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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0006.Seq
         (765 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g23260.1 68417.m03353 protein kinase family protein contains ...    30   1.9  
At1g64405.1 68414.m07300 expressed protein                             28   5.9  
At1g66840.1 68414.m07597 expressed protein contains Pfam profile...    28   7.8  

>At4g23260.1 68417.m03353 protein kinase family protein contains
           Pfam domain PF00069: Protein kinase domain
          Length = 579

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 24/89 (26%), Positives = 47/89 (52%)
 Frame = -3

Query: 307 RKDQGRRKLPEWIVSFNLNKKM*S**LHNKQRNSVPEKGFEKFKEDINFNGSE*SLRRIM 128
           RK +    LP   V F+L K + S   +  +RN + + GF +  + +  NG+E +++R+ 
Sbjct: 232 RKQKQEMDLPTESVQFDL-KTIESATSNFSERNKLGKGGFGEVYKGMLMNGTEIAVKRLS 290

Query: 127 KELGFRWKKRTENNRKLVNEKSNIRLLRI 41
           K  G + +   +N   +V +  +I L+R+
Sbjct: 291 KTSG-QGEVEFKNEVVVVAKLQHINLVRL 318


>At1g64405.1 68414.m07300 expressed protein
          Length = 118

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 15/58 (25%), Positives = 33/58 (56%)
 Frame = +3

Query: 201 GTEFRCLLCSYYDYIFLLRLNETIHSGNFLLP*SFLTRSPKHGEQARAFSFVNNAPNS 374
           G + + +L  +   +FLL++N + H GN ++    +    K   +A +FS ++++P+S
Sbjct: 41  GMKVKIVLTRHELDMFLLQMNRS-HDGNLMITKDVMVELEKRIIRASSFSSLSSSPSS 97


>At1g66840.1 68414.m07597 expressed protein contains Pfam profile
           PF05701: Plant protein of unknown function (DUF827);
           expression supported by MPSS
          Length = 607

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 27/119 (22%), Positives = 56/119 (47%), Gaps = 2/119 (1%)
 Frame = -2

Query: 440 KQGKNDIEKLKQTSEATKTSVCTVRCIINEAKGSGLLTVFRTPGKKRSGKKKVTG-MDSF 264
           ++GK+++  LK+ +EAT+     +  I   A+   L+    T  K+    KK T  +D  
Sbjct: 265 ERGKDNLSVLKEVTEATEAKKAELASI--NAELFCLVNTMDTLRKEFDHAKKETAWLDKM 322

Query: 263 VQS**KDVIIITT*QAAKLRTGERLRKI*R-RYKF*WLGMKLTTNYERARLQMEKKNRK 90
           +Q   KD +++       L   ++L  + +   +  +L   LTT++E+ +   E   ++
Sbjct: 323 IQ---KDDVMLERLNTKLLIAKDQLEAVSKAEERISYLADNLTTSFEKLKSDREAAKKE 378


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,942,708
Number of Sequences: 28952
Number of extensions: 302332
Number of successful extensions: 761
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 761
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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