Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30082
(859 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 142 4e-34
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 142 4e-34
At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 140 8e-34
At3g46840.1 68416.m05084 subtilase family protein contains simil... 32 0.56
At1g22470.1 68414.m02808 expressed protein 32 0.56
At4g37560.1 68417.m05316 formamidase, putative / formamide amido... 29 5.2
At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein si... 29 5.2
At4g26610.1 68417.m03835 protein kinase, putative similar to pro... 28 6.9
At3g04430.1 68416.m00469 no apical meristem (NAM) family protein... 28 6.9
At3g02090.2 68416.m00175 mitochondrial processing peptidase beta... 28 6.9
At3g02090.1 68416.m00174 mitochondrial processing peptidase beta... 28 6.9
At5g59090.1 68418.m07403 subtilase family protein contains simil... 28 9.1
At2g15670.1 68415.m01794 hypothetical protein 28 9.1
>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 435
Score = 142 bits (343), Expect = 4e-34
Identities = 66/82 (80%), Positives = 77/82 (93%)
Frame = +1
Query: 13 SADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAES 192
+ +PNV+GLILAGSADFKTELSQS++FDPRLQAKI+ +VDVSYGGENGFNQAIEL+AE
Sbjct: 215 ATSQPNVSGLILAGSADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEI 274
Query: 193 LQNVKFIQEKKLIGRYFDEISR 258
L NVKFIQEKKLIG+YF+EIS+
Sbjct: 275 LSNVKFIQEKKLIGKYFEEISQ 296
Score = 112 bits (269), Expect = 3e-25
Identities = 52/89 (58%), Positives = 65/89 (73%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
QDTGKY FGV+DTL+ALE+G+VETLI WENLDI RY LK++ T + I HL +QE ++S
Sbjct: 296 QDTGKYVFGVEDTLKALEMGAVETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQS 355
Query: 435 HFTDKESGVELELVECQPLLEWLAKTTSR 521
+F D E+ ELE+ E PLLEW A R
Sbjct: 356 NFHDAETNAELEVQEKMPLLEWFANEYKR 384
Score = 45.2 bits (102), Expect = 6e-05
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +2
Query: 512 YKSFGATLEIITDKSQEGSQFVRGF 586
YK FG TLE +T+KSQEGSQF RGF
Sbjct: 382 YKRFGCTLEFVTNKSQEGSQFCRGF 406
>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 434
Score = 142 bits (343), Expect = 4e-34
Identities = 66/82 (80%), Positives = 77/82 (93%)
Frame = +1
Query: 13 SADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAES 192
+ +PNV+GLILAGSADFKTELSQS++FDPRLQAKI+ +VDVSYGGENGFNQAIEL+AE
Sbjct: 214 ATSQPNVSGLILAGSADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEI 273
Query: 193 LQNVKFIQEKKLIGRYFDEISR 258
L NVKFIQEKKLIG+YF+EIS+
Sbjct: 274 LSNVKFIQEKKLIGKYFEEISQ 295
Score = 111 bits (266), Expect = 8e-25
Identities = 50/89 (56%), Positives = 62/89 (69%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
QDTGKY FGV+DTL+ALE+G++ETLI WENLDI RY LK+ T + + H +QE D S
Sbjct: 295 QDTGKYVFGVEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTS 354
Query: 435 HFTDKESGVELELVECQPLLEWLAKTTSR 521
+F D E+ ELE+ E PLLEW A R
Sbjct: 355 NFHDSETNAELEVQEKMPLLEWFANEYKR 383
Score = 45.2 bits (102), Expect = 6e-05
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +2
Query: 512 YKSFGATLEIITDKSQEGSQFVRGF 586
YK FG TLE +T+KSQEGSQF RGF
Sbjct: 381 YKRFGCTLEFVTNKSQEGSQFCRGF 405
>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
peptide chain release factor subunit 1-1 (eRF1-1)
(Eukaryotic release factor 1-1) (Omnipotent suppressor
protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
thaliana}, eukaryotic release factor 1 homolog
GI:1155261 from [Arabidopsis thaliana]; contains Pfam
profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
PF03465 eRF1 domain 3
Length = 436
Score = 140 bits (340), Expect = 8e-34
Identities = 66/82 (80%), Positives = 76/82 (92%)
Frame = +1
Query: 13 SADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAES 192
+ +PNV+GLILAGSADFKTELSQSDMFDPRL AKI+ +VDVSYGGENGFNQAIEL+AE
Sbjct: 216 ATSQPNVSGLILAGSADFKTELSQSDMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEI 275
Query: 193 LQNVKFIQEKKLIGRYFDEISR 258
L NVKFIQEK+LIG+YF+EIS+
Sbjct: 276 LANVKFIQEKRLIGKYFEEISQ 297
Score = 102 bits (244), Expect = 4e-22
Identities = 49/89 (55%), Positives = 61/89 (68%)
Frame = +3
Query: 255 QDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKS 434
QDTGKY FGV+DTL ALE G++ETLI WENLDI RYV+K+ AT + I HL EQE +
Sbjct: 297 QDTGKYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTE 356
Query: 435 HFTDKESGVELELVECQPLLEWLAKTTSR 521
+F +S + L++ E LLEWLA R
Sbjct: 357 NFKVADSDLALDVEEKLSLLEWLANEYRR 385
Score = 41.9 bits (94), Expect = 5e-04
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +2
Query: 512 YKSFGATLEIITDKSQEGSQFVRGF 586
Y+ FG LE +T+KSQEGSQF RGF
Sbjct: 383 YRRFGCALEFVTNKSQEGSQFCRGF 407
>At3g46840.1 68416.m05084 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo];
Length = 738
Score = 31.9 bits (69), Expect = 0.56
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = +3
Query: 621 WTFQSDAASTDRGNSTNLITDNSTDLFKLGKHFEGNGAKFPRVFKKGRFPFC 776
W F A++T+R T ++ N + + F+ NG K+P V+ K C
Sbjct: 324 WMFTVAASNTNRAFVTKVVLGNGKTVGRSVNSFDLNGKKYPLVYGKSASSSC 375
>At1g22470.1 68414.m02808 expressed protein
Length = 146
Score = 31.9 bits (69), Expect = 0.56
Identities = 17/72 (23%), Positives = 36/72 (50%)
Frame = +1
Query: 223 KLIGRYFDEISRTQESIASVSMIHCALSNSDPSRR*SAGRTSTYKDTY*SRMPPTRKQFS 402
KL ++D S S + +M C+ ++S+ S+ S+Y + S + P + ++S
Sbjct: 64 KLSSEFYDYSSSMSSSSLAKTMSSCSSASSESESDFSSTAISSYYSSVSSCLSPLQHRYS 123
Query: 403 T*RLNKRRTNHI 438
+NK++ H+
Sbjct: 124 EIVVNKKKKKHV 135
>At4g37560.1 68417.m05316 formamidase, putative / formamide
amidohydrolase, putative similar to SP|Q50228
Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)
{Methylophilus methylotrophus}; contains Pfam profile
PF03069: Acetamidase/Formamidase family
Length = 452
Score = 28.7 bits (61), Expect = 5.2
Identities = 23/70 (32%), Positives = 31/70 (44%)
Frame = +3
Query: 609 PLQSWTFQSDAASTDRGNSTNLITDNSTDLFKLGKHFEGNGAKFPRVFKKGRFPFCLYPG 788
P W F AS DR N +TD+ K +FEG A P++ RFP +PG
Sbjct: 108 PGDEWGF---TASFDRENGGGFLTDHFPCATKAIWYFEGIYAYSPQI-PGVRFPGLTHPG 163
Query: 789 FAFLGLKPDR 818
+G P +
Sbjct: 164 --IIGTAPSK 171
>At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 554
Score = 28.7 bits (61), Expect = 5.2
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Frame = +3
Query: 102 ASPSQD---HKTGGRIIWRRKRFQSGYRAGCGILAECQVYTGEETNWTLL 242
A P+ D + ++ RRKR Y + IL ECQ G+ W LL
Sbjct: 486 APPTDDLVLYAIAASVLQRRKRIIERYNSMDEILRECQSMAGQLDVWKLL 535
>At4g26610.1 68417.m03835 protein kinase, putative similar to
protein kinase G11A [Oryza sativa] SWISS-PROT:P47997
Length = 506
Score = 28.3 bits (60), Expect = 6.9
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3
Query: 99 SASPSQDHKTGGRIIWRRKRFQSGYRAGC 185
S +PS D KTGG + + K QSG C
Sbjct: 46 SFAPSSDSKTGGEVKFNEKSDQSGKSNTC 74
>At3g04430.1 68416.m00469 no apical meristem (NAM) family protein
similar to CUC1 (GP:12060422) {Arabidopsis thaliana}
Length = 198
Score = 28.3 bits (60), Expect = 6.9
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 264 GKYCFGVDDTLRALELGSVETLICWEN 344
G YC G+D+ L++LE G L+ EN
Sbjct: 156 GDYCNGLDELLQSLEQGESSGLLFGEN 182
>At3g02090.2 68416.m00175 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 535
Score = 28.3 bits (60), Expect = 6.9
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +1
Query: 151 ENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISRTQESI 273
++ NQA+++ A+ LQN KF E++ I R D I R + +
Sbjct: 187 DSNVNQALDVLADILQNSKF--EEQRINRERDVILREMQEV 225
>At3g02090.1 68416.m00174 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 531
Score = 28.3 bits (60), Expect = 6.9
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +1
Query: 151 ENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISRTQESI 273
++ NQA+++ A+ LQN KF E++ I R D I R + +
Sbjct: 187 DSNVNQALDVLADILQNSKF--EEQRINRERDVILREMQEV 225
>At5g59090.1 68418.m07403 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]
Length = 736
Score = 27.9 bits (59), Expect = 9.1
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = +3
Query: 621 WTFQSDAASTDRGNSTNLITDNSTDLF-KLGKHFEGNGAKFPRVFKK 758
W F A++T+RG T ++ N L + F+ G K+P V+ K
Sbjct: 314 WIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGK 360
>At2g15670.1 68415.m01794 hypothetical protein
Length = 193
Score = 27.9 bits (59), Expect = 9.1
Identities = 11/23 (47%), Positives = 18/23 (78%), Gaps = 2/23 (8%)
Frame = +3
Query: 312 GSVETLICWENL--DIQRYVLKS 374
GSV+TL CW+ + ++Q YV++S
Sbjct: 76 GSVQTLYCWDGMRVNVQLYVVES 98
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,675,592
Number of Sequences: 28952
Number of extensions: 468043
Number of successful extensions: 1310
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1252
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1310
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1999652000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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