SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0071.Seq
         (518 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g18080.1 68414.m02238 WD-40 repeat family protein / auxin-dep...    56   1e-08
At1g48630.1 68414.m05440 guanine nucleotide-binding family prote...    56   2e-08
At3g18130.1 68416.m02305 guanine nucleotide-binding family prote...    55   3e-08
At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p...    29   1.9  
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea...    29   2.5  
At3g10530.1 68416.m01264 transducin family protein / WD-40 repea...    28   4.3  
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p...    28   4.3  
At3g59590.1 68416.m06649 jacalin lectin family protein similar t...    27   5.7  
At5g10940.1 68418.m01269 transducin family protein / WD-40 repea...    27   7.6  

>At1g18080.1 68414.m02238 WD-40 repeat family protein /
           auxin-dependent protein (ARCA) / guanine
           nucleotide-binding protein beta subunit, putative
           identical to SP|O24456 Guanine nucleotide-binding
           protein beta subunit-like protein (WD-40 repeat
           auxin-dependent protein ARCA) {Arabidopsis thaliana};
           contains 7 WD-40 repeats (PF00400)
          Length = 327

 Score = 56.4 bits (130), Expect = 1e-08
 Identities = 41/121 (33%), Positives = 57/121 (47%), Gaps = 10/121 (8%)
 Frame = -3

Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXLXLLXXQXLXX 337
           G  G+++T   SPDGSLCASGGK    + WDL +G  L++L+ N +I  L     +    
Sbjct: 192 GHTGYVSTVAVSPDGSLCASGGKDGVVLLWDLAEGKKLYSLEANSVIHALCFSPNR--YW 249

Query: 336 XCLRTFH----QDLGSGKQXDG*R------AQA*NH*QTXTSKTXPPQCLSLAWSTDGQT 187
            C  T H     DL S    +  +      A+  ++     +K     C SL WS DG T
Sbjct: 250 LCAATEHGIKIWDLESKSIVEDLKVDLKAEAEKADNSGPAATKRKVIYCTSLNWSADGST 309

Query: 186 L 184
           L
Sbjct: 310 L 310



 Score = 39.5 bits (88), Expect = 0.001
 Identities = 18/37 (48%), Positives = 23/37 (62%)
 Frame = -1

Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
           AL FS  R W   A    IKIWDL SK +VE+L+ ++
Sbjct: 240 ALCFSPNRYWLCAATEHGIKIWDLESKSIVEDLKVDL 276


>At1g48630.1 68414.m05440 guanine nucleotide-binding family protein
           / activated protein kinase C receptor, putative / RACK,
           putative contains 7 WD-40 repeats (PF00400); very
           similar to guanine nucleotide-binding protein; activated
           protein kinase C receptor; RACK1 (GI:9294068)
           {Arabidopsis thaliana}; similar to WD-40 repeat
           auxin-dependent protein ARCA (SP:O24456) [Arabidopsis
           thaliana];
          Length = 326

 Score = 55.6 bits (128), Expect = 2e-08
 Identities = 43/121 (35%), Positives = 56/121 (46%), Gaps = 10/121 (8%)
 Frame = -3

Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXLXLLXXQXLXX 337
           G  G+LNT   SPDGSLCASGGK    + WDL +G  L++L+   II  L     +    
Sbjct: 191 GHSGYLNTVAVSPDGSLCASGGKDGVILLWDLAEGKKLYSLEAGSIIHSLCFSPNR--YW 248

Query: 336 XCLRTFHQ----DLGSGKQXDG*R------AQA*NH*QTXTSKTXPPQCLSLAWSTDGQT 187
            C  T +     DL S    +  +      A+  +      +KT    C SL WS DG T
Sbjct: 249 LCAATENSIRIWDLESKSVVEDLKVDLKAEAEKTDGSTGIGNKTKVIYCTSLNWSADGNT 308

Query: 186 L 184
           L
Sbjct: 309 L 309



 Score = 38.7 bits (86), Expect = 0.002
 Identities = 17/37 (45%), Positives = 24/37 (64%)
 Frame = -1

Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
           +L FS  R W   A   SI+IWDL SK +VE+L+ ++
Sbjct: 239 SLCFSPNRYWLCAATENSIRIWDLESKSVVEDLKVDL 275


>At3g18130.1 68416.m02305 guanine nucleotide-binding family protein
           / activated protein kinase C receptor (RACK1) identical
           to guanine nucleotide-binding protein; activated protein
           kinase C receptor; RACK1 (GI:9294068) {Arabidopsis
           thaliana}; contains Pfam profile: PF00400 WD domain,
           G-beta repeat (7 copies)
          Length = 326

 Score = 54.8 bits (126), Expect = 3e-08
 Identities = 26/50 (52%), Positives = 32/50 (64%)
 Frame = -3

Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXL 367
           G  G+LNT   SPDGSLCASGGK    + WDL +G  L++L+   II  L
Sbjct: 191 GHSGYLNTVAVSPDGSLCASGGKDGVILLWDLAEGKKLYSLEAGSIIHSL 240



 Score = 38.7 bits (86), Expect = 0.002
 Identities = 17/37 (45%), Positives = 24/37 (64%)
 Frame = -1

Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
           +L FS  R W   A   SI+IWDL SK +VE+L+ ++
Sbjct: 239 SLCFSPNRYWLCAATENSIRIWDLESKSVVEDLKVDL 275


>At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p80
           subunit, putative similar to contains 6 WD-40 repeats
           (PF00400); katanin p80 subunit (GI:3005601)
           [Strongylocentrotus purpuratus]
          Length = 974

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 14/34 (41%), Positives = 16/34 (47%)
 Frame = -3

Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
           ++T   SPDG    SGG       WDL  G  LH
Sbjct: 94  ISTIEFSPDGRWVVSGGLDNVVKVWDLTAGKLLH 127


>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
           family protein similar to katanin p80 subunit
           [Strongylocentrotus purpuratus] GI:3005601; contains
           Pfam profile PF00400: WD domain, G-beta repeat
          Length = 871

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 13/34 (38%), Positives = 16/34 (47%)
 Frame = -3

Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
           +N    +PDG    SGG+      WDL  G  LH
Sbjct: 239 VNVLRFTPDGRWIVSGGEDNVVKVWDLTAGKLLH 272


>At3g10530.1 68416.m01264 transducin family protein / WD-40 repeat
           family protein contains 2 WD-40 repeats (PF00400); BING4
           (gi:3811380) {Mus musculus]; similar to hypothetical
           protein GB:P40055 [Saccharomyces cerevisiae]
          Length = 536

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 12/38 (31%), Positives = 19/38 (50%)
 Frame = -3

Query: 507 GHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTL 394
           G +++    P+G L A+ GK  K   WDL     + T+
Sbjct: 282 GPVSSVAFHPNGHLMATSGKERKIKIWDLRKFEEVQTI 319


>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
           subunit, putative contains 5 WD-40 repeats (PF00400);
           similar to katanin p80 subunit (GI:3005601)
           [Strongylocentrotus purpuratus]
          Length = 1180

 Score = 27.9 bits (59), Expect = 4.3
 Identities = 13/34 (38%), Positives = 16/34 (47%)
 Frame = -3

Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
           ++T   +PDG    SGG       WDL  G  LH
Sbjct: 145 ISTIRFTPDGRWVVSGGLDNVVKVWDLTAGKLLH 178


>At3g59590.1 68416.m06649 jacalin lectin family protein similar to
           suppressor of nim1-1 [Arabidopsis thaliana] GI:22023774;
           contains Pfam profile PF01419: Jacalin-like lectin
           domain
          Length = 454

 Score = 27.5 bits (58), Expect = 5.7
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -3

Query: 507 GHLNTXTXSPDGSLCASGGKYMKAM 433
           GH N  T +PD  +  +GG Y++A+
Sbjct: 350 GHGNATTHNPDDEIMIAGGDYIEAV 374


>At5g10940.1 68418.m01269 transducin family protein / WD-40 repeat
           family protein unnamed ORF cDNA FLJ10872, Homo sapiens,
           EMBL:AK001734; contains Pfam PF00400: WD domain, G-beta
           repeat (6 copies,1 weak)
          Length = 757

 Score = 27.1 bits (57), Expect = 7.6
 Identities = 12/42 (28%), Positives = 21/42 (50%)
 Frame = -3

Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLD 391
           G  G +N    + +GSL  SG   ++   W+ +    LH++D
Sbjct: 48  GHQGCVNALAWNSNGSLLISGSDDLRINIWNYSSRKLLHSID 89


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,564,339
Number of Sequences: 28952
Number of extensions: 124804
Number of successful extensions: 363
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 323
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -