SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060053.seq
         (634 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   115   5e-27
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   115   5e-27
SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr...    27   2.3  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   2.3  
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    26   3.9  
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra...    26   5.2  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  115 bits (277), Expect = 5e-27
 Identities = 48/54 (88%), Positives = 50/54 (92%)
 Frame = +1

Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417
           GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 59  GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112



 Score = 69.7 bits (163), Expect = 3e-13
 Identities = 32/58 (55%), Positives = 42/58 (72%)
 Frame = +2

Query: 80  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253
           ++ ARP VS+Y+ K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++
Sbjct: 1   MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 19/29 (65%), Positives = 22/29 (75%)
 Frame = +2

Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595
           IE+IPE+PLVV D VQ   KTK+AV  LK
Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLK 172



 Score = 34.7 bits (76), Expect = 0.011
 Identities = 17/60 (28%), Positives = 30/60 (50%)
 Frame = +3

Query: 450 LGGSVAATGVPALVQARGHILKRFPSFPWL*PTKSKXXXXXXXXXXXXXXLKAWSDILXV 629
           +  +VAA+GVP+L+ ARGH ++  P  P +     +              +KA+ D++ V
Sbjct: 124 IASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKV 183


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  115 bits (277), Expect = 5e-27
 Identities = 48/54 (88%), Positives = 50/54 (92%)
 Frame = +1

Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417
           GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 59  GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112



 Score = 70.5 bits (165), Expect = 2e-13
 Identities = 33/58 (56%), Positives = 42/58 (72%)
 Frame = +2

Query: 80  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253
           ++ ARP VS+YS K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++
Sbjct: 1   MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 19/29 (65%), Positives = 22/29 (75%)
 Frame = +2

Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595
           IE+IPE+PLVV D VQ   KTK+AV  LK
Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLK 172



 Score = 34.3 bits (75), Expect = 0.015
 Identities = 17/60 (28%), Positives = 30/60 (50%)
 Frame = +3

Query: 450 LGGSVAATGVPALVQARGHILKRFPSFPWL*PTKSKXXXXXXXXXXXXXXLKAWSDILXV 629
           +  +VAA+GVP+L+ ARGH ++  P  P +     +              +KA+ D++ V
Sbjct: 124 ISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKV 183


>SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 169

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = +2

Query: 545 DKVQEINKTKQAVIFLKAPQG 607
           DK+ +I KT Q+V FLK  QG
Sbjct: 55  DKIPDIRKTLQSVQFLKERQG 75


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 12/42 (28%), Positives = 17/42 (40%)
 Frame = +3

Query: 381 YVRPHEALAALAPSRQPPTAESGLGGSVAATGVPALVQARGH 506
           +  PH  L + AP  QP   E     +V     P+    +GH
Sbjct: 854 FYEPHSYLESPAPEPQPSYEEESFNATVIHAPTPSTATFQGH 895


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -2

Query: 141 APCTVSLFSEYTDTKGRATDRLISLL 64
           A C V ++SEYT   G ++  L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515


>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
           3-phosphatidyltransferase Pis1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%)
 Frame = -3

Query: 98  KVG-LPIDSFRYFSEAIPLKY-TLIAVI 21
           K+G LP+ SF Y +  +PL Y TL+AV+
Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,430,382
Number of Sequences: 5004
Number of extensions: 46882
Number of successful extensions: 135
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -