILANVLKTNVAACTSIGSGFYPQIAKNYVDMLGLYKAVSGLISEVV 733
Query: 195 KAAEKIGIAAEHIRLPRDITK 257
A I H+R R I K
Sbjct: 734 AAQGNIATKTPHVRGLRTIKK 754
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 24.2 bits (50), Expect = 8.4
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +1
Query: 1 RPTRQFSGLRWHKLYLGLK 57
RPTR+ + W KL GL+
Sbjct: 985 RPTRRLKQMHWEKLNSGLE 1003
>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 24.2 bits (50), Expect = 8.4
Identities = 13/52 (25%), Positives = 25/52 (48%)
Frame = +2
Query: 263 LLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTINE 418
LL ++ P + +D+ +A+D T+ ++ D+DVD L + E
Sbjct: 542 LLLRLRQACNHPQSLNFQFEQDVDAFNALDGAANTNKLASDQDVDDLANLLE 593
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,538,494
Number of Sequences: 5004
Number of extensions: 26298
Number of successful extensions: 66
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 61
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 64
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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