Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0002
(700 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0) 114 7e-26
SB_4600| Best HMM Match : No HMM Matches (HMM E-Value=.) 64 1e-10
SB_33930| Best HMM Match : Ligase_CoA (HMM E-Value=0) 44 9e-05
SB_10131| Best HMM Match : Ligase_CoA (HMM E-Value=0) 44 9e-05
SB_52861| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.68
SB_57888| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.90
SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077) 31 1.2
SB_564| Best HMM Match : TrkA_N (HMM E-Value=0.05) 29 3.6
SB_47033| Best HMM Match : WAP (HMM E-Value=1.8e-38) 28 8.4
SB_29154| Best HMM Match : RNA_pol_Rpb2_7 (HMM E-Value=2.3) 28 8.4
>SB_54338| Best HMM Match : Ligase_CoA (HMM E-Value=0)
Length = 445
Score = 114 bits (274), Expect = 7e-26
Identities = 49/73 (67%), Positives = 58/73 (79%)
Frame = +2
Query: 479 RLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVG 658
RL+GPNCPG+I P +CKIGIMP +H G +G+VSRSGTLTYEA QTT G GQ+ CVG
Sbjct: 14 RLIGPNCPGVITPGECKIGIMPGHIHLPGKVGIVSRSGTLTYEAVKQTTDYGFGQSTCVG 73
Query: 659 IGGDPFNGTDFID 697
IGGDP G++FID
Sbjct: 74 IGGDPIPGSNFID 86
>SB_4600| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1140
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/57 (57%), Positives = 38/57 (66%), Gaps = 15/57 (26%)
Frame = +2
Query: 575 VVSRSGTLTYEACHQTTITGLGQTLCV---------------GIGGDPFNGTDFIDC 700
+VSRSGTLTYEA HQT+ LGQ+LCV GIGGDPFNGT+F+DC
Sbjct: 2 IVSRSGTLTYEAVHQTSHVNLGQSLCVVNNESCVYNYLFLPEGIGGDPFNGTNFVDC 58
>SB_33930| Best HMM Match : Ligase_CoA (HMM E-Value=0)
Length = 755
Score = 44.4 bits (100), Expect = 9e-05
Identities = 33/107 (30%), Positives = 52/107 (48%), Gaps = 8/107 (7%)
Frame = +2
Query: 401 IVCITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIG--------IMPAAVH 556
I I EG+P+ R+ + ++ ++GP G I P KIG I+ + ++
Sbjct: 144 IAIIAEGIPEAK-TRILNKKAKELGITIIGPATVGGIKPGCFKIGNTGGMLDNILASKLY 202
Query: 557 KRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFID 697
+ G + VSRSG ++ E + T G + IGGD + GT FID
Sbjct: 203 RPGSVAYVSRSGGMSNELNNIIARTTDGVYEGIAIGGDRYPGTTFID 249
>SB_10131| Best HMM Match : Ligase_CoA (HMM E-Value=0)
Length = 609
Score = 44.4 bits (100), Expect = 9e-05
Identities = 33/107 (30%), Positives = 52/107 (48%), Gaps = 8/107 (7%)
Frame = +2
Query: 401 IVCITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIG--------IMPAAVH 556
I I EG+P+ R+ + ++ ++GP G I P KIG I+ + ++
Sbjct: 293 IAIIAEGIPEAK-TRILNKKAKELGITIIGPATVGGIKPGCFKIGNTGGMLDNILASKLY 351
Query: 557 KRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFID 697
+ G + VSRSG ++ E + T G + IGGD + GT FID
Sbjct: 352 RPGSVAYVSRSGGMSNELNNIIARTTDGVYEGIAIGGDRYPGTTFID 398
>SB_52861| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1487
Score = 31.5 bits (68), Expect = 0.68
Identities = 20/74 (27%), Positives = 30/74 (40%), Gaps = 1/74 (1%)
Frame = -1
Query: 532 NFTLLRSNDTRTVRTNQSRLVLSQEGMFYSHHIMLWHTFCNAHNEGHFSLNSF*NGC-CS 356
NF+L D + LV+ ++G + C GHF L + + C C+
Sbjct: 337 NFSLSLDPDQVVQWLTRGPLVIDEDGECLCGNFSTG-LLCKHCVPGHFFLQGYCSRCQCN 395
Query: 355 SRGRYINNRGSCSC 314
