Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00026
         (728 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g03430.1 68417.m00470 pre-mRNA splicing factor-related simila...    84   1e-16
At1g27440.1 68414.m03345 exostosin family protein contains Pfam ...    29   4.2  
At5g61840.1 68418.m07759 exostosin family protein contains Pfam ...    27   9.6  
At2g21300.1 68415.m02535 kinesin motor family protein contains P...    27   9.6  
At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3) s...    27   9.6  

>At4g03430.1 68417.m00470 pre-mRNA splicing factor-related similar
           to pre-mRNA splicing factor pre-mRNA splicing factor
           prp1 (SP:Q12381) [Fission yeast]
          Length = 1029

 Score = 83.8 bits (198), Expect = 1e-16
 Identities = 37/73 (50%), Positives = 53/73 (72%)
 Frame = +2

Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
           +GCE  P +E++WLEA RL   + A+ VIA   + +P+SV++W++AA LE + + K RV 
Sbjct: 412 RGCEECPKNEDVWLEACRLANPEDAKGVIAKGVKLIPNSVKLWLEAAKLEHDVENKSRVL 471

Query: 689 RKALEHIPNSVRL 727
           RK LEHIP+SVRL
Sbjct: 472 RKGLEHIPDSVRL 484



 Score = 40.7 bits (91), Expect = 0.001
 Identities = 24/68 (35%), Positives = 33/68 (48%)
 Frame = +2

Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
           KG E  P S  LW     L   + AR ++  A    P  + +WV  A LE  A++K +V 
Sbjct: 473 KGLEHIPDSVRLWKAVVELANEEDARILLHRAVECCPLHLELWVALARLETYAESK-KVL 531

Query: 689 RKALEHIP 712
            KA E +P
Sbjct: 532 NKAREKLP 539



 Score = 40.3 bits (90), Expect = 0.001
 Identities = 24/72 (33%), Positives = 36/72 (50%), Gaps = 4/72 (5%)
 Frame = +2

Query: 524 NPSSEELWLEAARLQPID----TARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFR 691
           NP +   W+ AAR++ +D     AR  I       P +  +W++A  L     AK  V  
Sbjct: 383 NPKNPNGWIAAARVEEVDGKIKAARFQIQRGCEECPKNEDVWLEACRLANPEDAK-GVIA 441

Query: 692 KALEHIPNSVRL 727
           K ++ IPNSV+L
Sbjct: 442 KGVKLIPNSVKL 453



 Score = 37.9 bits (84), Expect = 0.007
 Identities = 21/57 (36%), Positives = 29/57 (50%)
 Frame = +2

Query: 506 NKGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAK 676
           ++  E  P   ELW+  ARL+    ++ V+  A   LP    IW+ AA LE EA  K
Sbjct: 502 HRAVECCPLHLELWVALARLETYAESKKVLNKAREKLPKEPAIWITAAKLE-EANGK 557



 Score = 36.3 bits (80), Expect = 0.021
 Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
 Frame = +2

Query: 551 EAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKR---RVFRKALEHIPNS 718
           E  +   I+TARA+ AHA         IW+KAA LE+   ++     + RKA+ ++P +
Sbjct: 634 ECKKRGSIETARAIYAHALSVFLTKKSIWLKAAQLEKSHGSRESLDALLRKAVTYVPQA 692



 Score = 35.1 bits (77), Expect = 0.048
 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
 Frame = +2

Query: 527 PSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRR 682
           P +E LWL  A+ +     +  ARA++  A   +P+S  IW+ A  LE E K   R
Sbjct: 690 PQAEVLWLMGAKEKWLAGDVPAARAILQEAYAAIPNSEEIWLAAFKLEFENKEPER 745



 Score = 33.9 bits (74), Expect = 0.11
 Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
 Frame = +2

Query: 506 NKGCEVNPSSEELWLEAARLQP----IDTARAVIAHAARNLPHSVRIWVKAADLEQE 664
           N+G +  P+  +LWL   +L+     ++ AR       ++ PH + +W+  ADLE++
Sbjct: 784 NEGLKQFPTFFKLWLMLGQLEERFKHLEQARKAYDTGLKHCPHCIPLWLSLADLEEK 840



 Score = 31.9 bits (69), Expect = 0.45
 Identities = 22/80 (27%), Positives = 39/80 (48%), Gaps = 7/80 (8%)
 Frame = +2

Query: 500  SHNKGCEVNPSSEELWLEAARLQP----IDTARAVIAHAARNLPHSVRIWVKA--ADLEQ 661
            +++ G +  P    LWL  A L+     ++ ARA++  A +  P    +W+ A  A+L  
Sbjct: 816  AYDTGLKHCPHCIPLWLSLADLEEKVNGLNKARAILTTARKKNPGGAELWLAAIRAELRH 875

Query: 662  EAKAK-RRVFRKALEHIPNS 718
            + K +   +  KAL+  P S
Sbjct: 876  DNKREAEHLMSKALQDCPKS 895



 Score = 31.5 bits (68), Expect = 0.59
 Identities = 14/23 (60%), Positives = 18/23 (78%)
 Frame = +3

Query: 444 WIASARLEEVTGKVQTARNLIIR 512
           WIA+AR+EEV GK++ AR  I R
Sbjct: 390 WIAAARVEEVDGKIKAARFQIQR 412


>At1g27440.1 68414.m03345 exostosin family protein contains Pfam
           profile: PF03016 exostosin family
          Length = 412

 Score = 28.7 bits (61), Expect = 4.2
 Identities = 14/43 (32%), Positives = 20/43 (46%)
 Frame = +2

Query: 524 NPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAAD 652
           NPS +   L     QP D    ++   AR LPH   I++K  +
Sbjct: 354 NPSMKRAMLFPQPAQPGDAFHQILNGLARKLPHDKSIYLKTGE 396


>At5g61840.1 68418.m07759 exostosin family protein contains Pfam
           profile: PF03016 exostosin family ;supported by cDNA
           gi|23821293|dbj|AB080693.1|;
          Length = 415

 Score = 27.5 bits (58), Expect = 9.6
 Identities = 13/43 (30%), Positives = 21/43 (48%)
 Frame = +2

Query: 524 NPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAAD 652
           NPS ++  L     QP D    V+   AR LPH   ++++  +
Sbjct: 357 NPSMKQAMLFPQPAQPGDAFHQVLNGLARKLPHERSVYLRPGE 399


>At2g21300.1 68415.m02535 kinesin motor family protein contains Pfam
           profile: kinesin motor domain PF00225
          Length = 862

 Score = 27.5 bits (58), Expect = 9.6
 Identities = 15/33 (45%), Positives = 16/33 (48%)
 Frame = -3

Query: 384 KLKEHGASSCISGKRGRRCCNIWHWGSVDSISG 286
           K+ EH ASS       R   N W  GSV  ISG
Sbjct: 425 KMVEHDASSKAGTPHFRNRTNKWEDGSVSEISG 457


>At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3)
           strong similarity to dynamin-like protein 6 (ADL6)
           [Arabidopsis thaliana] GI:6651399; contains Pfam
           profiles PF01031: Dynamin central region, PF00350:
           Dynamin family, PF02212: Dynamin GTPase effector domain,
           PF00169: PH domain; identical to cDNA dynamin-like
           protein ADL3,  GI:4803835
          Length = 920

 Score = 27.5 bits (58), Expect = 9.6
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 574 RHGSCGHSSRCEEPASQCQDLGQSG 648
           R+GS GHS R  +PA   +D   SG
Sbjct: 872 RYGSGGHSRRYSDPAQNGEDSSGSG 896


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,060,724
Number of Sequences: 28952
Number of extensions: 264748
Number of successful extensions: 630
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 627
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)