Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30009
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S... 106 1e-23
At5g39740.1 68418.m04813 60S ribosomal protein L5 (RPL5B) riboso... 105 2e-23
At2g36610.1 68415.m04488 homeobox-leucine zipper family protein ... 29 3.6
At5g20550.1 68418.m02440 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 4.8
At4g36540.2 68417.m05189 basic helix-loop-helix (bHLH) family pr... 28 4.8
At4g36540.1 68417.m05188 basic helix-loop-helix (bHLH) family pr... 28 4.8
>At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S
ribosomal protein L5 GB:P49625 from [Oryza sativa]
Length = 301
Score = 106 bits (255), Expect = 1e-23
Identities = 48/76 (63%), Positives = 58/76 (76%)
Frame = +3
Query: 33 MGFVKVVKNKQYFKRYQVKFKRRREGKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDV 212
M FVK K+ YFKRYQVKF+RRR+GKTDY AR RL+ QDKNKYNTPKYR +VR +NKD+
Sbjct: 1 MVFVKSTKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDI 60
Query: 213 TCQVAYSRIEGDHICA 260
Q+ + I GD + A
Sbjct: 61 VAQIVSASIAGDIVKA 76
Score = 70.9 bits (166), Expect = 7e-13
Identities = 28/49 (57%), Positives = 41/49 (83%)
Frame = +1
Query: 511 GAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMR 657
GA+KGA+DGGL++PHS KRF G+ E+K+ +AE+HR +I+G HV+ YM+
Sbjct: 160 GALKGALDGGLDIPHSDKRFAGFHKENKQLDAEIHRNYIYGGHVSNYMK 208
Score = 67.7 bits (158), Expect = 6e-12
Identities = 36/80 (45%), Positives = 44/80 (55%)
Frame = +2
Query: 260 AAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXXEYNVEPVD 439
+AY+HELP+YG+ VGLTNYAAAY TG +++VEP D
Sbjct: 77 SAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGNVEATGEDFSVEPTD 136
Query: 440 NGPGAFRCYLDVGLARTTTG 499
+ FR LDVGL RTTTG
Sbjct: 137 S-RRPFRALLDVGLIRTTTG 155
>At5g39740.1 68418.m04813 60S ribosomal protein L5 (RPL5B) ribosomal
protein L5, rice
Length = 301
Score = 105 bits (253), Expect = 2e-23
Identities = 48/76 (63%), Positives = 58/76 (76%)
Frame = +3
Query: 33 MGFVKVVKNKQYFKRYQVKFKRRREGKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDV 212
M FVK K+ YFKRYQVKF+RRR+GKTDY AR RL+ QDKNKYNTPKYR +VR +NKD+
Sbjct: 1 MVFVKSSKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDI 60
Query: 213 TCQVAYSRIEGDHICA 260
Q+ + I GD + A
Sbjct: 61 VAQIVSASIAGDIVKA 76
Score = 70.9 bits (166), Expect = 7e-13
Identities = 28/49 (57%), Positives = 41/49 (83%)
Frame = +1
Query: 511 GAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAEVHRAHIFGLHVAEYMR 657
GA+KGA+DGGL++PHS KRF G+ E+K+ +AE+HR +I+G HV+ YM+
Sbjct: 160 GALKGALDGGLDIPHSDKRFAGFHKENKQLDAEIHRNYIYGGHVSNYMK 208
Score = 67.7 bits (158), Expect = 6e-12
Identities = 36/80 (45%), Positives = 44/80 (55%)
Frame = +2
Query: 260 AAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXXEYNVEPVD 439
+AY+HELP+YG+ VGLTNYAAAY TG +++VEP D
Sbjct: 77 SAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGNVEATGEDFSVEPTD 136
Query: 440 NGPGAFRCYLDVGLARTTTG 499
+ FR LDVGL RTTTG
Sbjct: 137 S-RRPFRALLDVGLIRTTTG 155
>At2g36610.1 68415.m04488 homeobox-leucine zipper family protein
similar to homeobox protein PpHB8 (GP:7415628)
[Physcomitrella patens]; contains PfamPF00046: Homeobox
domain
Length = 185
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 505 CLGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAE 609
CLG M GA L+VP ++ GY ES FN +
Sbjct: 35 CLGTMMGAQQDILHVPLAMVE-SGYGEESNSFNGQ 68
>At5g20550.1 68418.m02440 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to leucoanthocyanidin dioxygenase
[Malus domestica][SP|P51091], flavonol synthase [Petunia
x hybrida][GI:311658]; contains PF03171 2OG-Fe(II)
oxygenase superfamily domain
Length = 349
Score = 28.3 bits (60), Expect = 4.8
Identities = 10/41 (24%), Positives = 23/41 (56%)
Frame = -3
Query: 362 EAVFVLIADQLNMLQHNLSDQPSHHNVATHVNKQRTNMVTF 240
+ + + + DQ+ ++ + + P H V T+ K+R ++ TF
Sbjct: 260 DTILINVGDQMEIMSNGIYKSPVHR-VVTNTEKERISVATF 299
>At4g36540.2 68417.m05189 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 302
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +3
Query: 51 VKNKQYFKRYQVKFKRRREGKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTCQV 224
VKN + + ++ KR+ EGKT+ +K++ +D+ + P + +SN + + ++
Sbjct: 79 VKNNGHSRAITLQNKRKPEGKTEKREKKKIKAEDETE---PSMKGKSNMSNTETSSEI 133
>At4g36540.1 68417.m05188 basic helix-loop-helix (bHLH) family
protein contains Pfam domain, PF00010: Helix-loop-helix
DNA-binding domain
Length = 304
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +3
Query: 51 VKNKQYFKRYQVKFKRRREGKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTCQV 224
VKN + + ++ KR+ EGKT+ +K++ +D+ + P + +SN + + ++
Sbjct: 79 VKNNGHSRAITLQNKRKPEGKTEKREKKKIKAEDETE---PSMKGKSNMSNTETSSEI 133
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,417,769
Number of Sequences: 28952
Number of extensions: 290110
Number of successful extensions: 651
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 628
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 649
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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