Silkworm BLAST Result against nematostella

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0065.Seq
         (620 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_6379| Best HMM Match : Proteasome (HMM E-Value=0)                   97   1e-20
SB_24524| Best HMM Match : Proteasome (HMM E-Value=1.4013e-45)         48   6e-06
SB_54379| Best HMM Match : AMP-binding (HMM E-Value=0.0023)            29   3.0  
SB_29551| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.3  
SB_38316| Best HMM Match : Syja_N (HMM E-Value=0.00032)                28   7.0  

>SB_6379| Best HMM Match : Proteasome (HMM E-Value=0)
          Length = 909

 Score = 97.1 bits (231), Expect = 1e-20
 Identities = 59/145 (40%), Positives = 73/145 (50%)
 Frame = +3

Query: 78  MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX*T 257
           MSI+ YNG A++AM G++CVAIA D+R GIQAQTVS    K    G              
Sbjct: 71  MSIMEYNGAAIIAMVGKNCVAIAADRRLGIQAQTVSCDFQKIFQMGEKLFVGLPGLATDV 130

Query: 258 QTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPYLWNLS*LA*IPYXNPTL 437
           QT+     FR NLY L+E    + K F  M+S LLY+RRFGPY          P  N   
Sbjct: 131 QTISSRLKFRLNLYELREGRPIKPKTFLSMVSNLLYERRFGPYFVEPVIAGLDPKTNEP- 189

Query: 438 MVCXMA*LVAQMTLKILWVSGPCSK 512
            +  M  +   M  K   VSG CS+
Sbjct: 190 FISSMDLIGCPMETKDFVVSGTCSE 214



 Score = 64.5 bits (150), Expect = 7e-11
 Identities = 34/74 (45%), Positives = 44/74 (59%)
 Frame = +2

Query: 347 VVNLIV*PTFRPLLMEPVIAGLDPLX*PNPYGLXHGLIGCPNDPEDFVGIGPLFEKLYGM 526
           V NL+    F P  +EPVIAGLDP     P+     LIGCP + +DFV  G   E++YGM
Sbjct: 161 VSNLLYERRFGPYFVEPVIAGLDPKT-NEPFISSMDLIGCPMETKDFVVSGTCSEQMYGM 219

Query: 527 CXALWGXPTXKPDE 568
           C +LW  P  +PD+
Sbjct: 220 CESLW-VPDLEPDD 232


>SB_24524| Best HMM Match : Proteasome (HMM E-Value=1.4013e-45)
          Length = 291

 Score = 48.0 bits (109), Expect = 6e-06
 Identities = 33/105 (31%), Positives = 44/105 (41%)
 Frame = +3

Query: 72  TNMSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX 251
           T  S  A+NGG V+A+ G+D   IA+D R     Q  +   PK        V   S    
Sbjct: 78  TYFSPYAFNGGTVLAISGEDFAVIASDTRLSQGFQIHTRDSPKVYKLTGSTVLGCSGFHG 137

Query: 252 *TQTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPY 386
              T+ +    R  +Y             + MLST+LY RRF PY
Sbjct: 138 DCLTLTKHISARLQMYEHDHGKAMSCTAIAQMLSTMLYYRRFFPY 182


>SB_54379| Best HMM Match : AMP-binding (HMM E-Value=0.0023)
          Length = 277

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 11/30 (36%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
 Frame = -1

Query: 491 PQNLQGHLGNQ--SSHXTDHKGWVXIGDLG 408
           P  ++G+L N   +++  DH+GW+  GD+G
Sbjct: 122 PLMMKGYLNNPEATANTIDHEGWLHTGDIG 151


>SB_29551| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 819

 Score = 28.3 bits (60), Expect = 5.3
 Identities = 9/38 (23%), Positives = 23/38 (60%)
 Frame = +1

Query: 355 PYCMTDVSALTYGTCHSWPRSPMXTQPLWSVXWLDWLP 468
           PYC   ++++ YG  H+W +  +  + L+++ +  ++P
Sbjct: 328 PYCQMMLNSVIYGDFHTWEKMGVTLKILFALLFTIFIP 365


>SB_38316| Best HMM Match : Syja_N (HMM E-Value=0.00032)
          Length = 567

 Score = 27.9 bits (59), Expect = 7.0
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = -1

Query: 527 TYHTAFRTGARYPQNLQGHLGNQSSHXTDHKGWV 426
           T++T + T  RY  N  G L NQ +H T +  WV
Sbjct: 381 THNTKYNTWVRYVIN--GVLANQVTHSTMYNTWV 412



 Score = 27.5 bits (58), Expect = 9.3
 Identities = 18/44 (40%), Positives = 22/44 (50%), Gaps = 3/44 (6%)
 Frame = -1

Query: 527 TYHTAFRTGARYPQNLQGHLGNQSSHXTDHKGWVXI---GDLGQ 405
           T+ T + T  RY  N  G L NQ +H T +  WV     G LGQ
Sbjct: 119 THSTMYNTWVRYVIN--GVLANQVTHSTMYNTWVLYVINGFLGQ 160


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,708,022
Number of Sequences: 59808
Number of extensions: 342118
Number of successful extensions: 628
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 624
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1536271375
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)