Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060081.seq
         (575 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po...    84   1e-17
SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|...    28   1.1  
SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizo...    26   3.4  
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      26   3.4  
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    26   4.5  
SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    26   4.5  
SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac...    26   4.5  
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces...    26   4.5  

>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 942

 Score = 84.2 bits (199), Expect = 1e-17
 Identities = 39/76 (51%), Positives = 53/76 (69%)
 Frame = +1

Query: 40  LTGANLFRQEWSSLLDELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSA 219
           LTGA++FR +W  L+ EL +   IT +Q + Y+ L I G+VGSIDND   TD+TIG  S+
Sbjct: 301 LTGADIFRSDWPGLVKELEDTKAITPEQAKLYRHLTIVGLVGSIDNDMSSTDVTIGAFSS 360

Query: 220 LHRIIEAIDAIVALLI 267
           LHRI EA+D+I +  I
Sbjct: 361 LHRICEAVDSISSTAI 376



 Score = 61.7 bits (143), Expect = 7e-11
 Identities = 34/75 (45%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
 Frame = +3

Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVP-NNWVXKXXXXXXXXXX 431
           STA SH R FI+EVMGR+CG+ A++AALA+ A  VFIPE P     W  +          
Sbjct: 373 STAISHSRAFIVEVMGRHCGWLAVLAALATGADFVFIPERPAEVGKWQDELCNSLSSVRK 432

Query: 432 XXXXXNXIIXAEGAI 476
                + +I AEGAI
Sbjct: 433 LGKRKSIVIVAEGAI 447



 Score = 36.3 bits (80), Expect = 0.003
 Identities = 17/49 (34%), Positives = 26/49 (53%)
 Frame = +3

Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVPNNWVXK 401
           S + S +R F+ EV G   GY A V  L + A  ++ PED +  + + K
Sbjct: 743 SASASRRRVFVCEVQGGRSGYIATVGGLITGASAIYTPEDGISLDMLRK 791


>SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 270

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 9/30 (30%), Positives = 19/30 (63%)
 Frame = +2

Query: 92  CWRTTKLLKIKGKNINFCT*LEWLVQLTMI 181
           CW   +++K+ G  IN     +WL+++T++
Sbjct: 233 CWWINEVIKVNGSLINVWRRKQWLLEVTIM 262


>SPAC23G3.11 |rpn6||19S proteasome regulatory subunit
           Rpn6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 421

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
 Frame = +1

Query: 37  ELTGANLFRQEWSSLLDELLENNKI-TKDQREKYKFLHIAGMVG 165
           EL    + R   SSL D LLE N +   +   + +  HIA ++G
Sbjct: 310 ELASDPIIRSHLSSLYDNLLEQNLLRVVEPFSRVEVSHIAELIG 353


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 9/15 (60%), Positives = 12/15 (80%)
 Frame = +1

Query: 49  ANLFRQEWSSLLDEL 93
           AN+FR EWS+ LD +
Sbjct: 491 ANMFRNEWSNFLDSV 505


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 15/56 (26%), Positives = 27/56 (48%)
 Frame = +1

Query: 85  DELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSALHRIIEAIDAI 252
           +E  E  ++ + +R K    +      S DND    D T+G  +AL   I ++D++
Sbjct: 159 EESAERRRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTTVGIGAALAASILSVDSV 214


>SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 689

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 8/24 (33%), Positives = 15/24 (62%)
 Frame = +1

Query: 70  WSSLLDELLENNKITKDQREKYKF 141
           W +++D L E   + + ++E YKF
Sbjct: 326 WDAMIDSLYETINVEETKKETYKF 349


>SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase
           Alg6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 506

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = -3

Query: 153 SYVQKFIFFPLIFSNFVVLQQFI 85
           SY  KF+FFP +  + V +Q  I
Sbjct: 20  SYSSKFLFFPCLIMSLVFMQWLI 42


>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 446

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +1

Query: 40  LTGANLFRQEWSSLLDELLENNKITKDQREKY 135
           L G+  + +E  S +D+L+E  KI   +RE++
Sbjct: 41  LKGSKTWLEELESTIDDLIEVKKIGAKEREEH 72


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,280,040
Number of Sequences: 5004
Number of extensions: 41993
Number of successful extensions: 111
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)