Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0006
         (609 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    71   1e-13
SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalyti...    37   0.002
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch...    37   0.002
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch...    27   1.6  
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ...    27   2.1  
SPAC5H10.06c |adh4||alcohol dehydrogenase Adh4|Schizosaccharomyc...    26   5.0  
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    25   6.5  
SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual     25   6.5  
SPBC11C11.02 |imp2||contractile ring protein Imp2|Schizosaccharo...    25   8.7  

>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 34/86 (39%), Positives = 52/86 (60%), Gaps = 1/86 (1%)
 Frame = +3

Query: 255  VALRLNSKNSRRFKKGDIVPYIICEDGTANSATQRAYHIEELKNSEH-LKVDFKYYLAHQ 431
            VAL+  ++     + GD++P+II           RAY  +E+ N+   L +D+ YYL+HQ
Sbjct: 1133 VALKKKARGEN-VRVGDVIPFIIAGSDADGHPADRAYSPQEIMNTNSTLVIDYNYYLSHQ 1191

Query: 432  LHPVISRICEPIEGMDPARVADCLGL 509
            + P I R+  PIEG + AR+A+CLGL
Sbjct: 1192 ILPPIERVIAPIEGTNRARLAECLGL 1217



 Score = 56.4 bits (130), Expect = 3e-09
 Identities = 29/82 (35%), Positives = 45/82 (54%)
 Frame = +1

Query: 10   RGLDIVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKDDLIGNKMPLSVLA 189
            +GLD+ R ++  LA EA KF L QILS +  +  +E+I ++L    + +   K P +   
Sbjct: 1051 KGLDMKRREFCTLAKEASKFCLDQILSGELTETVIENIHSYLMDFSEKMRNGKFPANKFI 1110

Query: 190  ITKQLTKNPNEYADKHTQSHVQ 255
            I  +L KNP +Y +  T   VQ
Sbjct: 1111 IFNRLGKNPEDYPNGKTMPFVQ 1132


>SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalytic
            subunit Cdc6 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1086

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 26/83 (31%), Positives = 44/83 (53%)
 Frame = +1

Query: 7    ARGLDIVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKDDLIGNKMPLSVL 186
            ++G++ VR D   L +    +++   L +   D  +E  Q    K+  DL+ NK+ +S L
Sbjct: 811  SKGIETVRRDNCPLVS----YVIDTALRKMLIDQDVEGAQLFTKKVISDLLQNKIDMSQL 866

Query: 187  AITKQLTKNPNEYADKHTQSHVQ 255
             ITK L+K   +YA K   +HV+
Sbjct: 867  VITKALSK--TDYAAK--MAHVE 885



 Score = 33.9 bits (74), Expect = 0.019
 Identities = 22/59 (37%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
 Frame = +3

Query: 300  GDIVPYIICEDGTANSATQRAYH-IEELKNSEHLKVDFKYYLAHQLHPVISRICEPIEG 473
            GD V Y+I +    +    R+   I  L+N+  + +D KYYL +QL   + RI EPI G
Sbjct: 902  GDRVAYVIIKGAQGDQFYMRSEDPIYVLENN--IPIDAKYYLENQLSKPLLRIFEPILG 958


>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
            Rev3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1480

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 21/67 (31%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
 Frame = +3

Query: 255  VALRLNSKNSRRFKK-GDIVPYIICEDGTANSATQRAYHIEELKNSEHLKVDFKYYLAHQ 431
            +A RL +K+ RR  + G+ VPY+I       +   R+   EE  +S   ++D  YY+ + 
Sbjct: 1268 MARRLMTKDPRREPQYGERVPYLIIAAAPGTTLANRSVAPEEFLSSSFSQLDINYYINNS 1327

Query: 432  LHPVISR 452
            L P + R
Sbjct: 1328 LIPPLDR 1334


>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2176

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 17/49 (34%), Positives = 24/49 (48%)
 Frame = +1

Query: 4   SARGLDIVRLDWSXLAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKD 150
           SA  L I  L  S L  +  K  + ++LS + P DR   + N  N+L D
Sbjct: 163 SAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNLGNRLTD 211


>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
           zf-fungal binuclear cluster type |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 977

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +1

Query: 106 DRLESIQNHLNKLKDDLIGNKMPLSVLAITKQL 204
           DR  S   HL  L ++ IG ++P+  L  TKQ+
Sbjct: 568 DRYASSHEHLQFLDENDIGIQLPVHELLFTKQI 600


>SPAC5H10.06c |adh4||alcohol dehydrogenase Adh4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 422

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
 Frame = +3

Query: 264 RLNSKNSR-RFKKGDIVPYIICEDGTANSATQR-AYHIEELKNSEHL 398
           + NS++ R   + GDI  ++ CE+ TA +A  R +  + E+K   HL
Sbjct: 333 KFNSRDPRANARLGDIAFHLGCEEHTAEAALDRISQLVLEVKIRPHL 379


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 15/46 (32%), Positives = 25/46 (54%)
 Frame = +1

Query: 46  LAAEAGKFILSQILSEQTPDDRLESIQNHLNKLKDDLIGNKMPLSV 183
           L+ E   +I   + S +T D+R+ESIQ    K    +I  K+P+ +
Sbjct: 22  LSEEWEPYIDKLVASNRTRDERVESIQELFQKC---VIEEKLPVDI 64


>SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 381

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 8/24 (33%), Positives = 14/24 (58%)
 Frame = -1

Query: 396 DVRNSLILLCGKPFAWLS*RFHLH 325
           D    L+L   +PF+W++ R  +H
Sbjct: 142 DSNGQLVLQLNRPFSWINSRLQIH 165


>SPBC11C11.02 |imp2||contractile ring protein
           Imp2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 670

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 12/56 (21%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
 Frame = +1

Query: 94  QTPDDRLESIQNHLNKLKDDLIGNKMPLSVLAITKQLTKNPNEYAD--KHTQSHVQ 255
           + PDD +E +Q +  + +  +  + MPL+   +     +N + + +   +T S +Q
Sbjct: 296 EVPDDSVELVQANFQRAQTKIENDNMPLNRPYVLSATARNESSFENTLPNTPSAIQ 351


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,433,591
Number of Sequences: 5004
Number of extensions: 48886
Number of successful extensions: 173
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)