Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30119.Seq
(448 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B) ribo... 103 5e-23
At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C) 101 3e-22
At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A) 100 5e-22
At4g17250.1 68417.m02594 expressed protein 28 2.5
At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 28 2.5
At3g19400.2 68416.m02460 cysteine proteinase, putative non-conse... 28 2.5
At3g19400.1 68416.m02461 cysteine proteinase, putative non-conse... 28 2.5
At3g51220.1 68416.m05607 expressed protein contains Pfam doamin ... 27 4.4
At1g32900.1 68414.m04053 starch synthase, putative similar to st... 27 5.8
At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putat... 27 7.6
>At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B)
ribosomal protein L31, Nicotiana glutinosa, U23784
Length = 119
Score = 103 bits (247), Expect = 5e-23
Identities = 46/67 (68%), Positives = 57/67 (85%)
Frame = +3
Query: 60 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 239
E+KG+ EVVTREYT+NLH+RLH FKK+AP+AIKEIRKFAEK+MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVVTREYTINLHRRLHSCTFKKKAPKAIKEIRKFAEKEMGTKDVRVDVKLN 60
Query: 240 KFLWSRG 260
K +WS+G
Sbjct: 61 KQIWSKG 67
Score = 29.5 bits (63), Expect = 1.1
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +2
Query: 308 NDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 400
NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 84 NDDEDAKEEFFSLVTVAEIPAEGLSGLGTKIIE 116
>At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)
Length = 119
Score = 101 bits (241), Expect = 3e-22
Identities = 45/67 (67%), Positives = 56/67 (83%)
Frame = +3
Query: 60 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLN 239
E+KG+ EV+TREYT+NLH+RLH FKK+AP+AIKEIRKFAEK MGT D+RVD +LN
Sbjct: 3 EKKGRK--EEVITREYTINLHRRLHKCTFKKKAPKAIKEIRKFAEKAMGTKDVRVDVKLN 60
Query: 240 KFLWSRG 260
K +WS+G
Sbjct: 61 KQIWSKG 67
Score = 29.5 bits (63), Expect = 1.1
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +2
Query: 308 NDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 400
NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 84 NDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIE 116
>At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A)
Length = 119
Score = 100 bits (239), Expect = 5e-22
Identities = 45/65 (69%), Positives = 52/65 (80%)
Frame = +3
Query: 66 KGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 245
KGK EVVTREYT+NLH+RLH FKK+AP AIKEIRKFA K MGT D+RVD +LNK
Sbjct: 3 KGKGRKEEVVTREYTINLHRRLHSCTFKKKAPNAIKEIRKFALKAMGTKDVRVDVKLNKQ 62
Query: 246 LWSRG 260
+WS+G
Sbjct: 63 IWSKG 67
Score = 29.5 bits (63), Expect = 1.1
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +2
Query: 308 NDDEDSAHKLFTLVTY--VPVASIKGLQTENVD 400
NDDED+ + F+LVT +P + GL T+ ++
Sbjct: 84 NDDEDAKEEFFSLVTVAEIPAEGLSGLGTKVIE 116
>At4g17250.1 68417.m02594 expressed protein
Length = 416
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +3
Query: 108 TVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVD 227
TV KR + +KKR A+K+ + E++ G P ++ D
Sbjct: 169 TVPATKRFLELKYKKRYEFALKQCPSYTERRRGVPKLKED 208
>At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profiles PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase, PF01593: amine oxidase, flavin-containing
Length = 867
Score = 28.3 bits (60), Expect = 2.5
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +3
Query: 171 KEIRKFAEKQMGTPDIRVDTRLNKFLWSRG-SEMF 272
+++ K+ E+ G PDI L +FL SRG SE+F
Sbjct: 128 EDVLKYIEELEGNPDIDRKETLGEFLNSRGYSELF 162
>At3g19400.2 68416.m02460 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 290
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +3
Query: 102 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 245
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At3g19400.1 68416.m02461 cysteine proteinase, putative
non-consensus AT acceptor site at exon 3; contains
similarity to cysteine protease CYP1 GI:2828252, TDI-65
GI:5726641 from [Lycopersicon esculentum]
Length = 362
Score = 28.3 bits (60), Expect = 2.5
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +3
Query: 102 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 245
++ V K +G+G K+R + K+ KF ++ PD + L +F
Sbjct: 46 QWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93
>At3g51220.1 68416.m05607 expressed protein contains Pfam doamin
PF05701: Plant protein of unknown function (DUF827)
Length = 186
Score = 27.5 bits (58), Expect = 4.4
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = +3
Query: 33 KITMAKPKGERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTP 212
K + K + E K S + E +T+E K H + P+ +++ E + P
Sbjct: 53 KENLKKAEEENKVLSQLIESLTQELETTKEKLNHSLRNFPEHPQVEDDLKFIEESTVNEP 112
Query: 213 DIRVDTRLNKF 245
D + ++N+F
Sbjct: 113 DNITEIKMNRF 123
>At1g32900.1 68414.m04053 starch synthase, putative similar to
starch synthase SP:Q42857 from [Ipomoea batatas]
Length = 610
Score = 27.1 bits (57), Expect = 5.8
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = +3
Query: 60 ERKGKSAINEVVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRV 224
E+KG + E +++ +N+ + G G KK + ++ KF K +G V
Sbjct: 412 EQKGSDILVEAISKFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNV 466
>At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profile PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase
Length = 867
Score = 26.6 bits (56), Expect = 7.6
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +3
Query: 171 KEIRKFAEKQMGTPDIRVDTRLNKFLWSRG-SEMF 272
+++ + EK G PDI L +FL +RG SE+F
Sbjct: 128 EDVLNYIEKLEGNPDIDRKETLGEFLNTRGYSELF 162
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,978,497
Number of Sequences: 28952
Number of extensions: 167040
Number of successful extensions: 341
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 341
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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