Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_B05
(649 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme... 63 2e-10
At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 63 2e-10
At3g22460.1 68416.m02839 cysteine synthase, putative / O-acetyls... 62 3e-10
At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine (thi... 62 4e-10
At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine (thi... 62 4e-10
At3g59760.3 68416.m06669 cysteine synthase, mitochondrial, putat... 61 7e-10
At3g59760.2 68416.m06668 cysteine synthase, mitochondrial, putat... 61 7e-10
At3g59760.1 68416.m06667 cysteine synthase, mitochondrial, putat... 61 7e-10
At5g28020.2 68418.m03375 cysteine synthase, putative / O-acetyls... 59 3e-09
At5g28020.1 68418.m03374 cysteine synthase, putative / O-acetyls... 59 3e-09
At5g28030.2 68418.m03377 cysteine synthase, putative / O-acetyls... 56 2e-08
At5g28030.1 68418.m03376 cysteine synthase, putative / O-acetyls... 56 2e-08
At2g43750.1 68415.m05439 cysteine synthase, chloroplast / O-acet... 54 8e-08
At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ... 54 1e-07
At3g61440.1 68416.m06881 cysteine synthase, putative / O-acetyls... 51 6e-07
At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 51 8e-07
>At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 348
Score = 62.9 bits (146), Expect = 2e-10
Identities = 26/56 (46%), Positives = 41/56 (73%)
Frame = +3
Query: 426 NNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKS 593
N ++ IG+TPL+R+N + + G CE+ KCEF+NPGGS+KDR++ ++ EA +S
Sbjct: 42 NGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPGGSVKDRVAVKIIQEALES 95
>At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 421
Score = 62.9 bits (146), Expect = 2e-10
Identities = 26/56 (46%), Positives = 41/56 (73%)
Frame = +3
Query: 426 NNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKS 593
N ++ IG+TPL+R+N + + G CE+ KCEF+NPGGS+KDR++ ++ EA +S
Sbjct: 42 NGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPGGSVKDRVAVKIIQEALES 95
>At3g22460.1 68416.m02839 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative nearly identical over 185 amino
acids to SP|P47998 Cysteine synthase (EC 4.2.99.8)
(O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}
Length = 188
Score = 62.1 bits (144), Expect = 3e-10
Identities = 33/78 (42%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +3
Query: 417 RIYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSX 596
+I ++ IG+TPLV LN++ KD + AK E + P S+KDRI Y+M+ +A+
Sbjct: 7 KIAKDVTELIGNTPLVYLNKVAKD--CVGHVAAKLEMMEPCSSVKDRIGYSMIADAEAKG 64
Query: 597 IAKSG-TRFIEPTSXNTG 647
+ K G + IEPTS NTG
Sbjct: 65 LIKPGESVLIEPTSGNTG 82
>At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine
(thiol)-lyase / O-acetylserine sulfhydrylase (OAS1)
nearly identical to SP|P47998 Cysteine synthase (EC
4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA
O-acetylserine lyase (At.OAS.5-8) GI:6983573
Length = 322
Score = 61.7 bits (143), Expect = 4e-10
Identities = 33/79 (41%), Positives = 49/79 (62%), Gaps = 1/79 (1%)
Frame = +3
Query: 414 NRIYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKS 593
+RI ++ IG+TPLV LN + + G + AK E + P S+KDRI ++M+ +A+K
Sbjct: 3 SRIAKDVTELIGNTPLVYLNNVAE--GCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKK 60
Query: 594 XIAKSG-TRFIEPTSXNTG 647
+ K G + IEPTS NTG
Sbjct: 61 GLIKPGESVLIEPTSGNTG 79
>At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine
(thiol)-lyase / O-acetylserine sulfhydrylase (OAS1)
nearly identical to SP|P47998 Cysteine synthase (EC
4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA
O-acetylserine lyase (At.OAS.5-8) GI:6983573
