Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV30036
         (543 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    60   2e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    40   3e-04
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    36   0.004
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.016
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p...    29   0.34 
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    29   0.34 
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    27   1.8  
SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo...    26   3.1  
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    26   4.1  
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    26   4.1  
SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF...    25   7.2  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    25   7.2  
SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual    25   7.2  
SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces po...    25   9.5  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 28/85 (32%), Positives = 50/85 (58%)
 Frame = -2

Query: 491 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 312
           EF+T+ A+ + + D E   +E+REAF+++DK+GNGYI    L  +               
Sbjct: 69  EFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125

Query: 311 LIQEIDTDGSGTVDFDEFMEMMTGE 237
           +I+E DTDG G ++++EF  +++ +
Sbjct: 126 MIREADTDGDGVINYEEFSRVISSK 150



 Score = 50.0 bits (114), Expect = 2e-07
 Identities = 23/62 (37%), Positives = 35/62 (56%)
 Frame = -2

Query: 431 ELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFME 252
           E REAF L+D++ +G I ++ L  +               +I E+D DG+GT+DF EF+ 
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72

Query: 251 MM 246
           MM
Sbjct: 73  MM 74


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 20/80 (25%), Positives = 42/80 (52%)
 Frame = -2

Query: 491 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXX 312
           +F++  +  + E ++E   +E  +AFR++DK+ +GYI T+   +                
Sbjct: 62  KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 311 LIQEIDTDGSGTVDFDEFME 252
           ++QE D   SG+ D+ +F++
Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138



 Score = 29.1 bits (62), Expect = 0.44
 Identities = 12/25 (48%), Positives = 18/25 (72%)
 Frame = -2

Query: 431 ELREAFRLYDKEGNGYIPTSSLREI 357
           E++EAF LYD + +G IPTS +  +
Sbjct: 10  EMKEAFVLYDIDKDGLIPTSHVGSV 34


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 35.9 bits (79), Expect = 0.004
 Identities = 23/89 (25%), Positives = 41/89 (46%), Gaps = 5/89 (5%)
 Frame = -2

Query: 491 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL-----REIXXXXXXXXXX 327
           EF+   + F V  + E   ++L+ AF++YD + +GYI    L       +          
Sbjct: 77  EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133

Query: 326 XXXXXLIQEIDTDGSGTVDFDEFMEMMTG 240
                 I E+D D  G + F+EF ++++G
Sbjct: 134 QIVDKTIMEVDKDRDGKISFEEFKDIVSG 162



 Score = 26.2 bits (55), Expect = 3.1
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = -2

Query: 299 IDTDGSGTVDFDEFMEMMT 243
           +D DG G VDF EF+  ++
Sbjct: 65  VDEDGGGDVDFQEFINSLS 83


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 33.9 bits (74), Expect = 0.016
 Identities = 16/71 (22%), Positives = 33/71 (46%)
 Frame = -2

Query: 455 EDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGT 276
           E  E  ++++ EAF+L+D + +  I    LR                 ++++ D  G G 
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89

Query: 275 VDFDEFMEMMT 243
           +  ++F+ +MT
Sbjct: 90  LQMEDFVRVMT 100


>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2280

 Score = 29.5 bits (63), Expect = 0.34
 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
 Frame = +3

Query: 258  ELVEVDGAASVSV-DLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFF 434
            EL  V   A  S  DL D AI   ++Q+L  F +DL +     VT+    +    +P ++
Sbjct: 1246 ELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYY 1305

Query: 435  LHGLS 449
             + +S
Sbjct: 1306 TYRVS 1310


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 0.34
 Identities = 15/60 (25%), Positives = 31/60 (51%)
 Frame = -2

Query: 425 REAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXLIQEIDTDGSGTVDFDEFMEMM 246
           ++AF L+D+ G G IP +S+ ++                I EI++     VD ++F++++
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDL----LRACGQNPTLAEITEIESTLPAEVDMEQFLQVL 64


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 11/18 (61%), Positives = 15/18 (83%)
 Frame = -2

Query: 434 KELREAFRLYDKEGNGYI 381
           +EL+EAF L DK+G+G I
Sbjct: 48  QELKEAFALLDKDGDGNI 65


>SPAC110.02 |pds5||cohesin-associated protein
           Pds5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1205

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +1

Query: 13  VDNIKYNSTTPDQLRTINYSLYNTYIILEKLADR 114
           V+N++YN   P +L T+      T   +EK+AD+
Sbjct: 747 VENLEYNGGLPVRLMTLGQLFLYTLEEVEKVADQ 780


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = -2

Query: 440 MQKELREAFRLYDKEGNGYIPTSSLR 363
           M +E  EAF L+D    GYI    LR
Sbjct: 10  MDEEAEEAFDLFDVTHKGYIDFEDLR 35


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -3

Query: 364  ARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 257
            A ++G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 968  ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003



 Score = 25.4 bits (53), Expect = 5.5
 Identities = 16/38 (42%), Positives = 21/38 (55%)
 Frame = -3

Query: 370  ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSS 257
            A A  +G+ TSS  T +   SS  S  T +A  +STSS
Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042


>SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease
           XPF|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 892

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 14/37 (37%), Positives = 22/37 (59%)
 Frame = -2

Query: 539 LLRRSTPTNLVVWNSPEFVTLAAKFIVEEDAEAMQKE 429
           LL  S P   +VW+S  +VT     I+ +D +AM++E
Sbjct: 774 LLTLSFPNLRIVWSSSAYVT----SIIFQDLKAMEQE 806


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = -2

Query: 494 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 393
           PEF   AA+ +VEED E +     EA  + D+EG
Sbjct: 38  PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68


>SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 12/40 (30%), Positives = 17/40 (42%)
 Frame = -3

Query: 373 RACARSSGNWTSS*LTMNSMASSKRSTLTEAAPSTSTSSW 254
           R C   S +W SS +T   +  S+R  L       S + W
Sbjct: 59  RLCKLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPW 98


>SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 396

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 15/44 (34%), Positives = 24/44 (54%)
 Frame = +1

Query: 10  SVDNIKYNSTTPDQLRTINYSLYNTYIILEKLADRYCFVAAN*C 141
           +V + KY ++  DQ  TI +   +TY  LEK+ D+   +  N C
Sbjct: 92  AVPSKKYTASF-DQSGTIGFDKESTYWKLEKILDKDLDMDVNYC 134


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,796,156
Number of Sequences: 5004
Number of extensions: 30618
Number of successful extensions: 131
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)