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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0004.Seq
         (399 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g07810.1 68418.m00895 SNF2 domain-containing protein / helica...    27   3.5  
At5g04500.1 68418.m00449 glycosyltransferase family protein 47 l...    27   6.1  
At1g30130.2 68414.m03683 expressed protein similar to hypothetic...    27   6.1  
At1g30130.1 68414.m03682 expressed protein similar to hypothetic...    27   6.1  
At1g13900.1 68414.m01631 calcineurin-like phosphoesterase family...    27   6.1  

>At5g07810.1 68418.m00895 SNF2 domain-containing protein / helicase
            domain-containing protein / HNH endonuclease
            domain-containing protein similar to HepA-related protein
            HARP [Homo sapiens] GI:6693791; contains Pfam profiles
            PF00271: Helicase conserved C-terminal domain, PF00176:
            SNF2 family N-terminal domain, PF01844: HNH endonuclease
          Length = 1190

 Score = 27.5 bits (58), Expect = 3.5
 Identities = 10/17 (58%), Positives = 13/17 (76%)
 Frame = -1

Query: 306  RREQTFTQAWSRKNMPL 256
            R+E+ +TQAWS  N PL
Sbjct: 966  RKEKEYTQAWSMSNEPL 982


>At5g04500.1 68418.m00449 glycosyltransferase family protein 47 low
           similarity to Exostosin-like 2, Homo sapiens
           [SP|Q9UBQ6], EXTL2, Mus musculus [GI:10443633]
          Length = 765

 Score = 26.6 bits (56), Expect = 6.1
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -1

Query: 399 HNHFRQFFYLGRFKYIGRFLHFV*CF 322
           HNH  ++ Y+  +    RFL F  CF
Sbjct: 23  HNHHHRYKYISNYGVGRRFLFFASCF 48


>At1g30130.2 68414.m03683 expressed protein similar to hypothetical
           protein GI:1469227 from [Brassica oleracea]
          Length = 311

 Score = 26.6 bits (56), Expect = 6.1
 Identities = 17/52 (32%), Positives = 27/52 (51%)
 Frame = -3

Query: 385 AVLLPRAVQVHRTLPPLRLVFPGVIIQA*TDFYTSLVKEKYAFARKHIRYSL 230
           ++LLP    +HR LP    V P V     T ++  L   +++F R  +RY+L
Sbjct: 21  SLLLPFRFLLHRLLPSRAAVDPNVSFYEGTVWHDRLRPVRHSF-RYSVRYAL 71


>At1g30130.1 68414.m03682 expressed protein similar to hypothetical
           protein GI:1469227 from [Brassica oleracea]
          Length = 311

 Score = 26.6 bits (56), Expect = 6.1
 Identities = 17/52 (32%), Positives = 27/52 (51%)
 Frame = -3

Query: 385 AVLLPRAVQVHRTLPPLRLVFPGVIIQA*TDFYTSLVKEKYAFARKHIRYSL 230
           ++LLP    +HR LP    V P V     T ++  L   +++F R  +RY+L
Sbjct: 21  SLLLPFRFLLHRLLPSRAAVDPNVSFYEGTVWHDRLRPVRHSF-RYSVRYAL 71


>At1g13900.1 68414.m01631 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 calcineurin-like
           phosphoesterase
          Length = 656

 Score = 26.6 bits (56), Expect = 6.1
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = +2

Query: 59  SRYKTGAFYVC*LKLNKEKVIVKIVRSH 142
           S Y+TG F    L  NKEK+ V  V +H
Sbjct: 540 SMYRTGEFGYTRLVANKEKLTVSFVGNH 567


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,851,827
Number of Sequences: 28952
Number of extensions: 148536
Number of successful extensions: 263
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 258
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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