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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060060.seq
         (685 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_56821| Best HMM Match : His_leader (HMM E-Value=0.41)               31   0.66 
SB_13250| Best HMM Match : Toxin_22 (HMM E-Value=1.2)                  31   0.87 
SB_1879| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   2.0  
SB_8363| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   4.6  
SB_32896| Best HMM Match : F5_F8_type_C (HMM E-Value=8.6e-17)          28   6.1  
SB_33678| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.1  

>SB_56821| Best HMM Match : His_leader (HMM E-Value=0.41)
          Length = 131

 Score = 31.5 bits (68), Expect = 0.66
 Identities = 19/51 (37%), Positives = 22/51 (43%), Gaps = 1/51 (1%)
 Frame = -3

Query: 635 HHNHFGSNE*IFFTASEQTRHX-FGGHASMHYSVHISYHTKFIDHHPTASL 486
           HH H  S   I  T S  T    +  H   H +VH  YH     HHP +SL
Sbjct: 29  HHQHHPSIIIIIITISTITLVLHYHHHHRQHITVHHHYHHYHHHHHPPSSL 79


>SB_13250| Best HMM Match : Toxin_22 (HMM E-Value=1.2)
          Length = 376

 Score = 31.1 bits (67), Expect = 0.87
 Identities = 19/60 (31%), Positives = 28/60 (46%)
 Frame = -2

Query: 273 DARRFAVLNQWERLLMESPASIILTASSKVQLPIFKRA*RSRPALCTTYRLRTILIGIPE 94
           D    +V +  ER +   P S IL +    +L  FK     +  +CTT R ++  IGI E
Sbjct: 240 DITEISVTDALERTITRQPTSRILASWCPNKLEPFKHRDTGKEKVCTTKRRKSFDIGILE 299


>SB_1879| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 147

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 19/39 (48%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
 Frame = -1

Query: 610 NKFFSPLXNKRGTNSAGTL--RCIILCT*VITQNLLTTI 500
           NK F     +  TNS  TL  RCIILC   + QN LT I
Sbjct: 11  NKSFFENAGRDTTNSTVTLPQRCIILCIRDVQQNRLTCI 49


>SB_8363| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 776

 Score = 28.7 bits (61), Expect = 4.6
 Identities = 24/96 (25%), Positives = 40/96 (41%)
 Frame = -3

Query: 563 GHASMHYSVHISYHTKFIDHHPTASLLGLNTLAMCSSFPFLMKCSKRPKIICKQPNMS*E 384
           GH S  Y      H   +D      L   +T ++C S    M    R  I+C+    +  
Sbjct: 334 GHTS--YVSRSVIHRLSVDRSYIVCLSIGHTQSVCRSVIHRMSVD-RSYIVCQSIGYTSY 390

Query: 383 ISRSKLFKENICRPV*TRTLTGTTASICSNTLRTLS 276
           +SRS + + ++ R        G T+S+C + +  LS
Sbjct: 391 VSRSVIHRLSVDRSYTVSPSIGHTSSVCRSVIHRLS 426


>SB_32896| Best HMM Match : F5_F8_type_C (HMM E-Value=8.6e-17)
          Length = 278

 Score = 28.3 bits (60), Expect = 6.1
 Identities = 17/75 (22%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
 Frame = -3

Query: 638 NHHNHFGSNE*IFFTASEQTRHXFGGHA--SMHYSVHISYHTKFIDHHPTASLLGLNTLA 465
           ++H+H+  N+  +F      RH     A    +Y    +   ++ D      +L +NT+A
Sbjct: 59  HYHHHYRHND-HYFHQDHLYRHYLSTSALYEYYYKGRKNVFGRYSDRKQRVFIL-INTIA 116

Query: 464 MCSSFPFLMKCSKRP 420
             + FP++  C   P
Sbjct: 117 TLADFPYISFCRNTP 131


>SB_33678| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1308

 Score = 27.9 bits (59), Expect = 8.1
 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
 Frame = -3

Query: 482 GLNTLAMCSSFP-FLMKCSKRPKIICKQPN 396
           G+ ++A C   P  LM C+K  +I+C  PN
Sbjct: 263 GILSIAWCPQDPDLLMSCAKDNRILCWNPN 292


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,146,635
Number of Sequences: 59808
Number of extensions: 447244
Number of successful extensions: 1089
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1020
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1089
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1769412099
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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