SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30014
         (383 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70...    70   5e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta...    68   2e-12
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS...    65   1e-11
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS...    65   1e-11
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS...    65   2e-11
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70...    64   4e-11
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)...    58   2e-09
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)...    58   2e-09
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)...    58   2e-09
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)...    52   1e-07
At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp...    31   0.26 
At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp...    29   1.4  
At5g01570.1 68418.m00072 hypothetical protein hypothetical prote...    28   2.5  
At3g56850.1 68416.m06322 ABA-responsive element-binding protein ...    28   2.5  
At2g01270.1 68415.m00040 thioredoxin family protein low similari...    27   3.3  
At3g19050.1 68416.m02420 kinesin motor protein-related contains ...    27   4.3  
At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing ...    27   4.3  
At2g41070.3 68415.m05073 basic leucine zipper transcription fact...    27   4.3  
At2g41070.2 68415.m05072 basic leucine zipper transcription fact...    27   4.3  
At2g41070.1 68415.m05071 basic leucine zipper transcription fact...    27   4.3  
At4g29790.1 68417.m04242 expressed protein                             26   9.9  
At3g62240.1 68416.m06992 zinc finger (C2H2 type) family protein ...    26   9.9  
At3g60000.1 68416.m06699 hypothetical protein  contains Pfam pro...    26   9.9  
At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low simi...    26   9.9  
At2g37870.1 68415.m04649 protease inhibitor/seed storage/lipid t...    26   9.9  

>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
           putative similar to heat shock protein hsp70 GI:1771478
           from [Pisum sativum]
          Length = 646

 Score = 70.1 bits (164), Expect = 5e-13
 Identities = 30/55 (54%), Positives = 43/55 (78%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           L +K++  DKQ I    ++TI+W++ NQLA+ +E+E+K KELEGI NPII+KMYQ
Sbjct: 563 LAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEFEYKLKELEGICNPIISKMYQ 617


>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
           putative / HSC70, putative / HSP70, putative strong
           similarity to heat shock cognate 70 kd protein 1
           SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
          Length = 617

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 28/53 (52%), Positives = 39/53 (73%)
 Frame = +1

Query: 7   EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           EK+  +DK+   D   + I+WLD NQLA+ +E+EHK KELE +++ IITKMYQ
Sbjct: 564 EKLPAADKKKFEDSIEEVIQWLDDNQLAEADEFEHKMKELESVWSTIITKMYQ 616


>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
           (HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
           cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
           thaliana}
          Length = 651

 Score = 65.3 bits (152), Expect = 1e-11
 Identities = 29/53 (54%), Positives = 37/53 (69%)
 Frame = +1

Query: 7   EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           EK+  +DK+ I D     I+WL+ NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 566 EKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQ 618


>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
           (HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
           cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
           thaliana}
          Length = 653

 Score = 65.3 bits (152), Expect = 1e-11
 Identities = 27/53 (50%), Positives = 37/53 (69%)
 Frame = +1

Query: 7   EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           EK+  +DK+ + D   + I+WLD NQL + +E+E K KELE + NPII KMYQ
Sbjct: 566 EKLPAADKKKVEDSIEEAIQWLDGNQLGEADEFEDKMKELESVCNPIIAKMYQ 618


>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
           (HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
           cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
           thaliana}
          Length = 649

 Score = 64.9 bits (151), Expect = 2e-11
 Identities = 29/53 (54%), Positives = 38/53 (71%)
 Frame = +1

Query: 7   EKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           EK++  DK+ I D     I+WL++NQLA+ +E+E K KELE I NPII KMYQ
Sbjct: 566 EKLAGDDKKKIEDSIEAAIEWLEANQLAECDEFEDKMKELESICNPIIAKMYQ 618


>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
           putative strong similarity to heat shock protein
           GI:425194 [Spinacia oleracea]
          Length = 650

 Score = 63.7 bits (148), Expect = 4e-11
 Identities = 27/55 (49%), Positives = 38/55 (69%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           +  K+  +DK+ I D  +  I+WLD NQLA+ +E+E K KELE + NPII +MYQ
Sbjct: 564 IASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQ 618


>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
           similar to SWISS-PROT: Q39043; GI:1303695; luminal
           binding protein (BiP) [Arabidopsis thaliana]
          Length = 613

 Score = 58.4 bits (135), Expect = 2e-09
 Identities = 24/55 (43%), Positives = 37/55 (67%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           L +K+   +K+ I     + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 535 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 589


