Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0028
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   191   1e-49
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   110   2e-25
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   106   3e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   100   4e-22

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  191 bits (465), Expect = 1e-49
 Identities = 82/132 (62%), Positives = 102/132 (77%)
 Frame = +2

Query: 47  MREIVHLQAGQCGNQIGAKXWEIISDEHGIXPTGAYHGDSDLQLERINVYYNEASGGKYV 226
           MREIVH+QAGQCGNQ+GA  W  I+DEHG+   G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 227 PRAILXDLEPGTXDSVRSGPXXQIFRPDNFVFXQSGAGINWAXGHYTXGAELXXSVLDAX 406
           PRA+L DLEPGT D+V+SG    +FRPDN ++ QSGAG  WA GHYT GAEL  +VLD  
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 407 RKESKSCNVPTG 442
           R+E+++C+   G
Sbjct: 121 RREAEACDALQG 132



 Score = 32.7 bits (71), Expect = 0.057
 Identities = 17/34 (50%), Positives = 18/34 (52%)
 Frame = +1

Query: 433 AYRAXQLTHSLXXXXXXXXGTLLXXKXREEXPXR 534
           A +  QLTHSL        GTLL  K REE P R
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDR 162


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  110 bits (264), Expect = 2e-25
 Identities = 52/130 (40%), Positives = 73/130 (56%), Gaps = 2/130 (1%)
 Frame = +2

Query: 47  MREIVHLQAGQCGNQIGAKXWEIISDEHGIXPTGAYHGDSDLQLER--INVYYNEASGGK 220
           MREI+ +  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 221 YVPRAILXDLEPGTXDSVRSGPXXQIFRPDNFVFXQSGAGINWAXGHYTXGAELXXSVLD 400
           YVPR+I  DLEP   D VR+GP   +F P+  +  +  A  N+A GHYT G EL   V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 401 AXRKESKSCN 430
             R+ + +C+
Sbjct: 121 KIRRIADNCS 130


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  106 bits (255), Expect = 3e-24
 Identities = 51/134 (38%), Positives = 74/134 (55%), Gaps = 6/134 (4%)
 Frame = +2

Query: 47  MREIVHLQAGQCGNQIGAKXWEIISDEHGIXPTG------AYHGDSDLQLERINVYYNEA 208
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 209 SGGKYVPRAILXDLEPGTXDSVRSGPXXQIFRPDNFVFXQSGAGINWAXGHYTXGAELXX 388
             GK+VPR+I  DLEP   D VR+GP   +F P+  V  +  A  N+A GHYT G E+  
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 389 SVLDAXRKESKSCN 430
           SVL+  R+ + +C+
Sbjct: 121 SVLERIRRMADNCS 134


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 99.5 bits (237), Expect = 4e-22
 Identities = 46/125 (36%), Positives = 76/125 (60%), Gaps = 2/125 (1%)
 Frame = +2

Query: 50  REIVHLQAGQCGNQIGAKXWEIISDEHGIXPTGAYHGDSDLQLERINVYYNEASGGKYVP 229
           REI+ LQAGQCGNQIG++ W+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 230 RAILXDLEPGTXDSVRSGPXXQIFRPDNFVFXQS--GAGINWAXGHYTXGAELXXSVLDA 403
           RAIL DLEP   +++ S     ++ P+N +  ++  GAG NWA G Y+    +   ++D 
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 404 XRKES 418
             +E+
Sbjct: 122 IDREA 126


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,917,028
Number of Sequences: 5004
Number of extensions: 25125
Number of successful extensions: 48
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)