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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0017
         (797 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...   151   1e-37
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    95   1e-20
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    83   3e-17
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    81   2e-16
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    74   3e-14
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    72   8e-14
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    70   4e-13
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    69   6e-13
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    66   5e-12
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    63   5e-11
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    62   1e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    52   1e-07
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    47   3e-06
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    32   0.083
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ...    30   0.33 
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|...    28   1.8  
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos...    26   5.4  

>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score =  151 bits (366), Expect = 1e-37
 Identities = 62/88 (70%), Positives = 75/88 (85%)
 Frame = +2

Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLS 424
           ++ M W+  IPGK  T WEGGLYKL M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS
Sbjct: 36  LDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95

Query: 425 LLDEEKDWRPAITIKQILLGIQDLLNEP 508
           +L+EE+ W+PAITIKQILLGIQDLL++P
Sbjct: 96  ILNEEEGWKPAITIKQILLGIQDLLDDP 123



 Score = 57.2 bits (132), Expect = 3e-09
 Identities = 23/38 (60%), Positives = 28/38 (73%)
 Frame = +3

Query: 141 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNL 254
           MS +   RL EERK WR+DHPFGF A+P K+ DG L+L
Sbjct: 1   MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDL 38



 Score = 29.9 bits (64), Expect = 0.44
 Identities = 10/22 (45%), Positives = 18/22 (81%)
 Frame = +1

Query: 511 VKDPAQAEAYTIYCQNRLEYDK 576
           +  PAQ EAYT++ ++++EY+K
Sbjct: 125 IASPAQTEAYTMFKKDKVEYEK 146


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 94.7 bits (225), Expect = 1e-20
 Identities = 44/98 (44%), Positives = 56/98 (57%), Gaps = 11/98 (11%)
 Frame = +2

Query: 248 EFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 427
           +F TW+C I G  GTP+EGGLY   + F  DYP  PP  KFE   FHPNVY  GTVC+S+
Sbjct: 34  DFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISI 93

Query: 428 LDEEKD-----------WRPAITIKQILLGIQDLLNEP 508
           L    D           W P  ++++ILL +  +L EP
Sbjct: 94  LHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLAEP 131


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 83.4 bits (197), Expect = 3e-17
 Identities = 37/85 (43%), Positives = 49/85 (57%)
 Frame = +2

Query: 254 MTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLD 433
           M W   I G   TP+E G +KL + F + YP+ PP  KF   +FHPNVY +G +CL +L 
Sbjct: 34  MLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL- 92

Query: 434 EEKDWRPAITIKQILLGIQDLLNEP 508
            +  W P   +  IL  IQ LLN+P
Sbjct: 93  -QNRWSPTYDVAAILTSIQSLLNDP 116


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 80.6 bits (190), Expect = 2e-16
 Identities = 33/83 (39%), Positives = 52/83 (62%)
 Frame = +2

Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439
           W+  I G   +P+ GG++ L + F  DYP  PPK  F   ++HPN+  +G++CL +L ++
Sbjct: 33  WQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQ 92

Query: 440 KDWRPAITIKQILLGIQDLLNEP 508
             W PA+TI ++LL I  LL +P
Sbjct: 93  --WSPALTISKVLLSICSLLTDP 113


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 73.7 bits (173), Expect = 3e-14
 Identities = 32/83 (38%), Positives = 50/83 (60%)
 Frame = +2

Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439
           ++  + G + + +EGG + L +   D+YP  PP  +F   ++HPNV   G +CLS L  +
Sbjct: 34  FKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTL--K 91

Query: 440 KDWRPAITIKQILLGIQDLLNEP 508
           KDW PA+ I+ +LL IQ L+  P
Sbjct: 92  KDWSPALQIRTVLLSIQALMGAP 114


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 72.1 bits (169), Expect = 8e-14
 Identities = 34/83 (40%), Positives = 47/83 (56%)
 Frame = +2

Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439
           W   I G   T +EG  +K+ M F  +YP SPP   F  P++HPNV  SG +CL +L ++
Sbjct: 61  WAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDK 120

Query: 440 KDWRPAITIKQILLGIQDLLNEP 508
             W     ++ ILL +Q LL EP
Sbjct: 121 --WSAVYNVQTILLSLQSLLGEP 141


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 11/94 (11%)
 Frame = +2

Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLL--- 430
           WE  I G + T +EGG +   + F  DYP  PPK KF   ++HPNV+P+G VC+S+L   
Sbjct: 38  WEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPP 97