>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
           similar to SWISS-PROT: Q39043; GI:1303695; luminal
           binding protein (BiP) [Arabidopsis thaliana]
          Length = 668

 Score = 58.4 bits (135), Expect = 2e-09
 Identities = 24/55 (43%), Positives = 37/55 (67%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           L +K+   +K+ I     + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 590 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644


>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
           SWISS-PROT:Q9LKR3 PMID:8888624
          Length = 669

 Score = 58.4 bits (135), Expect = 2e-09
 Identities = 24/55 (43%), Positives = 37/55 (67%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           L +K+   +K+ I     + ++WLD NQ ++KEEY+ K KE+E + NPIIT +YQ
Sbjct: 590 LADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQ 644


>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
           Similar to Arabidopsis luminal binding protein
           (gb|D89342); contains Pfam domain PF00012: dnaK protein
          Length = 678

 Score = 52.0 bits (119), Expect = 1e-07
 Identities = 21/55 (38%), Positives = 37/55 (67%)
 Frame = +1

Query: 1   LKEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           L +KISD DK+ +     + ++WL+ N  A+KE+Y+ K KE+E + +P+I  +Y+
Sbjct: 604 LAKKISDEDKEKMEGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPVIKSVYE 658


>At1g14650.1 68414.m01741 SWAP (Suppressor-of-White-APricot)/surp
           domain-containing protein / ubiquitin family protein
           similar to SP|Q15459 Splicing factor 3 subunit 1
           (Spliceosome associated protein 114) {Homo sapiens};
           contains Pfam profiles PF00240: Ubiquitin family,
           PF01805: Surp module
          Length = 785

 Score = 31.1 bits (67), Expect = 0.26
 Identities = 19/52 (36%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
 Frame = +1

Query: 1   LKEKI--SDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPII 150
           LKEK+  S +D  T+L++C   ++W D +Q   K++ E  +KELE +   +I
Sbjct: 252 LKEKLRKSAADLTTVLERCLHRLEW-DRSQEQQKKK-EEDEKELERVQMAMI 301


>At1g14640.1 68414.m01740 SWAP (Suppressor-of-White-APricot)/surp
           domain-containing protein similar to human splicing
           factor GB:CAA59494 GI:899298 from [Homo sapiens];
           contains Pfam profile PF01805: Surp module
          Length = 735

 Score = 28.7 bits (61), Expect = 1.4
 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
 Frame = +1

Query: 1   LKEKISDS--DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKE 123
           LKEK+  S  D  T+L++C + ++W    +    +E + K+KE
Sbjct: 243 LKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKE 285


>At5g01570.1 68418.m00072 hypothetical protein hypothetical protein
           T16O11.19 - Arabidopsis thaliana, EMBL:AC010871
          Length = 157

 Score = 27.9 bits (59), Expect = 2.5
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = +1

Query: 25  DKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELE 129
           D Q  LD C +     +S ++AD+EE E  QKEL+
Sbjct: 58  DLQKKLDVCKEKTDEANS-EIADEEEIERLQKELD 91


>At3g56850.1 68416.m06322 ABA-responsive element-binding protein 3
           (AREB3) identical to ABA-responsive element binding
           protein 3 (AREB3) [Arabidopsis thaliana] GI:9967421
          Length = 297

 Score = 27.9 bits (59), Expect = 2.5
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +2

Query: 95  RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
           R S + SR   +A+T     + +R+ EE+  + +    E   P VPPP
Sbjct: 236 RESAARSRARKQAYTHELEIKVSRLEEENERLRKQKEVEKILPSVPPP 283


>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
           to quiescin [Homo sapiens] GI:13257405; contains Pfam
           profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
           family
          Length = 495

 Score = 27.5 bits (58), Expect = 3.3
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = +1

Query: 67  WLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 165
           +L SNQ + + ++EH  K L+  Y P +  +Y+
Sbjct: 402 YLSSNQKSIEWDHEHVYKFLKNYYGPKLVSLYK 434


>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
            profile: PF00225 Kinesin motor domain; contains
            non-consensus splice site (GC) at intron 12
          Length = 2722

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 13/38 (34%), Positives = 23/38 (60%)
 Frame = +1

Query: 22   SDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGI 135
            +D++T + + ++ I  L+ +  A   EY HK KELE +
Sbjct: 2336 ADQKTEITQLSEHISELNLHAEAQASEYMHKFKELEAM 2373


