Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0023
         (663 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F12.02c |p23fy||translationally controlled tumor protein ho...    69   5e-13
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...    30   0.26 
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    29   0.45 
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase...    26   5.6  
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    26   5.6  
SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce...    25   7.4  
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    25   9.7  

>SPAC1F12.02c |p23fy||translationally controlled tumor protein
           homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 168

 Score = 69.3 bits (162), Expect = 5e-13
 Identities = 32/66 (48%), Positives = 43/66 (65%)
 Frame = -2

Query: 446 KKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDG 267
           KKSY  Y+K YMK + A+L+E  P++V VF+ N    +K IL  FK+  F+ GESMD D 
Sbjct: 81  KKSYMSYIKGYMKAIKARLQESNPERVPVFEKNAIGFVKKILANFKDYDFYIGESMDPDA 140

Query: 266 MVAMWN 249
           MV + N
Sbjct: 141 MVVLMN 146



 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 25/54 (46%), Positives = 37/54 (68%), Gaps = 1/54 (1%)
 Frame = -1

Query: 663 GDEMFSDTYKMKLVDEVIYEVTGRLVTRAQG-DIQIEGFNPSAEEADEGTDSAS 505
           GDE+ SD Y +K VD+++YE   ++VT  QG D+ I G NPSAE+A+E  +  +
Sbjct: 10  GDELVSDAYDLKEVDDIVYEADCQMVTVKQGGDVDI-GANPSAEDAEENAEEGT 62



 Score = 26.2 bits (55), Expect = 4.2
 Identities = 14/26 (53%), Positives = 17/26 (65%)
 Frame = -1

Query: 255 VEYRDFDGTQIPIMMFFKHGLEEEKF 178
           + YR+ DG   P M+FFK GL  EKF
Sbjct: 145 MNYRE-DGIT-PYMIFFKDGLVSEKF 168


>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score = 30.3 bits (65), Expect = 0.26
 Identities = 17/40 (42%), Positives = 23/40 (57%)
 Frame = +2

Query: 137 ASLELLSYFLSNI*NFSSSRPCLKNIMIGICVPSKSLYST 256
           AS ELL+       NFS+ R CL+N ++  CVP   +Y T
Sbjct: 781 ASFELLNNLTEARKNFSNYRDCLENCVLP-CVPFLGVYFT 819


>SPCC162.08c |nup211||nuclear pore complex associated
            protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 29.5 bits (63), Expect = 0.45
 Identities = 13/49 (26%), Positives = 24/49 (48%)
 Frame = -2

Query: 389  EEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMVAMWNIE 243
            E+   +Q E  K+   +  K++     EL+    E++D DG   + N+E
Sbjct: 1479 EQITKEQFEQLKSEKERTEKELADSKNELEHLQSEAVDADGKTEISNLE 1527


>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
           Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1448

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
 Frame = +3

Query: 471 SLWFRTM-STPLSTPSPCPRQPLQPKD 548
           S + +T+ STP+ST SP   + L P+D
Sbjct: 844 SSYLKTLESTPMSTSSPTKEEKLFPRD 870


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 15/39 (38%), Positives = 18/39 (46%)
 Frame = +3

Query: 447 APKAYVSTSLWFRTMSTPLSTPSPCPRQPLQPKD*NPRS 563
           +P  Y+  S   R  ST   T  P PR PL P   N R+
Sbjct: 87  SPFPYLKGSFDDRFSSTHSLTRQPSPRSPLTPLKGNTRA 125


>SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 481

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 9/27 (33%), Positives = 17/27 (62%)
 Frame = +3

Query: 297 ELKFLKPAEDVFHYFVHVCFKYFNLVR 377
           +  FLKP   ++ YF+ +  +Y +L+R
Sbjct: 175 QFDFLKPNNALYPYFMRIVQQYTSLIR 201


>SPAC18G6.05c |||translation elongation regulator Gcn1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 9/28 (32%), Positives = 16/28 (57%)
 Frame = -2

Query: 449  CKKSYTLYLKDYMKKLVAKLEEKAPDQV 366
            CK  Y  Y+ D+++  +   E+K+ D V
Sbjct: 2165 CKLDYYRYVGDWVRHFITLFEDKSQDVV 2192


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,725,707
Number of Sequences: 5004
Number of extensions: 54729
Number of successful extensions: 165
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)