Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_B03
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g33560.1 68415.m04113 spindle checkpoint protein-related simi...    70   1e-12
At5g05510.1 68418.m00598 protein kinase-related low similarity t...    35   0.054
At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)...    27   8.2  
At2g34150.1 68415.m04180 expressed protein                             27   8.2  

>At2g33560.1 68415.m04113 spindle checkpoint protein-related similar
           to spindle checkpoint protein BubR1 (GI:22128593)
           [Xenopus laevis]; similar to Mitotic checkpoint
           serine/threonine-protein kinase BUB1 beta (EC 2.7.1.-)
           (MAD3/BUB1-related protein kinase) (Mitotic checkpoint
           kinase MAD3L) (Swiss-Prot:Q9Z1S0) [Mus musculus]
          Length = 395

 Score = 70.1 bits (164), Expect = 1e-12
 Identities = 32/95 (33%), Positives = 55/95 (57%)
 Frame = +2

Query: 365 DFXKENIQPLRGGRNLVQLSTALQAQSDADAQKQLQIQKEEHETAIXNYQXPDPLEPWFN 544
           +  KEN++PL+ GRN+  L+ AL++ SD   +K L  ++     AI  Y+  DPL PW  
Sbjct: 28  ELFKENVRPLKRGRNVGILNHALKSHSDHQLRKNLIEKRRNLIEAIDEYEGDDPLSPWIE 87

Query: 545 YIQWVEQSFPKHGHEXHIDKLIKXCLQLFENDNKY 649
            I+WV+++FP  G    +  + + C++ F +  +Y
Sbjct: 88  CIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERY 122


>At5g05510.1 68418.m00598 protein kinase-related low similarity to
           SP|O60566 Mitotic checkpoint serine/threonine-protein
           kinase BUB1 beta (EC 2.7.1.-) {Homo sapiens}
          Length = 471

 Score = 34.7 bits (76), Expect = 0.054
 Identities = 13/50 (26%), Positives = 26/50 (52%)
 Frame = +2

Query: 500 IXNYQXPDPLEPWFNYIQWVEQSFPKHGHEXHIDKLIKXCLQLFENDNKY 649
           I +Y   D L PW   ++ +++S P       + + ++ C + FE+D +Y
Sbjct: 19  IKSYSGKDHLLPWIRGVKKMKESLPSQILNEKLPRFLQKCAESFESDKRY 68


>At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)
           family protein similar to SP|P11931 Valyl-tRNA
           synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS)
           {Bacillus stearothermophilus}; contains Pfam profile
           PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 970

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = +2

Query: 455 AQKQLQIQKEEHETAIXNYQXPDPLEPWFNYIQW 556
           A++ L+   E++   +  YQ PD L+ WF+   W
Sbjct: 494 AEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLW 527


>At2g34150.1 68415.m04180 expressed protein
          Length = 700

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
 Frame = +2

Query: 521 DPLEPWFNYIQWVEQSFPKHGHEXHIDKLIKXCL-QLFENDN 643
           +PL P    +QW+    P  G E +  + +K  L Q FE +N
Sbjct: 549 EPLPPPLPPLQWMVSKIPSAGFEDNNKQSLKDALTQAFEKNN 590


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,802,076
Number of Sequences: 28952
Number of extensions: 207186
Number of successful extensions: 402
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 402
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)