Query: 431 --------DEEKDWRPAITIKQILLGIQDLLNEP 508
                   D  + W P  + + IL+ +  +L+ P
Sbjct: 98  GDDKYGYEDAGERWLPVHSPETILISVISMLSSP 131


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 69.3 bits (162), Expect = 6e-13
 Identities = 31/78 (39%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
 Frame = +2

Query: 278 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRP 454
           G +GTP+EGG + + +    DYP  PPK  F+  ++HPNV   +G +CL +L ++  W P
Sbjct: 42  GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSP 99

Query: 455 AITIKQILLGIQDLLNEP 508
             T+K  L+ +Q LL  P
Sbjct: 100 VYTMKSALISLQSLLCTP 117


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 66.1 bits (154), Expect = 5e-12
 Identities = 28/71 (39%), Positives = 43/71 (60%)
 Frame = +2

Query: 296 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 475
           ++GG +K R+   D+YP  PPK K    ++HPN+   G VCL++L   +DW P + +  I
Sbjct: 65  YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122

Query: 476 LLGIQDLLNEP 508
           L+G+Q L   P
Sbjct: 123 LVGLQFLFLSP 133


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 62.9 bits (146), Expect = 5e-11
 Identities = 28/83 (33%), Positives = 46/83 (55%)
 Frame = +2

Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439
           W C   G   + + GG +   + F  DYP  PP  +F   ++HPN    G VCL++L ++
Sbjct: 39  WACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAIL-KQ 97

Query: 440 KDWRPAITIKQILLGIQDLLNEP 508
           + ++P+I ++ +L  I  LL EP
Sbjct: 98  QVFKPSIKLRSVLEQILQLLREP 120


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 29/87 (33%), Positives = 48/87 (55%), Gaps = 1/87 (1%)
 Frame = +2

Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 421
           ++   W+  I G   TP+EGG + L +   + YP SPP   F+  + HPN+ + +G VC+
Sbjct: 32  VDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCM 91

Query: 422 SLLDEEKDWRPAITIKQILLGIQDLLN 502
            +L  +  W PA +++   L I  LL+
Sbjct: 92  DIL--KTHWSPAWSLQSACLAIISLLS 116


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 27/79 (34%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
 Frame = +2

Query: 254 MTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLD 433
           + W   I G   TP+EGG Y   +IF  DYP  PP  +   P        +  +CLS  D
Sbjct: 35  LEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSFSD 92

Query: 434 -EEKDWRPAITIKQILLGI 487
              K W P+  +  IL+G+
Sbjct: 93  FHPKSWNPSWMVSTILVGL 111


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 25/79 (31%), Positives = 41/79 (51%), Gaps = 2/79 (2%)
 Frame = +2

Query: 278 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLSLLDEEKDWRP 454
           G   TP+ GG++K+ +    +YP   P   F   +FHPN+   SG+VCL ++++   W P
Sbjct: 38  GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQ--TWSP 95

Query: 455 AITIKQIL-LGIQDLLNEP 508
              +  I  + +  LL  P
Sbjct: 96  MFDMINIFEVFLPQLLRYP 114


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 32.3 bits (70), Expect = 0.083
 Identities = 26/97 (26%), Positives = 41/97 (42%), Gaps = 1/97 (1%)
 Frame = +2

Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 421
           I    W   I G   +  E  +Y L++    +YP +PP   F   +  P V   +G V  
Sbjct: 36  ITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNP 95

Query: 422 SLLDEEKDWRPAITIKQILLGIQDLLNEPT*KIQRKL 532
             +D  + W+   +++ +LL   DL  E      RKL
Sbjct: 96  HKIDCLRHWKREYSMETVLL---DLKKEMASSSNRKL 129


>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 509

 Score = 30.3 bits (65), Expect = 0.33
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = -3

Query: 369 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQVINS 247
           N  F G+     L  + S  SP  HG P +PG+ H+ + NS
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASLSNS 482


>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 574

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = -2

Query: 199 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 92
           W LR+  +S++  +    ++F K NSIY  +F++ L
Sbjct: 71  WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106


>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = +2

Query: 194 GPSLWFCREAYEESRWFIEFMTWECAIPGKKGTPWEGGLYK 316
           GPS    R+ +    + ++  TW C++ G+ G   +G L+K
Sbjct: 122 GPSKVVSRK-HAVVEYDLDDQTWNCSVYGRNGIKVDGKLFK 161


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,332,181
Number of Sequences: 5004
Number of extensions: 73073
Number of successful extensions: 171
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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