>At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing
           protein 
          Length = 809

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 10/26 (38%), Positives = 13/26 (50%)
 Frame = +2

Query: 161 TRVPEESPEVCRASRAEHPEPEVPPP 238
           +R P  S +    SR   P P +PPP
Sbjct: 521 SRAPSSSAKRASGSRGRRPRPPLPPP 546


>At2g41070.3 68415.m05073 basic leucine zipper transcription factor
           (BZIP12) nearly identical to basic leucine zipper
           transcription factor [Arabidopsis thaliana] GI:21694632;
           contains a bZIP transcription factor basic domain
           signature (PDOC00036)
          Length = 262

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +2

Query: 95  RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
           R S + SR   +A+T     + +R+ EE+ ++ R    E   P  PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248


>At2g41070.2 68415.m05072 basic leucine zipper transcription factor
           (BZIP12) nearly identical to basic leucine zipper
           transcription factor [Arabidopsis thaliana] GI:21694632;
           contains a bZIP transcription factor basic domain
           signature (PDOC00036)
          Length = 262

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +2

Query: 95  RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
           R S + SR   +A+T     + +R+ EE+ ++ R    E   P  PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248


>At2g41070.1 68415.m05071 basic leucine zipper transcription factor
           (BZIP12) nearly identical to basic leucine zipper
           transcription factor [Arabidopsis thaliana] GI:21694632;
           contains a bZIP transcription factor basic domain
           signature (PDOC00036)
          Length = 262

 Score = 27.1 bits (57), Expect = 4.3
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +2

Query: 95  RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
           R S + SR   +A+T     + +R+ EE+ ++ R    E   P  PPP
Sbjct: 201 RESAARSRARKQAYTHELEIKVSRLEEENEKLRRLKEVEKILPSEPPP 248


>At4g29790.1 68417.m04242 expressed protein
          Length = 1211

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 5/36 (13%)
 Frame = +2

Query: 194 RASRAEHPEPE-----VPPPGLEALAHHRGGRLNQH 286
           R+ R E  E E     +P  G+ + AHH  GRL+ H
Sbjct: 789 RSDRLEFDELEDDVSVIPLKGVNSSAHHVNGRLSDH 824


>At3g62240.1 68416.m06992 zinc finger (C2H2 type) family protein
           contains Pfam PF00096: Zinc finger, C2H2 type
          Length = 812

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = +1

Query: 28  KQTILDKCNDTIKWLDSNQLADKEEYEHKQKE 123
           K+T+ D   DT++ L S+Q   +EE E   K+
Sbjct: 691 KETLADNFMDTVRRLQSSQNPQEEEEEAISKD 722


>At3g60000.1 68416.m06699 hypothetical protein  contains Pfam
           profile: PF04484 family of unknown function (DUF566)
          Length = 451

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 15/48 (31%), Positives = 22/48 (45%)
 Frame = +2

Query: 95  RRSMSTSRKNWKAFTIR*LRRCTRVPEESPEVCRASRAEHPEPEVPPP 238
           R S + +R     FT+   R+ ++     P    + R E P P VPPP
Sbjct: 88  RSSETAARSLDSPFTLSQSRKSSKPSHLKPLNENSHRLETPTPMVPPP 135


>At3g16565.1 68416.m02118 alanyl-tRNA synthetase-related low
           similarity to SP|Q56273 Alanyl-tRNA synthetase (EC
           6.1.1.7) (Alanine--tRNA ligase) (AlaRS) {Thiobacillus
           ferrooxidans}
          Length = 271

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +1

Query: 94  KEEYEHKQKELEGIYNPIITK 156
           +EE++ KQKELE   N +I+K
Sbjct: 172 QEEFQVKQKELEAEANELISK 192


>At2g37870.1 68415.m04649 protease inhibitor/seed storage/lipid
           transfer protein (LTP) family protein contains Pfam
           protease inhibitor/seed storage/LTP family domain
           PF00234
          Length = 115

 Score = 25.8 bits (54), Expect = 9.9
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -2

Query: 142 DCKCLPILSACAHTPPCRPAGWNP 71
           +C  +PI  A A   PC PA  NP
Sbjct: 26  ECGRMPINQAAASLSPCLPATKNP 49


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,298,465
Number of Sequences: 28952
Number of extensions: 130860
Number of successful extensions: 545
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 545
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 537681456
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -