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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_A22
         (706 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g20080.1 68414.m02513 C2 domain-containing protein contains I...    55   5e-08
At2g20990.1 68415.m02485 C2 domain-containing protein (sytA) sim...    51   9e-07
At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein...    50   2e-06
At5g04220.2 68418.m00411 C2 domain-containing protein (sytC) GC ...    46   2e-05
At5g11100.1 68418.m01296 C2 domain-containing protein similar to...    39   0.003
At1g05500.1 68414.m00561 C2 domain-containing protein similar to...    33   0.18 
At5g02390.1 68418.m00162 expressed protein ; expression supporte...    29   3.0  
At3g61030.1 68416.m06828 C2 domain-containing protein similar to...    29   3.0  
At3g60950.1 68416.m06819 C2 domain-containing protein similar to...    29   3.0  
At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-r...    28   5.2  
At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-r...    28   5.2  
At5g28860.1 68418.m03551 hypothetical protein                          28   5.2  
At1g72840.1 68414.m08425 disease resistance protein (TIR-NBS-LRR...    28   5.2  
At5g36930.1 68418.m04427 disease resistance protein (TIR-NBS-LRR...    28   6.9  
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical...    28   6.9  
At3g51850.1 68416.m05686 calcium-dependent protein kinase, putat...    27   9.2  
At1g69860.1 68414.m08040 proton-dependent oligopeptide transport...    27   9.2  

>At1g20080.1 68414.m02513 C2 domain-containing protein contains
           INTERPRO:IPR000008 C2 domain
          Length = 535

 Score = 54.8 bits (126), Expect = 5e-08
 Identities = 19/63 (30%), Positives = 37/63 (58%)
 Frame = +3

Query: 504 EKDVILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLKDLIEPAVAESLAN 683
           + + I     ++P WV  PD +R +WLN+++  +WP ++     + K + +P +AE + N
Sbjct: 46  DSETIATMFPEIPMWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPN 105

Query: 684 YKL 692
           YK+
Sbjct: 106 YKI 108


>At2g20990.1 68415.m02485 C2 domain-containing protein (sytA)
           similar to Ca2+-dependent lipid-binding protein (CLB1)
           GI:2789434 from [Lycopersicon esculentum]
          Length = 541

 Score = 50.8 bits (116), Expect = 9e-07
 Identities = 27/106 (25%), Positives = 51/106 (48%)
 Frame = +3

Query: 375 LVGYMQWSVAWLIGPVILSVMRDQWRKENEYKRNIAKVAALSSEKDVILARLDDLPSWVF 554
           ++G+  + V   +G VI  V+       +     I  +A    +   +L  L ++P WV 
Sbjct: 7   ILGFCGFGVGISLGLVIGYVLFVYLLPNDVKDPEIRSIA--DQDPKAMLRMLPEIPLWVK 64

Query: 555 FPDVERAEWLNRILLQVWPNVNSYAKTLLKDLIEPAVAESLANYKL 692
            PD +R +W+NR L  +WP ++       K++ +P + E +  YK+
Sbjct: 65  NPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKI 110


>At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein,
           putative strong similarity to CLB1 [Lycopersicon
           esculentum] GI:2789434; contains Pfam profile PF00168:
           C2 domain
          Length = 510

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 16/47 (34%), Positives = 31/47 (65%)
 Frame = +3

Query: 531 DDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLKDLIEPAVAE 671
           D+ P W+ FP  E+ +WLN++L ++WP +   A  +++D +EP + +
Sbjct: 56  DNFPQWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPLLED 102


>At5g04220.2 68418.m00411 C2 domain-containing protein (sytC) GC
           donor splice site at exon 3; similar to Ca2+-dependent
           lipid-binding protein (CLB1) GI:2789434 from
           [Lycopersicon esculentum]
          Length = 540

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 16/58 (27%), Positives = 33/58 (56%)
 Frame = +3

Query: 513 VILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLKDLIEPAVAESLANY 686
           V+L  L D+P W+  PD ER +W N+ +  +WP ++     +++  ++P  A+ +  +
Sbjct: 51  VLLDLLPDIPLWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTF 108