Length = 322
Score = 61.7 bits (143), Expect = 4e-10
Identities = 33/79 (41%), Positives = 49/79 (62%), Gaps = 1/79 (1%)
Frame = +3
Query: 414 NRIYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKS 593
+RI ++ IG+TPLV LN + + G + AK E + P S+KDRI ++M+ +A+K
Sbjct: 3 SRIAKDVTELIGNTPLVYLNNVAE--GCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKK 60
Query: 594 XIAKSG-TRFIEPTSXNTG 647
+ K G + IEPTS NTG
Sbjct: 61 GLIKPGESVLIEPTSGNTG 79
>At3g59760.3 68416.m06669 cysteine synthase, mitochondrial, putative
/ O-acetylserine (thiol)-lyase, putative /
O-acetylserine sulfhydrylase, putative similar to
SP|Q43725 Cysteine synthase, mitochondrial precursor (EC
4.2.99.8) (O- acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana}
Length = 430
Score = 60.9 bits (141), Expect = 7e-10
Identities = 35/77 (45%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEA-QKSX 596
I +N+ IG TP+V LN I K G + AK E + P S+KDRI Y+MV +A QK
Sbjct: 113 IADNVSQLIGKTPMVYLNSIAK--GCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKGF 170
Query: 597 IAKSGTRFIEPTSXNTG 647
I+ + +EPTS NTG
Sbjct: 171 ISPGKSVLVEPTSGNTG 187
>At3g59760.2 68416.m06668 cysteine synthase, mitochondrial, putative
/ O-acetylserine (thiol)-lyase, putative /
O-acetylserine sulfhydrylase, putative similar to
SP|Q43725 Cysteine synthase, mitochondrial precursor (EC
4.2.99.8) (O- acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana}
Length = 432
Score = 60.9 bits (141), Expect = 7e-10
Identities = 35/77 (45%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEA-QKSX 596
I +N+ IG TP+V LN I K G + AK E + P S+KDRI Y+MV +A QK
Sbjct: 113 IADNVSQLIGKTPMVYLNSIAK--GCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKGF 170
Query: 597 IAKSGTRFIEPTSXNTG 647
I+ + +EPTS NTG
Sbjct: 171 ISPGKSVLVEPTSGNTG 187
>At3g59760.1 68416.m06667 cysteine synthase, mitochondrial, putative
/ O-acetylserine (thiol)-lyase, putative /
O-acetylserine sulfhydrylase, putative similar to
SP|Q43725 Cysteine synthase, mitochondrial precursor (EC
4.2.99.8) (O- acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana}
Length = 433
Score = 60.9 bits (141), Expect = 7e-10
Identities = 35/77 (45%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEA-QKSX 596
I +N+ IG TP+V LN I K G + AK E + P S+KDRI Y+MV +A QK
Sbjct: 113 IADNVSQLIGKTPMVYLNSIAK--GCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKGF 170
Query: 597 IAKSGTRFIEPTSXNTG 647
I+ + +EPTS NTG
Sbjct: 171 ISPGKSVLVEPTSGNTG 187
>At5g28020.2 68418.m03375 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 323
Score = 58.8 bits (136), Expect = 3e-09
Identities = 32/77 (41%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQ-KSX 596
I N+I IG+TP+V LN + G + AK E + P S+KDRI+Y+M+ +A+ K
Sbjct: 7 IKNDITELIGNTPMVYLNNVVD--GCVARIAAKLEMMEPCSSVKDRIAYSMIKDAEDKGL 64
Query: 597 IAKSGTRFIEPTSXNTG 647
I + IEPT+ NTG
Sbjct: 65 ITPGKSTLIEPTAGNTG 81
>At5g28020.1 68418.m03374 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 323
Score = 58.8 bits (136), Expect = 3e-09
Identities = 32/77 (41%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQ-KSX 596
I N+I IG+TP+V LN + G + AK E + P S+KDRI+Y+M+ +A+ K
Sbjct: 7 IKNDITELIGNTPMVYLNNVVD--GCVARIAAKLEMMEPCSSVKDRIAYSMIKDAEDKGL 64
Query: 597 IAKSGTRFIEPTSXNTG 647
I + IEPT+ NTG
Sbjct: 65 ITPGKSTLIEPTAGNTG 81
>At5g28030.2 68418.m03377 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase [Brassica juncea] GI:2245144; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 323
Score = 56.4 bits (130), Expect = 2e-08
Identities = 32/77 (41%), Positives = 45/77 (58%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQ-KSX 596
I N++ IG+TP+V LN+I G + AK E + P SIKDRI+Y+M+ +A+ K
Sbjct: 7 IKNDVTELIGNTPMVYLNKIVD--GCVARIAAKLEMMEPCSSIKDRIAYSMIKDAEDKGL 64
Query: 597 IAKSGTRFIEPTSXNTG 647
I + IE T NTG
Sbjct: 65 ITPGKSTLIEATGGNTG 81
>At5g28030.1 68418.m03376 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase [Brassica juncea] GI:2245144; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 323
Score = 56.4 bits (130), Expect = 2e-08
Identities = 32/77 (41%), Positives = 45/77 (58%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQ-KSX 596
I N++ IG+TP+V LN+I G + AK E + P SIKDRI+Y+M+ +A+ K
Sbjct: 7 IKNDVTELIGNTPMVYLNKIVD--GCVARIAAKLEMMEPCSSIKDRIAYSMIKDAEDKGL 64
Query: 597 IAKSGTRFIEPTSXNTG 647
I + IE T NTG
Sbjct: 65 ITPGKSTLIEATGGNTG 81
>At2g43750.1 68415.m05439 cysteine synthase, chloroplast /
O-acetylserine (thiol)-lyase / O-acetylserine
sulfhydrylase / cpACS1 (OASB) identical to SP|P47999
Cysteine synthase, chloroplast precursor (EC 4.2.99.8)
(O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) (cpACS1) {Arabidopsis thaliana};
identical to cDNA O-acetylserine lyase (At.OAS.7-4)
GI:6983575
Length = 392
Score = 54.0 bits (124), Expect = 8e-08
Identities = 31/77 (40%), Positives = 43/77 (55%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEA-QKSX 596
I +N IG TP+V LN + K G + AK E + P S+KDRI Y+M+ +A +K
Sbjct: 75 IADNAAQLIGKTPMVYLNNVVK--GCVASVAAKLEIMEPCCSVKDRIGYSMITDAEEKGL 132
Query: 597 IAKSGTRFIEPTSXNTG 647
I + +E TS NTG
Sbjct: 133 ITPGKSVLVESTSGNTG 149
>At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to SP|O22682 Probable
cysteine synthase, chloroplast precursor {Arabidopsis
thaliana}, similar to SP|P31300 Cysteine synthase,
chloroplast precursor {Capsicum annuum}
Length = 404
Score = 53.6 bits (123), Expect = 1e-07
Identities = 31/77 (40%), Positives = 44/77 (57%), Gaps = 1/77 (1%)
Frame = +3
Query: 420 IYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKS-X 596
I ++ IG TP+V LNR+ G ++ AK E + P S+KDRI +M+ EA+ S
Sbjct: 99 IAEDVTQLIGSTPMVYLNRVTD--GCLADIAAKLESMEPCRSVKDRIGLSMINEAENSGA 156
Query: 597 IAKSGTRFIEPTSXNTG 647
I T +EPT+ NTG
Sbjct: 157 ITPRKTVLVEPTTGNTG 173
>At3g61440.1 68416.m06881 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to cysteine synthase
(EC 4.2.99.8) [Arabidopsis thaliana] GI:5824334;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 368
Score = 51.2 bits (117), Expect = 6e-07
Identities = 31/69 (44%), Positives = 40/69 (57%), Gaps = 1/69 (1%)
Frame = +3
Query: 444 IGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSXIAKSG-TRF 620
IG TPLV LN++ + G E + AK E P SIKDR + M+ +A+K + G T
Sbjct: 57 IGKTPLVFLNKVTE--GCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTL 114
Query: 621 IEPTSXNTG 647
IEPTS N G
Sbjct: 115 IEPTSGNMG 123
>At3g04940.1 68416.m00536 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative similar to O-acetylserine(thiol)
lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 324
Score = 50.8 bits (116), Expect = 8e-07
Identities = 31/80 (38%), Positives = 44/80 (55%), Gaps = 1/80 (1%)
Frame = +3
Query: 411 RNRIYNNILXTIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQ- 587
R I ++ IG+TP+V LN I G + AK E + P S+K+RI+Y M+ +A+
Sbjct: 5 RCSIKDDATQLIGNTPMVYLNNIVD--GCVARIAAKLEMMEPCSSVKERIAYGMIKDAED 62
Query: 588 KSXIAKSGTRFIEPTSXNTG 647
K I + IE TS NTG
Sbjct: 63 KGLITPGKSTLIEATSGNTG 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,271,110
Number of Sequences: 28952
Number of extensions: 221593
Number of successful extensions: 428
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 399
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 409
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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