Silkworm BLAST Result against nematostella

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0023X.Seq
         (452 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_40561| Best HMM Match : Keratin_B2 (HMM E-Value=1.1)                30   1.0  

>SB_40561| Best HMM Match : Keratin_B2 (HMM E-Value=1.1)
          Length = 1139

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 24/66 (36%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
 Frame = -3

Query: 438  TTWRMCTTRLNGRYWMWTRS--LRSRWKSSPWMTA-SAHRATSETTWA--STHRRPAPRW 274
            TT  + TT  N R    T+S  L SR + +P  T  + HR   +T     S+ +RPAP  
Sbjct: 834  TTTPITTTITNHRLNCHTKSVILSSRKRPAPITTTITNHRLNCQTNSVILSSRKRPAPIT 893

Query: 273  STATSH 256
            +T T+H
Sbjct: 894  TTITNH 899



 Score = 28.7 bits (61), Expect = 2.4
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 5/66 (7%)
 Frame = -3

Query: 438 TTWRMCTTRLNGRYWMWTRS--LRSRWKSSPWMTA-SAHRATSETTWA--STHRRPAPRW 274
           TT  + TT  N R    T S  L SR + +P  T  + HR   +T     S+ +RPAP  
Sbjct: 224 TTTPITTTITNHRLNCHTSSVILSSRKRPAPITTTITNHRLNCQTNSVILSSRKRPAPIT 283

Query: 273 STATSH 256
           +T T+H
Sbjct: 284 TTITNH 289



 Score = 28.3 bits (60), Expect = 3.1
 Identities = 24/66 (36%), Positives = 32/66 (48%), Gaps = 5/66 (7%)
 Frame = -3

Query: 438 TTWRMCTTRLNGRYWMWTRS--LRSRWKSSPWMTA-SAHRATSETTWA--STHRRPAPRW 274
           TT  + TT  N R    T S  L SR + +P  T  + HR    T     S+ +RPAP  
Sbjct: 450 TTTPITTTITNHRLNCHTNSGILSSRKRPAPITTTITNHRLNCHTNSVILSSRKRPAPIT 509

Query: 273 STATSH 256
           +T T+H
Sbjct: 510 TTITNH 515



 Score = 28.3 bits (60), Expect = 3.1
 Identities = 24/66 (36%), Positives = 32/66 (48%), Gaps = 5/66 (7%)
 Frame = -3

Query: 438 TTWRMCTTRLNGRYWMWTRS--LRSRWKSSPWMTA-SAHRATSETTWA--STHRRPAPRW 274
           TT  + TT  N R    T S  L SR + +P  T  + HR    T     S+ +RPAP  
Sbjct: 598 TTTPITTTITNHRLNCHTNSGILSSRKRPAPITTTITNHRLNCHTNSVILSSRKRPAPIT 657

Query: 273 STATSH 256
           +T T+H
Sbjct: 658 TTITNH 663



 Score = 27.9 bits (59), Expect = 4.2
 Identities = 24/66 (36%), Positives = 32/66 (48%), Gaps = 5/66 (7%)
 Frame = -3

Query: 438 TTWRMCTTRLNGRYWMWTRS--LRSRWKSSPWMTA-SAHRATSETTWA--STHRRPAPRW 274
           TT  + TT  N R    T S  L SR + +P  T  + HR    T     S+ +RPAP  
Sbjct: 364 TTTPITTTITNHRLNCHTSSVILSSRKRPAPITTTITNHRLNCHTNSVILSSRKRPAPIT 423

Query: 273 STATSH 256
           +T T+H
Sbjct: 424 TTITNH 429


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,325,958
Number of Sequences: 59808
Number of extensions: 209249
Number of successful extensions: 567
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 564
length of database: 16,821,457
effective HSP length: 76
effective length of database: 12,276,049
effective search space used: 908427626
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)