>At5g11100.1 68418.m01296 C2 domain-containing protein similar to
           Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
           from [Lycopersicon esculentum]
          Length = 574

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 23/74 (31%), Positives = 35/74 (47%), Gaps = 7/74 (9%)
 Frame = +3

Query: 471 RNIAKVAALSSEKDVILARLDDLPSWVFFPDVERAE-------WLNRILLQVWPNVNSYA 629
           + IA  A ++ +    L   D  PSWV F   ++         WLN  L ++WP VN  A
Sbjct: 37  KTIAAFARMTVQDSRKLLPGDFYPSWVVFSQRQKLSYSKCLLNWLNLELEKIWPYVNEAA 96

Query: 630 KTLLKDLIEPAVAE 671
             L+K  +EP + +
Sbjct: 97  SELIKSSVEPVLEQ 110


>At1g05500.1 68414.m00561 C2 domain-containing protein similar to
           Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
           from [Lycopersicon esculentum]
          Length = 528

 Score = 33.1 bits (72), Expect = 0.18
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = +3

Query: 567 ERAEWLNRILLQVWPNVNSYAKTLLKDLIEPAVAE 671
           E   WLN  L ++WP V+  A  L+K  +EP + +
Sbjct: 34  ELLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQ 68


>At5g02390.1 68418.m00162 expressed protein ; expression supported
           by MPSS
          Length = 835

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 15/65 (23%), Positives = 31/65 (47%)
 Frame = -1

Query: 619 FTFGQTCRSILFNHSARSTSGKNTHEGKSSSLASITSFSEDSAATFAMLRLYSFSLRH*S 440
           + F +  R  +F H   +++ KN ++ + + L  IT  S    +    L+LY  S +  +
Sbjct: 440 YGFSKDLRDDIFEHLKGNSAAKNNNKAEGTKLKQITRASSLCGSLDRYLQLYECSFQREA 499

Query: 439 RITDN 425
           + + N
Sbjct: 500 KNSSN 504


>At3g61030.1 68416.m06828 C2 domain-containing protein similar to
           CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam
           profile PF00168: C2 domain
          Length = 592

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 9/32 (28%), Positives = 21/32 (65%)
 Frame = +3

Query: 576 EWLNRILLQVWPNVNSYAKTLLKDLIEPAVAE 671
           +WLN++L ++WP +   A  +++  +EP + +
Sbjct: 40  KWLNKLLSKMWPYIAEAATMVVRYSVEPLLED 71


>At3g60950.1 68416.m06819 C2 domain-containing protein similar to
           CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam
           profile PF00168: C2 domain
          Length = 592

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 9/32 (28%), Positives = 21/32 (65%)
 Frame = +3

Query: 576 EWLNRILLQVWPNVNSYAKTLLKDLIEPAVAE 671
           +WLN++L ++WP +   A  +++  +EP + +
Sbjct: 40  KWLNKLLSKMWPYIAEAATMVVRYSVEPLLED 71


>At5g35210.2 68418.m04175 peptidase M50 family protein /
           sterol-regulatory element binding protein (SREBP) site 2
           protease family protein contains PFam PF02163:
           sterol-regulatory element binding protein (SREBP) site 2
           protease
          Length = 1409

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
 Frame = +3

Query: 240 CSMASDNNMAGASKTALPPGSDDGNSVFSMMYTFLKKVSIVGAV--YLVGYMQWSVAWLI 413
           C + S +        A+   +     +FS ++       ++ ++  Y++ Y++ S+  LI
Sbjct: 730 CRLTSASRRGCMLNAAVAGATKGAMKIFSGLFPLKNGEGVLSSIAAYIL-YLEESLRGLI 788

Query: 414 -GPVILSVMRDQWRKENEYKRNIAKVA 491
            GP +    R QWRK+ E   NI  +A
Sbjct: 789 AGPFLSESPRKQWRKQLE--ENICSIA 813


>At5g35210.1 68418.m04174 peptidase M50 family protein /
           sterol-regulatory element binding protein (SREBP) site 2
           protease family protein contains PFam PF02163:
           sterol-regulatory element binding protein (SREBP) site 2
           protease
          Length = 1576

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
 Frame = +3

Query: 240 CSMASDNNMAGASKTALPPGSDDGNSVFSMMYTFLKKVSIVGAV--YLVGYMQWSVAWLI 413
           C + S +        A+   +     +FS ++       ++ ++  Y++ Y++ S+  LI
Sbjct: 730 CRLTSASRRGCMLNAAVAGATKGAMKIFSGLFPLKNGEGVLSSIAAYIL-YLEESLRGLI 788

Query: 414 -GPVILSVMRDQWRKENEYKRNIAKVA 491
            GP +    R QWRK+ E   NI  +A
Sbjct: 789 AGPFLSESPRKQWRKQLE--ENICSIA 813


>At5g28860.1 68418.m03551 hypothetical protein
          Length = 183

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
 Frame = +3

Query: 426 LSVMRDQWRKENEYKRNI-AKVAALSSEKDVILARLDDLPSWVFFPDVERAEWLNRILLQ 602
           L   R++ RK +     + A+VA + +++  + ARLD+    V   ++   +W    L  
Sbjct: 104 LQYFRNKKRKVDSRPEELEAEVAKIRAKQKKVTARLDEFKQMVKALEILSEDWEKVDLQL 163

Query: 603 VWP 611
           VWP
Sbjct: 164 VWP 166


>At1g72840.1 68414.m08425 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1183

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/25 (48%), Positives = 16/25 (64%)
 Frame = +2

Query: 326 YDVHISQKGVDCRSCVLSRLYAMER 400
           YDV +S +GVD R  ++S LY   R
Sbjct: 15  YDVFLSFRGVDTRQTIVSHLYVALR 39


>At5g36930.1 68418.m04427 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1188

 Score = 27.9 bits (59), Expect = 6.9
 Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
 Frame = +2

Query: 326 YDVHISQKGVDCRSCVLSRLY-AMERC 403
           YDV +S +G D R   LS LY ++ RC
Sbjct: 14  YDVFVSFRGADVRKNFLSHLYDSLRRC 40


>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
            EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
            member of the natural resistance-associated macrophage
            protein (NRAMP) metal transporter family, PMID:11500563;
            metal transport capacity has not been shown,
            PMID:11500563, PMID:1038174
          Length = 1294

 Score = 27.9 bits (59), Expect = 6.9
 Identities = 13/44 (29%), Positives = 23/44 (52%)
 Frame = -2

Query: 558  GRIPMKASRQVSLVSHLFQKTVQRPLRCCVCIRSPCAIDRASPT 427
            G+     +R   ++   F K ++ P+  C C++ P +  RASPT
Sbjct: 1159 GKYTYVLNRLQGVIDPAFSK-LRTPMTPCFCLQIPASHQRASPT 1201


>At3g51850.1 68416.m05686 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calcium-dependent protein
           kinase [Arabidopsis thaliana] gi|836942|gb|AAA67655;
           contains protein kinase domain, Pfam:PF00069; contains
           EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
           INTERPRO:IPR002048
          Length = 528

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 8/18 (44%), Positives = 14/18 (77%)
 Frame = +3

Query: 606 WPNVNSYAKTLLKDLIEP 659
           WPN++  AK L++ ++EP
Sbjct: 278 WPNISETAKNLVRQMLEP 295


>At1g69860.1 68414.m08040 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 555

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 17/47 (36%), Positives = 27/47 (57%)
 Frame = +3

Query: 297 GSDDGNSVFSMMYTFLKKVSIVGAVYLVGYMQWSVAWLIGPVILSVM 437
           G +   S FS  YT    V +V ++ LV Y+Q +++W IG  I +V+
Sbjct: 174 GREGSRSFFSWYYTTHTIVQLV-SMTLVLYVQNNISWGIGFAIPTVL 219


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,783,107
Number of Sequences: 28952
Number of extensions: 300616
Number of successful extensions: 823
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 801
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 823
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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