Silkworm BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0018
         (702 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    72   7e-14
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    57   3e-09
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    56   5e-09
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    52   8e-08
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    47   2e-06
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    42   9e-05
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    32   0.091
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    29   0.49 
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c...    29   0.85 
SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch...    28   1.5  
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2...    27   2.0  
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|...    26   4.5  
SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar...    26   6.0  
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    25   7.9  
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    25   7.9  

>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 72.1 bits (169), Expect = 7e-14
 Identities = 34/90 (37%), Positives = 51/90 (56%)
 Frame = +3

Query: 57  IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 236
           + DS + K+ + +  DKLVV+DF ATWCGPCK I PK ++  +                 
Sbjct: 5   VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLS 61

Query: 237 XXASEYNINSMPTFVFVKNGKKLDEFSGAN 326
             A+E  +++MP+F   KNG+K++E  GAN
Sbjct: 62  EIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 28/91 (30%), Positives = 47/91 (51%)
 Frame = +3

Query: 57  IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 236
           ++   D  TR++   DK+ V+DF A WCGPCK + P L+++ +E                
Sbjct: 22  VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78

Query: 237 XXASEYNINSMPTFVFVKNGKKLDEFSGANV 329
             A +  + ++PT V  + G++LD   GA+V
Sbjct: 79  DIAQKNGVYALPTMVLFRKGQELDRIVGADV 109


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 56.0 bits (129), Expect = 5e-09
 Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
 Frame = +3

Query: 111 VVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFV 287
           + +D  A WCGPCK I P   ++A++                   AS   + +MPTFVF 
Sbjct: 22  LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81

Query: 288 KNGKKLDEFSGAN 326
           +NGK++D  +GAN
Sbjct: 82  ENGKQIDMLTGAN 94


>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 52.0 bits (119), Expect = 8e-08
 Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
 Frame = +3

Query: 102 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTF 278
           DK++++ F A WCG CK + P+ +  A E+                   SEY+I   PT 
Sbjct: 39  DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98

Query: 279 VFVKNGKKLDEFSG 320
              KNGK++ ++SG
Sbjct: 99  NVFKNGKQISQYSG 112



 Score = 36.7 bits (81), Expect = 0.003
 Identities = 19/68 (27%), Positives = 31/68 (45%)
 Frame = +3

Query: 105 KLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 284
           K V+++F A WCG CK + P  +++A E                   S  +I+  PT +F
Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432

Query: 285 VKNGKKLD 308
            K   K++
Sbjct: 433 FKANDKVN 440


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 23/94 (24%), Positives = 42/94 (44%)
 Frame = +3

Query: 45  MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXX 224
           MS+ I   +  +  L    +++++++F A W  PCK +    D+ A +            
Sbjct: 1   MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEA 59

Query: 225 XXXXXXASEYNINSMPTFVFVKNGKKLDEFSGAN 326
                 A  +++N++P FV +   K L   SGAN
Sbjct: 60  EKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 25/94 (26%), Positives = 44/94 (46%), Gaps = 3/94 (3%)
 Frame = +3

Query: 51  IHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA--EMXXXXXXXXXXX 224
           + ++  ++L+  +  A  K  +I+F ATWCG CK + P  +E+ A  E            
Sbjct: 23  VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDA 81

Query: 225 XXXXXXASEYNINSMPTFV-FVKNGKKLDEFSGA 323
                 A +Y+I   PT + F  +G +  ++S A
Sbjct: 82  DTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115



 Score = 30.3 bits (65), Expect = 0.28
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +3

Query: 105 KLVVIDFMATWCGPCKMIGPKLDEI 179
           K V+++F A WCG CK + P  + +
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 31.9 bits (69), Expect = 0.091
 Identities = 22/90 (24%), Positives = 34/90 (37%), Gaps = 2/90 (2%)
 Frame = +3

Query: 63  DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX 242
           +S + +  +   G  LVV  F A WCG CK + P   ++A+ +                 
Sbjct: 37  NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNR 94

Query: 243 A--SEYNINSMPTFVFVKNGKKLDEFSGAN 326
           A  S+Y +   PT   V    K    S  +
Sbjct: 95  AVCSQYQVQGFPTIKLVYPSSKGSSLSSTD 124


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 29.5 bits (63), Expect = 0.49
 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 3/61 (4%)
 Frame = +3

Query: 138 CGPCKMIGPKLD---EIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFVKNGKKLD 308
           CG CK +GP  D   E A E                  +S  NI ++PT    +NG+ ++
Sbjct: 54  CGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVE 113

Query: 309 E 311
           E
Sbjct: 114 E 114


>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1687

 Score = 28.7 bits (61), Expect = 0.85
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = -1

Query: 483 KFITDVKNIFYSFIKNVYMFFFFNSILVSI 394
           KFI D +N F+  I   Y F  F ++L+ +
Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465


>SPAC1786.03 |cut11|SPAC24C9.01|integral membrane
           nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -2

Query: 488 SSNSLQTLKTFFIHLLKTYTCFFFLIP 408
           SS    +L  +F+ L++ + CFFF+ P
Sbjct: 50  SSGPFISLSFWFLSLVRGFVCFFFMFP 76


>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
           Mei2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 750

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = -1

Query: 507 QTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGC 385
           ++ + N   ++TD +NIF +F+ N   + F  + L  I  C
Sbjct: 71  KSSESNSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111


>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1402

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 17/45 (37%), Positives = 22/45 (48%)
 Frame = -1

Query: 528 FFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSI 394
           +F   +++T K     FI   KNIF  F     +FF F  IL SI
Sbjct: 84  WFPRNKIRTAKYTPIDFIP--KNIFLQFQNVANLFFLFLVILQSI 126


>SPMIT.02 |||mitochondrial DNA binding
           endonuclease|Schizosaccharomyces pombe|chr
           mitochondrial|||Manual
          Length = 384

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 14/61 (22%), Positives = 28/61 (45%)
 Frame = -1

Query: 546 ITTEPIFFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGCVQL*SL 367
           + T+ I+F   +  T  +   + +  +++ F S ++N+  FF       S G C+    L
Sbjct: 22  VITQLIYFLTSKKITN-LGKIRLVKSIRDSFLSQLENILCFFLVYRTTYSFGVCLMKRFL 80

Query: 366 F 364
           F
Sbjct: 81  F 81


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/22 (40%), Positives = 16/22 (72%)
 Frame = -2

Query: 497 KLISSNSLQTLKTFFIHLLKTY 432
           KL+SSN+LQ +  F+  ++K +
Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 16/59 (27%), Positives = 30/59 (50%)
 Frame = -2

Query: 356 FKDSCFEFVDVSAREFVQFLAILNEDERRHRVNVVLAGDVLALINVHLHNDDGI*HFGG 180
           F  + +E+VD ++ + + F+  L  +      ++   GD +  INV L + D   +FGG
Sbjct: 114 FSANLYEYVDGNS-DGISFVLNLENNNDTSVYHMTFHGDRVKPINVFLGSTDVTPNFGG 171


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,631,240
Number of Sequences: 5004
Number of extensions: 50824
Number of successful extensions: 184
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)