G + RG CSC
Sbjct: 396 GHGSICDERGKCSC 409
>SB_57888| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 565
Score = 31.1 bits (67), Expect = 0.90
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH S NWYH S + W H S
Sbjct: 265 NWYHPSKTQANWYHPSKTQSNWNHPS 290
Score = 30.3 bits (65), Expect = 1.6
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH + NWYH S + W H S
Sbjct: 255 NWYHPTKTQANWYHPSKTQANWYHPS 280
Score = 30.3 bits (65), Expect = 1.6
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH S NWYH + + W H S
Sbjct: 465 NWYHPSKTQANWYHPTKTQANWYHPS 490
Score = 29.5 bits (63), Expect = 2.7
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLH 570
+WYH + NWYH S + W H
Sbjct: 475 NWYHPTKTQANWYHPSKTKANWYH 498
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH + NWYH + + W H S
Sbjct: 145 NWYHRTKTQANWYHPTKTQANWYHPS 170
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH + NWYH + + W H S
Sbjct: 245 NWYHPTKTQANWYHPTKTQANWYHPS 270
Score = 28.7 bits (61), Expect = 4.8
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLH 570
+WYH S NWYH + W H
Sbjct: 485 NWYHPSKTKANWYHPIKTQSNWYH 508
Score = 28.3 bits (60), Expect = 6.3
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLH 570
+WYH + NWYH + + W H
Sbjct: 505 NWYHPTKTQANWYHPTKTQANWYH 528
Score = 28.3 bits (60), Expect = 6.3
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLH 570
+WYH + NWYH + + W H
Sbjct: 515 NWYHPTKTQANWYHPTKTQANWCH 538
>SB_46293| Best HMM Match : Keratin_B2 (HMM E-Value=0.00077)
Length = 677
Score = 30.7 bits (66), Expect = 1.2
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH + NWYH S + W H S
Sbjct: 500 NWYHPTKTQANWYHPSKTQSNWYHPS 525
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTWLHRS 576
+WYH + NWYH + + W H S
Sbjct: 490 NWYHPTKTQCNWYHPTKTQANWYHPS 515
>SB_564| Best HMM Match : TrkA_N (HMM E-Value=0.05)
Length = 226
Score = 29.1 bits (62), Expect = 3.6
Identities = 19/35 (54%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 152 KVIVQGFTGKQGTFHSQQALDYGTKV-VGGVSPKK 253
KV+V G TGK G QQALD G V V SP+K
Sbjct: 9 KVVVFGGTGKTGLHVVQQALDRGHHVTVIARSPEK 43
>SB_47033| Best HMM Match : WAP (HMM E-Value=1.8e-38)
Length = 667
Score = 27.9 bits (59), Expect = 8.4
Identities = 16/70 (22%), Positives = 33/70 (47%)
Frame = +2
Query: 434 DMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEAC 613
D+ + + +QNK + + CP ++AP C+ ++ + IG+ S T +
Sbjct: 47 DVTKTSTHVQQQNKKKDM---CPFLLAPISCEKPVIECSSDIHCSIGMKCCSDTCNSKCV 103
Query: 614 HQTTITGLGQ 643
T+ G+G+
Sbjct: 104 ESQTLEGIGR 113
>SB_29154| Best HMM Match : RNA_pol_Rpb2_7 (HMM E-Value=2.3)
Length = 416
Score = 27.9 bits (59), Expect = 8.4
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +1
Query: 499 SWYHCS*EV*NWYHASCSAQTW 564
SWYHCS H CSA +W
Sbjct: 215 SWYHCSKIPDKERHQFCSANSW 236
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,728,987
Number of Sequences: 59808
Number of extensions: 496746
Number of successful extensions: 1290
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1280
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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