Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0023
         (765 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro...   144   2e-35
SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch...   144   2e-35
SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos...   142   4e-35
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po...    30   0.42 
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos...    27   2.2  
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb...    27   3.9  
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos...    26   6.8  
SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3 |Sc...    26   6.8  
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce...    25   9.0  

>SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal
           protein S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  144 bits (348), Expect = 2e-35
 Identities = 65/89 (73%), Positives = 75/89 (84%)
 Frame = +3

Query: 255 EKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDP 434
           EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV  G K VP+LVTHDGRTIRYPDP
Sbjct: 93  EKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDP 152

Query: 435 LIKVNDSIQLDIATTKIMDFIKFESGTCV 521
           LIKVND+I+L++ T KI  FIKF++   V
Sbjct: 153 LIKVNDTIKLNLETNKIESFIKFDTSAQV 181



 Score =  142 bits (343), Expect = 7e-35
 Identities = 62/87 (71%), Positives = 72/87 (82%)
 Frame = +1

Query: 1   HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
           HLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV  I+ Q
Sbjct: 8   HLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILMQ 67

Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSMRR 261
           RLIKVDGKVRTD T+P GFMDV+S+ +
Sbjct: 68  RLIKVDGKVRTDSTFPTGFMDVISVEK 94



 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 44/75 (58%), Positives = 56/75 (74%)
 Frame = +2

Query: 521 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNISLPRG 700
           M+TGGRN+GRVGTIV RE H GSF+I+H+KD+    FATRL+NVF+IG+  +  ISLP+G
Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGETGKSWISLPKG 241

Query: 701 KGXXXXXXXXERDKR 745
           KG        ERD+R
Sbjct: 242 KG-VKLSITEERDRR 255


>SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  144 bits (348), Expect = 2e-35
 Identities = 65/89 (73%), Positives = 75/89 (84%)
 Frame = +3

Query: 255 EKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDP 434
           EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV  G K VP+LVTHDGRTIRYPDP
Sbjct: 93  EKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDP 152

Query: 435 LIKVNDSIQLDIATTKIMDFIKFESGTCV 521
           LIKVND+I+L++ T KI  FIKF++   V
Sbjct: 153 LIKVNDTIKLNLETNKIESFIKFDTSAQV 181



 Score =  140 bits (339), Expect = 2e-34
 Identities = 61/87 (70%), Positives = 72/87 (82%)
 Frame = +1

Query: 1   HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
           HLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV  I+ Q
Sbjct: 8   HLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILMQ 67

Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSMRR 261
           RLI+VDGKVRTD T+P GFMDV+S+ +
Sbjct: 68  RLIQVDGKVRTDSTFPTGFMDVISVEK 94



 Score = 95.9 bits (228), Expect = 6e-21
 Identities = 45/75 (60%), Positives = 57/75 (76%)
 Frame = +2

Query: 521 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNISLPRG 700
           M+TGGRN+GRVGTIV RE H GSF+I+H+KD+    FATRL+NVF+IG+A +  ISLP+G
Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGEAGKSWISLPKG 241

Query: 701 KGXXXXXXXXERDKR 745
           KG        ERD+R
Sbjct: 242 KG-VKLSITEERDRR 255


>SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 262

 Score =  142 bits (345), Expect = 4e-35
 Identities = 64/89 (71%), Positives = 75/89 (84%)
 Frame = +3

Query: 255 EKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDP 434
           +KT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV  G K VP+LVTHDGRTIRYPDP
Sbjct: 93  DKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDP 152

Query: 435 LIKVNDSIQLDIATTKIMDFIKFESGTCV 521
           LIKVND+I+L++ T KI  FIKF++   V
Sbjct: 153 LIKVNDTIKLNLETNKIESFIKFDTSAQV 181



 Score =  141 bits (341), Expect = 1e-34
 Identities = 62/85 (72%), Positives = 71/85 (83%)
 Frame = +1

Query: 1   HLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQ 180
           HLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV  I+ Q
Sbjct: 8   HLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVKAILMQ 67

Query: 181 RLIKVDGKVRTDPTYPAGFMDVVSM 255
           RLIKVDGKVRTD T+P GFMDV+S+
Sbjct: 68  RLIKVDGKVRTDSTFPTGFMDVISV 92



 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 44/75 (58%), Positives = 56/75 (74%)
 Frame = +2

Query: 521 MITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKARRRNISLPRG 700
           M+TGGRN+GRVGTIV RE H GSF+I+H+KD+    FATRL+NVF+IG+  +  ISLP+G
Sbjct: 182 MVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGETGKSWISLPKG 241

Query: 701 KGXXXXXXXXERDKR 745
           KG        ERD+R
Sbjct: 242 KG-VKLSITEERDRR 255


>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1014

 Score = 29.9 bits (64), Expect = 0.42
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -3

Query: 697  ARQRDVTPSCLADYEHVVQPRGEGVSRGVLDVHNVEGAGMSLAGHD 560
            AR R +  S    Y  V+ P  EG     LD+H+   A ++L GH+
Sbjct: 888  ARLRSLFESYGKLYRVVLHPEHEGAVVEFLDIHDAGKASLALEGHE 933


>SPCC1450.11c |cek1||serine/threonine protein kinase
           Cek1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1338

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
 Frame = -1

Query: 234 SSRISRVSPNFPIN--LYEALFH-NFQDFVSGQSILQTIPQENHQGQALAQLVGT 79
           SS+   + P+ P++   +E  F  NFQ      S L  IP++   G ++A+++GT
Sbjct: 27  SSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGT 81


>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1428

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 8/15 (53%), Positives = 12/15 (80%)
 Frame = +2

Query: 440 QSQRFHPVRHCNYED 484
           ++++ HPVRH  YED
Sbjct: 5   ENEKIHPVRHSKYED 19


>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 640

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 20/86 (23%), Positives = 33/86 (38%)
 Frame = -3

Query: 313 WIVNLPLTS*IRRNSSLVFSLRQHP*IQQDK*GQS*LSHQPL*GVVSQFSGLRFRSKHTS 134
           W+V          + SL+      P ++  K G    + +   G +   + L  ++ HTS
Sbjct: 237 WLVEFSSVCQAAESISLIAVNNNRPPVELPKSGDVLSNREWEAGKIDAMNSLIAQNLHTS 296

Query: 133 DDSSGKSPGASTRATCGDRLTVSVHT 56
                 SP AST ++      V  HT
Sbjct: 297 ASQVSLSPMASTASSSVTNSPVDTHT 322


>SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 20/61 (32%), Positives = 24/61 (39%)
 Frame = +3

Query: 570 ARDIPAPSTLCTSRTPRDTPSPRG*TTCS*SARHEGVTSRCRAAKGIRXTIXRRSGTSAF 749
           ARD+   S           P  RG   C      E ++ RC A KGI     + S TSA 
Sbjct: 119 ARDLVVSSNSSLGHPILADPKSRGPLICKLRKEQE-ISLRCIAKKGIAKEHAKWSPTSAV 177

Query: 750 A 752
           A
Sbjct: 178 A 178


>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1261

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -2

Query: 335 SLLRGDTVDCESALNIID*TKQ 270
           S  RGDT D  +ALNI + TK+
Sbjct: 898 STRRGDTDDATTALNIFEETKR 919


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,244,948
Number of Sequences: 5004
Number of extensions: 69361
Number of successful extensions: 207
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 367316502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)